METHOD OF PRODUCING A RECOMBINANT PEPTIDE
Methods of producing a recombinant peptide, such as vessel dilator peptide (VSDL), are disclosed, including a particular method involving expressing a fusion polypeptide comprising concatemeric repeats of the peptide wherein the peptide is flanked by peptide cleavage sites, and cleaving the fusion p...
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| creator | RAISHEED, SAIF BASTIRAS, STAN ZAREIE, REZA HUNT, BEN GUIDOLIN, ANGELO |
| description | Methods of producing a recombinant peptide, such as vessel dilator peptide (VSDL), are disclosed, including a particular method involving expressing a fusion polypeptide comprising concatemeric repeats of the peptide wherein the peptide is flanked by peptide cleavage sites, and cleaving the fusion polypeptide at the peptide cleavage sites with a cleaving agent(s) so as to release the peptide from the fusion polypeptide. Expression constructs and host cells for producing the fusion polypeptide and purification methods for the recombinant peptide are also disclosed.
L'invention concerne des procédés de production d'un peptide recombinant tel qu'un peptide dilatateur de vaisseau (VSDL), notamment un procédé impliquant l'expression d'un polypeptide de fusion comprenant des répétitions concatémères du peptide, le peptide étant flanqué de sites de clivage de peptide, et le clivage du polypeptide de fusion au niveau des sites de clivage de peptide avec un ou plusieurs agents de clivage de manière à libérer le peptide du polypeptide de fusion. Des constructions d'expression et des cellules pour la production du polypeptide de fusion ainsi que des procédés de purification pour le peptide recombinant sont également divulgués. |
| format | Patent |
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L'invention concerne des procédés de production d'un peptide recombinant tel qu'un peptide dilatateur de vaisseau (VSDL), notamment un procédé impliquant l'expression d'un polypeptide de fusion comprenant des répétitions concatémères du peptide, le peptide étant flanqué de sites de clivage de peptide, et le clivage du polypeptide de fusion au niveau des sites de clivage de peptide avec un ou plusieurs agents de clivage de manière à libérer le peptide du polypeptide de fusion. Des constructions d'expression et des cellules pour la production du polypeptide de fusion ainsi que des procédés de purification pour le peptide recombinant sont également divulgués.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2013</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20130926&DB=EPODOC&CC=WO&NR=2013138850A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,782,887,25571,76555</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20130926&DB=EPODOC&CC=WO&NR=2013138850A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>RAISHEED, SAIF</creatorcontrib><creatorcontrib>BASTIRAS, STAN</creatorcontrib><creatorcontrib>ZAREIE, REZA</creatorcontrib><creatorcontrib>HUNT, BEN</creatorcontrib><creatorcontrib>GUIDOLIN, ANGELO</creatorcontrib><title>METHOD OF PRODUCING A RECOMBINANT PEPTIDE</title><description>Methods of producing a recombinant peptide, such as vessel dilator peptide (VSDL), are disclosed, including a particular method involving expressing a fusion polypeptide comprising concatemeric repeats of the peptide wherein the peptide is flanked by peptide cleavage sites, and cleaving the fusion polypeptide at the peptide cleavage sites with a cleaving agent(s) so as to release the peptide from the fusion polypeptide. Expression constructs and host cells for producing the fusion polypeptide and purification methods for the recombinant peptide are also disclosed.
L'invention concerne des procédés de production d'un peptide recombinant tel qu'un peptide dilatateur de vaisseau (VSDL), notamment un procédé impliquant l'expression d'un polypeptide de fusion comprenant des répétitions concatémères du peptide, le peptide étant flanqué de sites de clivage de peptide, et le clivage du polypeptide de fusion au niveau des sites de clivage de peptide avec un ou plusieurs agents de clivage de manière à libérer le peptide du polypeptide de fusion. Des constructions d'expression et des cellules pour la production du polypeptide de fusion ainsi que des procédés de purification pour le peptide recombinant sont également divulgués.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2013</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZND0dQ3x8HdR8HdTCAjydwl19vRzV3BUCHJ19vd18vRz9AtRCHANCPF0ceVhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHh_kYGhsaGxhYWpgaOhsbEqQIAqaglHQ</recordid><startdate>20130926</startdate><enddate>20130926</enddate><creator>RAISHEED, SAIF</creator><creator>BASTIRAS, STAN</creator><creator>ZAREIE, REZA</creator><creator>HUNT, BEN</creator><creator>GUIDOLIN, ANGELO</creator><scope>EVB</scope></search><sort><creationdate>20130926</creationdate><title>METHOD OF PRODUCING A RECOMBINANT PEPTIDE</title><author>RAISHEED, SAIF ; BASTIRAS, STAN ; ZAREIE, REZA ; HUNT, BEN ; GUIDOLIN, ANGELO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2013138850A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2013</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>RAISHEED, SAIF</creatorcontrib><creatorcontrib>BASTIRAS, STAN</creatorcontrib><creatorcontrib>ZAREIE, REZA</creatorcontrib><creatorcontrib>HUNT, BEN</creatorcontrib><creatorcontrib>GUIDOLIN, ANGELO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>RAISHEED, SAIF</au><au>BASTIRAS, STAN</au><au>ZAREIE, REZA</au><au>HUNT, BEN</au><au>GUIDOLIN, ANGELO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD OF PRODUCING A RECOMBINANT PEPTIDE</title><date>2013-09-26</date><risdate>2013</risdate><abstract>Methods of producing a recombinant peptide, such as vessel dilator peptide (VSDL), are disclosed, including a particular method involving expressing a fusion polypeptide comprising concatemeric repeats of the peptide wherein the peptide is flanked by peptide cleavage sites, and cleaving the fusion polypeptide at the peptide cleavage sites with a cleaving agent(s) so as to release the peptide from the fusion polypeptide. Expression constructs and host cells for producing the fusion polypeptide and purification methods for the recombinant peptide are also disclosed.
L'invention concerne des procédés de production d'un peptide recombinant tel qu'un peptide dilatateur de vaisseau (VSDL), notamment un procédé impliquant l'expression d'un polypeptide de fusion comprenant des répétitions concatémères du peptide, le peptide étant flanqué de sites de clivage de peptide, et le clivage du polypeptide de fusion au niveau des sites de clivage de peptide avec un ou plusieurs agents de clivage de manière à libérer le peptide du polypeptide de fusion. Des constructions d'expression et des cellules pour la production du polypeptide de fusion ainsi que des procédés de purification pour le peptide recombinant sont également divulgués.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD OF PRODUCING A RECOMBINANT PEPTIDE |
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