AUTOMATED INOCULATION MECHANISM WITH INTERMITTENT STIRRING SYSTEM
Blood culture method for cultivating a patient's blood, and equipment characterised in that, in a single apparatus, an inherent CO2 indicator prepared in a solid agar surface, which allows early diagnosis of seropositivity, is used together with two solid media which allow pathogenic bacteria o...
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| description | Blood culture method for cultivating a patient's blood, and equipment characterised in that, in a single apparatus, an inherent CO2 indicator prepared in a solid agar surface, which allows early diagnosis of seropositivity, is used together with two solid media which allow pathogenic bacteria or fungi to develop without the need to vent the culture bottles. Thus, two bacterial growth indicators (CO2 generation and growth in solid media) are used in the same apparatus, which is formed by a main body in the lower part thereof containing a bottle, from the central part of which an oblique sampling duct projects, said bottle containing a liquid medium and receiving in the upper part thereof a fixed bulb which contains two solid medium plates and one CO2 indicator plate of green colour or of any other colour. In order to facilitate the diagnostic, the manipulator injects blood by means of a syringe into the sampling duct, which leads the blood into the bottle, where the blood is mixed with the liquid medium, then the manipulator turns the apparatus upside down, causing the mixture to fall inside the bulb and come into contact with the plates, whereon two solid media promote the growth of colonies of pathogenic bacteria or fungi, while the CO2 indicator plate changes colour in the presence of the gas. The intermittent stirring process produced in the stove promotes microbial growth and the automated and programmed inversion of the bottles makes inoculation into solid media easier, dispensing with the need for a manipulator in the laboratory for carrying out this step.
L'invention concerne un procédé d'hémoculture pour la mise en culture du sang d'un patient ainsi qu'un équipement associé. L'invention se caractérise en ce qu'on utilise, dans le même équipement, un indicateur de CO2, préparé sur une surface solide avec de l'agar, ce qui permet un diagnostic précoce de positivité, et deux milieux solides permettant le développement de bactéries ou de champignons sans nécessité de soumettre les flacons à une aspiration. Ainsi, deux indicateurs de croissance bactérienne (production de CO2 et multiplication en milieux solides) sont utilisés dans le même équipement, lequel est constitué par un corps principal pourvu inférieurement d'un flacon dont la partie médiane présente un conduit collecteur faisant saillie obliquement. La partie intérieure dudit flacon sert à contenir un milieu liquide, la partie supérieure recevant de manière fixe une ampoule qui renferme deux pl |
| format | Patent |
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L'invention concerne un procédé d'hémoculture pour la mise en culture du sang d'un patient ainsi qu'un équipement associé. L'invention se caractérise en ce qu'on utilise, dans le même équipement, un indicateur de CO2, préparé sur une surface solide avec de l'agar, ce qui permet un diagnostic précoce de positivité, et deux milieux solides permettant le développement de bactéries ou de champignons sans nécessité de soumettre les flacons à une aspiration. Ainsi, deux indicateurs de croissance bactérienne (production de CO2 et multiplication en milieux solides) sont utilisés dans le même équipement, lequel est constitué par un corps principal pourvu inférieurement d'un flacon dont la partie médiane présente un conduit collecteur faisant saillie obliquement. La partie intérieure dudit flacon sert à contenir un milieu liquide, la partie supérieure recevant de manière fixe une ampoule qui renferme deux plaques de milieux solides et une plaque indicatrice de CO2, de couleur verte ou d'une autre couleur quelconque. Pour faciliter le diagnostic, le manipulateur injecte le sang par l'intermédiaire d'une seringue dans le conduit collecteur, le sang étant alors acheminé dans le flacon, où il est mélangé avec le milieu liquide. Le manipulateur retourne ensuite l'équipement, ce qui amène le mélange à entrer dans l'ampoule, où il vient au contact des plaques, les milieux solides favorisant l'apparition de colonies de bactéries ou de champignons pathogènes. Dans le même temps, la plaque indicatrice de CO2 change de couleur en présence du gaz. Un procédé d'agitation intermittente réalisé dans une étuve favorise la multiplication microbienne, et l'inversion automatisée et programmée des flacons favorise l'ensemencement dans des milieux solides, ce qui supprime la nécessité de faire intervenir un manipulateur pour cette étape dans le laboratoire.
