METHOD AND KIT FOR HEPATITIS E VIRUS (HEV) DETECTION

In the present invention there is described a combination of primers and a method for molecular detection of the different circulating strains of the hepatitis E virus (HEV) in humans and animal reservoirs distributed geographically throughout the world. Amplification of the HEV genome is realised b...

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Hauptverfasser:	FOGEDA MORENO, MARTA, ROYUELA CASAMAYOR, JESUS ENRIQUE, POZO SANCHEZ, FRANCISCO, ECHEVARRIA MAYO, JOSE MANUEL, AVELLON CALVO, ANA
Format:	Patent
Sprache:	eng ; fre ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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description	In the present invention there is described a combination of primers and a method for molecular detection of the different circulating strains of the hepatitis E virus (HEV) in humans and animal reservoirs distributed geographically throughout the world. Amplification of the HEV genome is realised by nested RT-PCRs wherein the primers employed amplify two fragments of the ORF1 (Open Reading Frame) and ORF2 regions of the genome of this virus. Furthermore the present invention includes a kit including the combination of the aforementioned primers and other components. En la presente invención se describe una combinación de cebadores y un método para la detección molecular de las diferentes cepas circulantes del virus de la hepatitis E (VHE) en humanos y reservorios animales distribuidas geográficamente por todo el mundo. La amplificación del genoma del VHE se realiza mediante RT-PCRs anidadas donde los cebadores empleados amplifican dos fragmentos de las regiones ORF1 (Open Reading Frame) y ORF2 del genoma de este virus. Además, la presente invención incluye un kit con la combinación de los cebadores mencionados y otros componentes. La présente invention concerne une combinaison d'amorces et une méthode de détection moléculaire des différentes souches en circulation du virus de l'hépatite E (VHE) chez des humains et dans des réservoirs animaux lesquelles sont réparties géographiquement dans le monde entier. L'amplification du génome du VHE s'effectue par RT-PCR imbriquées au cours desquelles les amorces utilisées amplifient deux fragments des régions ORF1 (Open Reading Frame) et ORF2 du génome de ce virus. En outre, la présente invention concerne une trousse comprenant la combinaison des amorces susmentionnées et d'autres composants.
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Amplification of the HEV genome is realised by nested RT-PCRs wherein the primers employed amplify two fragments of the ORF1 (Open Reading Frame) and ORF2 regions of the genome of this virus. Furthermore the present invention includes a kit including the combination of the aforementioned primers and other components. En la presente invención se describe una combinación de cebadores y un método para la detección molecular de las diferentes cepas circulantes del virus de la hepatitis E (VHE) en humanos y reservorios animales distribuidas geográficamente por todo el mundo. La amplificación del genoma del VHE se realiza mediante RT-PCRs anidadas donde los cebadores empleados amplifican dos fragmentos de las regiones ORF1 (Open Reading Frame) y ORF2 del genoma de este virus. Además, la presente invención incluye un kit con la combinación de los cebadores mencionados y otros componentes. La présente invention concerne une combinaison d'amorces et une méthode de détection moléculaire des différentes souches en circulation du virus de l'hépatite E (VHE) chez des humains et dans des réservoirs animaux lesquelles sont réparties géographiquement dans le monde entier. L'amplification du génome du VHE s'effectue par RT-PCR imbriquées au cours desquelles les amorces utilisées amplifient deux fragments des régions ORF1 (Open Reading Frame) et ORF2 du génome de ce virus. 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Amplification of the HEV genome is realised by nested RT-PCRs wherein the primers employed amplify two fragments of the ORF1 (Open Reading Frame) and ORF2 regions of the genome of this virus. Furthermore the present invention includes a kit including the combination of the aforementioned primers and other components. En la presente invención se describe una combinación de cebadores y un método para la detección molecular de las diferentes cepas circulantes del virus de la hepatitis E (VHE) en humanos y reservorios animales distribuidas geográficamente por todo el mundo. La amplificación del genoma del VHE se realiza mediante RT-PCRs anidadas donde los cebadores empleados amplifican dos fragmentos de las regiones ORF1 (Open Reading Frame) y ORF2 del genoma de este virus. Además, la presente invención incluye un kit con la combinación de los cebadores mencionados y otros componentes. La présente invention concerne une combinaison d'amorces et une méthode de détection moléculaire des différentes souches en circulation du virus de l'hépatite E (VHE) chez des humains et dans des réservoirs animaux lesquelles sont réparties géographiquement dans le monde entier. L'amplification du génome du VHE s'effectue par RT-PCR imbriquées au cours desquelles les amorces utilisées amplifient deux fragments des régions ORF1 (Open Reading Frame) et ORF2 du génome de ce virus. 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Amplification of the HEV genome is realised by nested RT-PCRs wherein the primers employed amplify two fragments of the ORF1 (Open Reading Frame) and ORF2 regions of the genome of this virus. Furthermore the present invention includes a kit including the combination of the aforementioned primers and other components. En la presente invención se describe una combinación de cebadores y un método para la detección molecular de las diferentes cepas circulantes del virus de la hepatitis E (VHE) en humanos y reservorios animales distribuidas geográficamente por todo el mundo. La amplificación del genoma del VHE se realiza mediante RT-PCRs anidadas donde los cebadores empleados amplifican dos fragmentos de las regiones ORF1 (Open Reading Frame) y ORF2 del genoma de este virus. Además, la presente invención incluye un kit con la combinación de los cebadores mencionados y otros componentes. La présente invention concerne une combinaison d'amorces et une méthode de détection moléculaire des différentes souches en circulation du virus de l'hépatite E (VHE) chez des humains et dans des réservoirs animaux lesquelles sont réparties géographiquement dans le monde entier. L'amplification du génome du VHE s'effectue par RT-PCR imbriquées au cours desquelles les amorces utilisées amplifient deux fragments des régions ORF1 (Open Reading Frame) et ORF2 du génome de ce virus. En outre, la présente invention concerne une trousse comprenant la combinaison des amorces susmentionnées et d'autres composants.</abstract><oa>free_for_read</oa></addata></record>
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subjects	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
title	METHOD AND KIT FOR HEPATITIS E VIRUS (HEV) DETECTION
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