METHOD FOR SIMULTANEOUSLY DETECTING A PLURALITY OF RNA SEQUENCES IN A BIOLOGICAL SAMPLE
The invention relates to molecular biology, biotechnology, genetic engineering and to medicine and provides a multipurpose method for simultaneously detecting different RNAs in tested samples, including diagnosis purposes during mass analysis of viral and viroidal infections of organisms. The invent...
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| creator | CHIRKOV, SERGEI NIKOLAEVICH ZINOVKIN, ROMAN ALEXEEVICH ATABEKOV, IOSIF GRIGORIEVICH DRYGIN, YURY FEDOROVICH KONDAKOVA, OLGA ALEXANDROVNA KISELEVA, VALENTINA IVANOVNA |
| description | The invention relates to molecular biology, biotechnology, genetic engineering and to medicine and provides a multipurpose method for simultaneously detecting different RNAs in tested samples, including diagnosis purposes during mass analysis of viral and viroidal infections of organisms. The inventive method involves separating a total RNA from a sample, marking the RNA with a diene-platinum mark, crossbreeding the marked RNA with a microchip in the form a substrate with DNA-probes which are immobilised thereon and are specific to the above-mentioned RNA sequences and subsequently detecting the thus formed specific heteroduplexes with the aid of DNA diene-platinum antibodies. A microchip for simultaneously detecting seven viral and viroidal potato infections which are contained in the tested sample and are more economically damaging for potato growing in Russia is also disclosed. The inventive technics for molecular detection of multiple infections makes it possible to substantially accelerate the diagnostic analysis of combined viral infections of different organisms in the case of visually ambiguously identified epiphytotics and epyzootics, during a quarantine and for certification of agricultural plants and animals.
L'invention concerne les domaines de biologie, biotechnologie, génie génétique et médecine et plus particulièrement un procédé universel de détection de différents ARN dans les échantillons analysés, y compris à des fins diagnostiques lors d'analyses en masse d'infections virales ou viroïdes d'organismes vivants. Le procédé prévoit l'extraction de l'ARN global à partir d'un échantillon, le marquage de l'ARN au moyen d'une étiquette diène-platine, l'hybridation de l'ARN étiqueté avec une micropuce qui se présente comme un substrat sur lequel sont immobilisées des sondes ARN spécifiques vis-à-vis des séquences ARN d'intérêt, puis la détection d'hétéroduplex spécifiques au moyen d'anticorps pour le diène-platine d'ADN. L'invention porte sur une micropuce diagnostique destinée à la détermination simultanée de sept infections virales ou viroïdes des pommes de terre dans un échantillon analysé, qui a un impact négatif considérable sur la culture de la pomme de terre en Russie. Selon l'invention, cette technologie de diagnostic moléculaire d'infections virales multiples permet d'accélérer radicalement l'analyse diagnostique d'infections virales mixtes de différents organismes en cas d'épizooties épiphytoties dont le diagnostic visuel apporte des r |
| format | Patent |
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L'invention concerne les domaines de biologie, biotechnologie, génie génétique et médecine et plus particulièrement un procédé universel de détection de différents ARN dans les échantillons analysés, y compris à des fins diagnostiques lors d'analyses en masse d'infections virales ou viroïdes d'organismes vivants. Le procédé prévoit l'extraction de l'ARN global à partir d'un échantillon, le marquage de l'ARN au moyen d'une étiquette diène-platine, l'hybridation de l'ARN étiqueté avec une micropuce qui se présente comme un substrat sur lequel sont immobilisées des sondes ARN spécifiques vis-à-vis des séquences ARN d'intérêt, puis la détection d'hétéroduplex spécifiques au moyen d'anticorps pour le diène-platine d'ADN. L'invention porte sur une micropuce diagnostique destinée à la détermination simultanée de sept infections virales ou viroïdes des pommes de terre dans un échantillon analysé, qui a un impact négatif considérable sur la culture de la pomme de terre en Russie. Selon l'invention, cette technologie de diagnostic moléculaire d'infections virales multiples permet d'accélérer radicalement l'analyse diagnostique d'infections virales mixtes de différents organismes en cas d'épizooties épiphytoties dont le diagnostic visuel apporte des résultats controversés, de déclaration de quarantaine, à des fins de certification de plantes ou animaux agricoles, etc.