Processo de hemocultura para cultivo do sangue de um paciente e equipamento utilizado caracterizado por utilizar no mesmo equipamento um indicador próprio de CO2, preparado numa superfície sólida com agar, o que permite um diagnóstico precoce da positividade, e de dois meios sólidos que permitem sem necessidade de aspirar os frascos o desenvolvimento de bactérias ou fungos patogênicos, assim através de dois indicadores de crescimento bacteriano, (produção de CO2 e multiplicação em meios sólidos) usados no mesmo equipamento esse constituído por um corpo principal que detém inferiormente um frasco que medianamente projeta obliquamente um duto coletor sendo que internamente o dito frasco acondiciona um meio liquido e superiormente recebe de forma fixa um bulbo que internamente detém duas placas de meio sólidos e uma placa indicadora de CO2 esta de cor verde ou outra qualquer, para se promover o diagnóstico o manipulador injeta o sangue através de uma seringa no duto coletor e o conduz para o interior do frasco onde é misturado com o meio liquido, assim o manipulador entorna o equipamento conduzindo a mistura para o interior do bulbo fazendo com este entre em contato com as placas, sendo que as placas dois meios sólidos promovem o aparecimento de colônias de bactérias ou fungos patogênicos, enquanto a placa indicadora de CO2 mudará sua coloração com a presença do gás.O processo de agitação intermitente produzido pela estufa favorece a multiplicação microbiana e a inversão automatizada e programada dos frascos favorece a semeadura em meios sólidos evitando a necessidade de intervenção de manipulador para esta etapa dentro do laboratório.</description><language>eng ; fre ; por</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; BLASTING ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; DETAILS OF DOMESTIC STOVES OR RANGES, OF GENERAL APPLICATION ; ENZYMOLOGY ; HEATING ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; LIGHTING ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; MECHANICAL ENGINEERING ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; OTHER DOMESTIC STOVES OR RANGES ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; RANGES ; SPIRITS ; TESTING ; VENTILATING ; VINEGAR ; WEAPONS ; WINE</subject><creationdate>2010</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20100603&DB=EPODOC&CC=WO&NR=2010060176A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20100603&DB=EPODOC&CC=WO&NR=2010060176A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MIMICA, IGOR MIMICA</creatorcontrib><title>AUTOMATED INOCULATION MECHANISM WITH INTERMITTENT STIRRING SYSTEM</title><description>Blood culture method for cultivating a patient's blood, and equipment characterised in that, in a single apparatus, an inherent CO2 indicator prepared in a solid agar surface, which allows early diagnosis of seropositivity, is used together with two solid media which allow pathogenic bacteria or fungi to develop without the need to vent the culture bottles. Thus, two bacterial growth indicators (CO2 generation and growth in solid media) are used in the same apparatus, which is formed by a main body in the lower part thereof containing a bottle, from the central part of which an oblique sampling duct projects, said bottle containing a liquid medium and receiving in the upper part thereof a fixed bulb which contains two solid medium plates and one CO2 indicator plate of green colour or of any other colour. In order to facilitate the diagnostic, the manipulator injects blood by means of a syringe into the sampling duct, which leads the blood into the bottle, where the blood is mixed with the liquid medium, then the manipulator turns the apparatus upside down, causing the mixture to fall inside the bulb and come into contact with the plates, whereon two solid media promote the growth of colonies of pathogenic bacteria or fungi, while the CO2 indicator plate changes colour in the presence of the gas. The intermittent stirring process produced in the stove promotes microbial growth and the automated and programmed inversion of the bottles makes inoculation into solid media easier, dispensing with the need for a manipulator in the laboratory for carrying out this step.
L'invention concerne un procédé d'hémoculture pour la mise en culture du sang d'un patient ainsi qu'un équipement associé. L'invention se caractérise en ce qu'on utilise, dans le même équipement, un indicateur de CO2, préparé sur une surface solide avec de l'agar, ce qui permet un diagnostic précoce de positivité, et deux milieux solides permettant le développement de bactéries ou de champignons sans nécessité de soumettre les flacons à une aspiration. Ainsi, deux indicateurs de croissance bactérienne (production de CO2 et multiplication en milieux solides) sont utilisés dans le même équipement, lequel est constitué par un corps principal pourvu inférieurement d'un flacon dont la partie médiane présente un conduit collecteur faisant saillie obliquement. La partie intérieure dudit flacon sert à contenir un milieu liquide, la partie supérieure recevant de manière fixe une ampoule qui renferme deux plaques de milieux solides et une plaque indicatrice de CO2, de couleur verte ou d'une autre couleur quelconque. Pour faciliter le diagnostic, le manipulateur injecte le sang par l'intermédiaire d'une seringue dans le conduit collecteur, le sang étant alors acheminé dans le flacon, où il est mélangé avec le milieu liquide. Le manipulateur retourne ensuite l'équipement, ce qui amène le mélange à entrer dans l'ampoule, où il vient au contact des plaques, les milieux solides favorisant l'apparition de colonies de bactéries ou de champignons pathogènes. Dans le même temps, la plaque indicatrice de CO2 change de couleur en présence du gaz. Un procédé d'agitation intermittente réalisé dans une étuve favorise la multiplication microbienne, et l'inversion automatisée et programmée des flacons favorise l'ensemencement dans des milieux solides, ce qui supprime la nécessité de faire intervenir un manipulateur pour cette étape dans le laboratoire.
