PROCESS FOR PREPARING FGF-21 WITH LOW DEGREE OF O-GLYCOSYLATION

Expression of FGF21 in a S. cerevisiae pmt2 knock out strain reduces the O-glycosylation significantly and does not decrease the expression level. Expression in mkc7 knock out strain can decrease degradation of FGF21 and analogues thereof. Point mutations at position R17 and R36 can decrease degrada...

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Hauptverfasser:	SLAABY, RITA, LAUTRUP-LARSEN, INGER
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Zusammenfassung:	Expression of FGF21 in a S. cerevisiae pmt2 knock out strain reduces the O-glycosylation significantly and does not decrease the expression level. Expression in mkc7 knock out strain can decrease degradation of FGF21 and analogues thereof. Point mutations at position R17 and R36 can decrease degradation of FGF21 and analogues thereof when they are expressed in yeast. L'expression de FGF21 dans une souche knock out pmt2 de S. cerevisiae réduit de manière significative la O-glycosylation et ne fait pas diminuer le niveau d'expression. L'expression dans la souche knock out mkc7 peut faire diminuer la dégradation de FGF21 et de ses analogues. Des mutations ponctuelles en position R17 et R36 peuvent faire diminuer la dégradation de FGF21 et de ses analogues lorsqu'elles sont exprimées dans la levure.