</description><language>eng ; fre ; rus</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2009</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20091008&DB=EPODOC&CC=WO&NR=2009123494A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20091008&DB=EPODOC&CC=WO&NR=2009123494A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHIRKOV, SERGEI NIKOLAEVICH</creatorcontrib><creatorcontrib>ZINOVKIN, ROMAN ALEXEEVICH</creatorcontrib><creatorcontrib>ATABEKOV, IOSIF GRIGORIEVICH</creatorcontrib><creatorcontrib>DRYGIN, YURY FEDOROVICH</creatorcontrib><creatorcontrib>KONDAKOVA, OLGA ALEXANDROVNA</creatorcontrib><creatorcontrib>KISELEVA, VALENTINA IVANOVNA</creatorcontrib><title>METHOD FOR SIMULTANEOUSLY DETECTING A PLURALITY OF RNA SEQUENCES IN A BIOLOGICAL SAMPLE</title><description>The invention relates to molecular biology, biotechnology, genetic engineering and to medicine and provides a multipurpose method for simultaneously detecting different RNAs in tested samples, including diagnosis purposes during mass analysis of viral and viroidal infections of organisms. The inventive method involves separating a total RNA from a sample, marking the RNA with a diene-platinum mark, crossbreeding the marked RNA with a microchip in the form a substrate with DNA-probes which are immobilised thereon and are specific to the above-mentioned RNA sequences and subsequently detecting the thus formed specific heteroduplexes with the aid of DNA diene-platinum antibodies. A microchip for simultaneously detecting seven viral and viroidal potato infections which are contained in the tested sample and are more economically damaging for potato growing in Russia is also disclosed. The inventive technics for molecular detection of multiple infections makes it possible to substantially accelerate the diagnostic analysis of combined viral infections of different organisms in the case of visually ambiguously identified epiphytotics and epyzootics, during a quarantine and for certification of agricultural plants and animals.
L'invention concerne les domaines de biologie, biotechnologie, génie génétique et médecine et plus particulièrement un procédé universel de détection de différents ARN dans les échantillons analysés, y compris à des fins diagnostiques lors d'analyses en masse d'infections virales ou viroïdes d'organismes vivants. Le procédé prévoit l'extraction de l'ARN global à partir d'un échantillon, le marquage de l'ARN au moyen d'une étiquette diène-platine, l'hybridation de l'ARN étiqueté avec une micropuce qui se présente comme un substrat sur lequel sont immobilisées des sondes ARN spécifiques vis-à-vis des séquences ARN d'intérêt, puis la détection d'hétéroduplex spécifiques au moyen d'anticorps pour le diène-platine d'ADN. L'invention porte sur une micropuce diagnostique destinée à la détermination simultanée de sept infections virales ou viroïdes des pommes de terre dans un échantillon analysé, qui a un impact négatif considérable sur la culture de la pomme de terre en Russie. Selon l'invention, cette technologie de diagnostic moléculaire d'infections virales multiples permet d'accélérer radicalement l'analyse diagnostique d'infections virales mixtes de différents organismes en cas d'épizooties épiphytoties dont le diagnostic visuel apporte des résultats controversés, de déclaration de quarantaine, à des fins de certification de plantes ou animaux agricoles, etc.