Processo de hemocultura para cultivo do sangue de um paciente e equipamento utilizado caracterizado por utilizar no mesmo equipamento um indicador próprio de CO2, preparado numa superfície sólida com agar, o que permite um diagnóstico precoce da positividade, e de dois meios sólidos que permitem sem necessidade de aspirar os frascos o desenvolvimento de bactérias ou fungos patogênicos, assim através de dois indicadores de crescimento bacteriano, (produção de CO2 e multiplicação em meios sólidos) usados no mesmo equipamento esse constituído por um corpo principal que detém inferiormente um frasco que medianamente projeta obliquamente um duto coletor sendo que internamente o dito frasco acondiciona um meio liquido e superiormente recebe de forma fixa um bulbo que internamente detém duas placas de meio sólidos e uma placa indicadora de CO2 esta de cor verde ou outra qualquer, para se promover o diagnóstico o manipulador injeta o sangue através de uma seringa no duto coletor e o conduz para o interior do frasco onde é misturado com o meio liquido, assim o manipulador entorna o equipamento conduzindo a mistura para o interior do bulbo fazendo com este entre em contato com as placas, sendo que as placas dois meios sólidos promovem o aparecimento de colônias de bactérias ou fungos patogênicos, enquanto a placa indicadora de CO2 mudará sua coloração com a presença do gás.O processo de agitação intermitente produzido pela estufa favorece a multiplicação microbiana e a inversão automatizada e programada dos frascos favorece a semeadura em meios sólidos evitando a necessidade de intervenção de manipulador para esta etapa dentro do laboratório.</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>BLASTING</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>DETAILS OF DOMESTIC STOVES OR RANGES, OF GENERAL APPLICATION</subject><subject>ENZYMOLOGY</subject><subject>HEATING</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>LIGHTING</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>MECHANICAL ENGINEERING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>OTHER DOMESTIC STOVES OR RANGES</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>RANGES</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VENTILATING</subject><subject>VINEGAR</subject><subject>WEAPONS</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2010</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHB0DA3x93UMcXVR8PTzdw71cQzx9PdT8HV19nD08wz2VQj3DPEASoW4Bvl6hoS4-oUoBId4BgV5-rkrBEcGh7j68jCwpiXmFKfyQmluBmU31xBnD93Ugvz41OKCxOTUvNSS-HB_IwNDAwMzA0NzM0dDY-JUAQDT3Sx-</recordid><startdate>20100603</startdate><enddate>20100603</enddate><creator>MIMICA, IGOR MIMICA</creator><scope>EVB</scope></search><sort><creationdate>20100603</creationdate><title>AUTOMATED INOCULATION MECHANISM WITH INTERMITTENT STIRRING SYSTEM</title><author>MIMICA, IGOR MIMICA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2010060176A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; por</language><creationdate>2010</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>BLASTING</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>DETAILS OF DOMESTIC STOVES OR RANGES, OF GENERAL APPLICATION</topic><topic>ENZYMOLOGY</topic><topic>HEATING</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>LIGHTING</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>MECHANICAL ENGINEERING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>OTHER DOMESTIC STOVES OR RANGES</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>RANGES</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VENTILATING</topic><topic>VINEGAR</topic><topic>WEAPONS</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MIMICA, IGOR MIMICA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MIMICA, IGOR MIMICA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>AUTOMATED INOCULATION MECHANISM WITH INTERMITTENT STIRRING SYSTEM</title><date>2010-06-03</date><risdate>2010</risdate><abstract>Blood culture method for cultivating a patient's blood, and equipment characterised in that, in a single apparatus, an inherent CO2 indicator prepared in a solid agar surface, which allows early diagnosis of seropositivity, is used together with two solid media which allow pathogenic bacteria or fungi to develop without the need to vent the culture bottles. Thus, two bacterial growth indicators (CO2 generation and growth in solid media) are used in the same apparatus, which is formed by a main body in the lower part thereof containing a bottle, from the central part of which an oblique sampling duct projects, said bottle containing a liquid medium and receiving in the upper part thereof a fixed bulb which contains two solid medium plates and one CO2 indicator plate of green colour or of any other colour. In order to facilitate the diagnostic, the manipulator injects blood by means of a syringe into the sampling duct, which leads the blood into the bottle, where the blood is mixed with the liquid medium, then the manipulator turns the apparatus upside down, causing the mixture to fall inside the bulb and come into contact with the plates, whereon two solid media promote the growth of colonies of pathogenic bacteria or fungi, while the CO2 indicator plate changes colour in the presence of the gas. The intermittent stirring process produced in the stove promotes microbial growth and the automated and programmed inversion of the bottles makes inoculation into solid media easier, dispensing with the need for a manipulator in the laboratory for carrying out this step.