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2009</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyj0OgkAQQGEaC6PeYRJrE_4aymEZYJNhB9ndECpCzFoZJcH7RwsPYPWK7-2jsSPXSgW1DGB159mhIfGWJ6jIkXLaNIDQsx-QtZtAahgMgqWrJ6PIgjZfL7WwNFohg8WuZzpGu_vy2MLp10N0rsmp9hLW1xy2dbmFZ3jPo6RxXCRplhc5Jtl_1weJiDHX</recordid><startdate>20091008</startdate><enddate>20091008</enddate><creator>CHIRKOV, SERGEI NIKOLAEVICH</creator><creator>ZINOVKIN, ROMAN ALEXEEVICH</creator><creator>ATABEKOV, IOSIF GRIGORIEVICH</creator><creator>DRYGIN, YURY FEDOROVICH</creator><creator>KONDAKOVA, OLGA ALEXANDROVNA</creator><creator>KISELEVA, VALENTINA IVANOVNA</creator><scope>EVB</scope></search><sort><creationdate>20091008</creationdate><title>METHOD FOR SIMULTANEOUSLY DETECTING A PLURALITY OF RNA SEQUENCES IN A BIOLOGICAL SAMPLE</title><author>CHIRKOV, SERGEI NIKOLAEVICH ; ZINOVKIN, ROMAN ALEXEEVICH ; ATABEKOV, IOSIF GRIGORIEVICH ; DRYGIN, YURY FEDOROVICH ; KONDAKOVA, OLGA ALEXANDROVNA ; KISELEVA, VALENTINA IVANOVNA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2009123494A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; rus</language><creationdate>2009</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CHIRKOV, SERGEI NIKOLAEVICH</creatorcontrib><creatorcontrib>ZINOVKIN, ROMAN ALEXEEVICH</creatorcontrib><creatorcontrib>ATABEKOV, IOSIF GRIGORIEVICH</creatorcontrib><creatorcontrib>DRYGIN, YURY FEDOROVICH</creatorcontrib><creatorcontrib>KONDAKOVA, OLGA ALEXANDROVNA</creatorcontrib><creatorcontrib>KISELEVA, VALENTINA IVANOVNA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CHIRKOV, SERGEI NIKOLAEVICH</au><au>ZINOVKIN, ROMAN ALEXEEVICH</au><au>ATABEKOV, IOSIF GRIGORIEVICH</au><au>DRYGIN, YURY FEDOROVICH</au><au>KONDAKOVA, OLGA ALEXANDROVNA</au><au>KISELEVA, VALENTINA IVANOVNA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR SIMULTANEOUSLY DETECTING A PLURALITY OF RNA SEQUENCES IN A BIOLOGICAL SAMPLE</title><date>2009-10-08</date><risdate>2009</risdate><abstract>The invention relates to molecular biology, biotechnology, genetic engineering and to medicine and provides a multipurpose method for simultaneously detecting different RNAs in tested samples, including diagnosis purposes during mass analysis of viral and viroidal infections of organisms. The inventive method involves separating a total RNA from a sample, marking the RNA with a diene-platinum mark, crossbreeding the marked RNA with a microchip in the form a substrate with DNA-probes which are immobilised thereon and are specific to the above-mentioned RNA sequences and subsequently detecting the thus formed specific heteroduplexes with the aid of DNA diene-platinum antibodies. A microchip for simultaneously detecting seven viral and viroidal potato infections which are contained in the tested sample and are more economically damaging for potato growing in Russia is also disclosed. The inventive technics for molecular detection of multiple infections makes it possible to substantially accelerate the diagnostic analysis of combined viral infections of different organisms in the case of visually ambiguously identified epiphytotics and epyzootics, during a quarantine and for certification of agricultural plants and animals.
L'invention concerne les domaines de biologie, biotechnologie, génie génétique et médecine et plus particulièrement un procédé universel de détection de différents ARN dans les échantillons analysés, y compris à des fins diagnostiques lors d'analyses en masse d'infections virales ou viroïdes d'organismes vivants. Le procédé prévoit l'extraction de l'ARN global à partir d'un échantillon, le marquage de l'ARN au moyen d'une étiquette diène-platine, l'hybridation de l'ARN étiqueté avec une micropuce qui se présente comme un substrat sur lequel sont immobilisées des sondes ARN spécifiques vis-à-vis des séquences ARN d'intérêt, puis la détection d'hétéroduplex spécifiques au moyen d'anticorps pour le diène-platine d'ADN. L'invention porte sur une micropuce diagnostique destinée à la détermination simultanée de sept infections virales ou viroïdes des pommes de terre dans un échantillon analysé, qui a un impact négatif considérable sur la culture de la pomme de terre en Russie. Selon l'invention, cette technologie de diagnostic moléculaire d'infections virales multiples permet d'accélérer radicalement l'analyse diagnostique d'infections virales mixtes de différents organismes en cas d'épizooties épiphytoties dont le diagnostic visuel apporte des résultats controversés, de déclaration de quarantaine, à des fins de certification de plantes ou animaux agricoles, etc.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | METHOD FOR SIMULTANEOUSLY DETECTING A PLURALITY OF RNA SEQUENCES IN A BIOLOGICAL SAMPLE |
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