L'invention concerne un procédé d'hémoculture pour la mise en culture du sang d'un patient ainsi qu'un équipement associé. L'invention se caractérise en ce qu'on utilise, dans le même équipement, un indicateur de CO2, préparé sur une surface solide avec de l'agar, ce qui permet un diagnostic précoce de positivité, et deux milieux solides permettant le développement de bactéries ou de champignons sans nécessité de soumettre les flacons à une aspiration. Ainsi, deux indicateurs de croissance bactérienne (production de CO2 et multiplication en milieux solides) sont utilisés dans le même équipement, lequel est constitué par un corps principal pourvu inférieurement d'un flacon dont la partie médiane présente un conduit collecteur faisant saillie obliquement. La partie intérieure dudit flacon sert à contenir un milieu liquide, la partie supérieure recevant de manière fixe une ampoule qui renferme deux plaques de milieux solides et une plaque indicatrice de CO2, de couleur verte ou d'une autre couleur quelconque. Pour faciliter le diagnostic, le manipulateur injecte le sang par l'intermédiaire d'une seringue dans le conduit collecteur, le sang étant alors acheminé dans le flacon, où il est mélangé avec le milieu liquide. Le manipulateur retourne ensuite l'équipement, ce qui amène le mélange à entrer dans l'ampoule, où il vient au contact des plaques, les milieux solides favorisant l'apparition de colonies de bactéries ou de champignons pathogènes. Dans le même temps, la plaque indicatrice de CO2 change de couleur en présence du gaz. Un procédé d'agitation intermittente réalisé dans une étuve favorise la multiplication microbienne, et l'inversion automatisée et programmée des flacons favorise l'ensemencement dans des milieux solides, ce qui supprime la nécessité de faire intervenir un manipulateur pour cette étape dans le laboratoire.
Processo de hemocultura para cultivo do sangue de um paciente e equipamento utilizado caracterizado por utilizar no mesmo equipamento um indicador próprio de CO2, preparado numa superfície sólida com agar, o que permite um diagnóstico precoce da positividade, e de dois meios sólidos que permitem sem necessidade de aspirar os frascos o desenvolvimento de bactérias ou fungos patogênicos, assim através de dois indicadores de crescimento bacteriano, (produção de CO2 e multiplicação em meios sólidos) usados no mesmo equipamento esse constituído por um corpo principal que detém inferiormente um frasco que medianamente projeta obliquamente um duto coletor sendo que internamente o dito frasco acondiciona um meio liquido e superiormente recebe de forma fixa um bulbo que internamente detém duas placas de meio sólidos e uma placa indicadora de CO2 esta de cor verde ou outra qualquer, para se promover o diagnóstico o manipulador injeta o sangue através de uma seringa no duto coletor e o conduz para o interior do frasco onde é misturado com o meio liquido, assim o manipulador entorna o equipamento conduzindo a mistura para o interior do bulbo fazendo com este entre em contato com as placas, sendo que as placas dois meios sólidos promovem o aparecimento de colônias de bactérias ou fungos patogênicos, enquanto a placa indicadora de CO2 mudará sua coloração com a presença do gás.O processo de agitação intermitente produzido pela estufa favorece a multiplicação microbiana e a inversão automatizada e programada dos frascos favorece a semeadura em meios sólidos evitando a necessidade de intervenção de manipulador para esta etapa dentro do laboratório.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY BLASTING CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DETAILS OF DOMESTIC STOVES OR RANGES, OF GENERAL APPLICATION ENZYMOLOGY HEATING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIGHTING MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MECHANICAL ENGINEERING METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING OTHER DOMESTIC STOVES OR RANGES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS RANGES SPIRITS TESTING VENTILATING VINEGAR WEAPONS WINE |
| title | AUTOMATED INOCULATION MECHANISM WITH INTERMITTENT STIRRING SYSTEM |
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