AN IMPROVED NUCLEIC ACID PURIFICATION METHOD
The invention provides an modified method for the separation of nucleic acids from cells, comprising: generating an aqueous solution containing the nucleic acid by lysing the cells with a lysis solution including SDS and salt; and separating the nucleic acids of interest from other cellular componen...
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| creator | KHAN, MANZER GABRIEL, ANABELA, BRITO DAR, MUBASHER |
| description | The invention provides an modified method for the separation of nucleic acids from cells, comprising: generating an aqueous solution containing the nucleic acid by lysing the cells with a lysis solution including SDS and salt; and separating the nucleic acids of interest from other cellular components. The improvement includes adding a non-ionic detergent in the lysis solution such that SDS is not precipitated and no heating of the solution is required prior to cellular lysis. The preferred non-ionic detergents are the polysorbate family of compound, including Tween 20. Also disclosed are composition and kit for performing the modified method.
L'invention concerne un procédé modifié pour la séparation des acides nucléiques à partir de cellules, comprenant les étapes consistant à : générer une solution aqueuse contenant l'acide nucléique en lysant les cellules avec une solution de lyse comprenant du SDS et du sel; et séparer les acides nucléiques intéressants des autres composants cellulaires. L'amélioration comprend l'ajout d'un détergent non ionique dans la solution de lyse de telle sorte que le SDS ne précipite pas, et qu'aucun chauffage de la solution ne soit nécessaire avant une lyse cellulaire. Les détergents non ioniques préférés sont de la famille du composé de polysorbate, y compris le Tween 20. L'invention concerne également un composé et un kit pour mettre en oevre le procédé modifié. |
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L'invention concerne un procédé modifié pour la séparation des acides nucléiques à partir de cellules, comprenant les étapes consistant à : générer une solution aqueuse contenant l'acide nucléique en lysant les cellules avec une solution de lyse comprenant du SDS et du sel; et séparer les acides nucléiques intéressants des autres composants cellulaires. L'amélioration comprend l'ajout d'un détergent non ionique dans la solution de lyse de telle sorte que le SDS ne précipite pas, et qu'aucun chauffage de la solution ne soit nécessaire avant une lyse cellulaire. Les détergents non ioniques préférés sont de la famille du composé de polysorbate, y compris le Tween 20. L'invention concerne également un composé et un kit pour mettre en oevre le procédé modifié.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DERIVATIVES THEREOF ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ORGANIC CHEMISTRY ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; SUGARS ; VINEGAR ; WINE</subject><creationdate>2009</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20090205&DB=EPODOC&CC=WO&NR=2009018034A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,782,887,25571,76555</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20090205&DB=EPODOC&CC=WO&NR=2009018034A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KHAN, MANZER</creatorcontrib><creatorcontrib>GABRIEL, ANABELA, BRITO</creatorcontrib><creatorcontrib>DAR, MUBASHER</creatorcontrib><title>AN IMPROVED NUCLEIC ACID PURIFICATION METHOD</title><description>The invention provides an modified method for the separation of nucleic acids from cells, comprising: generating an aqueous solution containing the nucleic acid by lysing the cells with a lysis solution including SDS and salt; and separating the nucleic acids of interest from other cellular components. The improvement includes adding a non-ionic detergent in the lysis solution such that SDS is not precipitated and no heating of the solution is required prior to cellular lysis. The preferred non-ionic detergents are the polysorbate family of compound, including Tween 20. Also disclosed are composition and kit for performing the modified method.
L'invention concerne un procédé modifié pour la séparation des acides nucléiques à partir de cellules, comprenant les étapes consistant à : générer une solution aqueuse contenant l'acide nucléique en lysant les cellules avec une solution de lyse comprenant du SDS et du sel; et séparer les acides nucléiques intéressants des autres composants cellulaires. L'amélioration comprend l'ajout d'un détergent non ionique dans la solution de lyse de telle sorte que le SDS ne précipite pas, et qu'aucun chauffage de la solution ne soit nécessaire avant une lyse cellulaire. Les détergents non ioniques préférés sont de la famille du composé de polysorbate, y compris le Tween 20. L'invention concerne également un composé et un kit pour mettre en oevre le procédé modifié.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DERIVATIVES THEREOF</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOSIDES</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC CHEMISTRY</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>SUGARS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2009</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNBx9FPw9A0I8g9zdVHwC3X2cfV0VnB09nRRCAgN8nTzdHYM8fT3U_B1DfHwd-FhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHh_kYGBpYGhhYGxiaOhsbEqQIAG5sl8A</recordid><startdate>20090205</startdate><enddate>20090205</enddate><creator>KHAN, MANZER</creator><creator>GABRIEL, ANABELA, BRITO</creator><creator>DAR, MUBASHER</creator><scope>EVB</scope></search><sort><creationdate>20090205</creationdate><title>AN IMPROVED NUCLEIC ACID PURIFICATION METHOD</title><author>KHAN, MANZER ; GABRIEL, ANABELA, BRITO ; DAR, MUBASHER</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2009018034A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2009</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DERIVATIVES THEREOF</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOSIDES</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC CHEMISTRY</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>SUGARS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KHAN, MANZER</creatorcontrib><creatorcontrib>GABRIEL, ANABELA, BRITO</creatorcontrib><creatorcontrib>DAR, MUBASHER</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KHAN, MANZER</au><au>GABRIEL, ANABELA, BRITO</au><au>DAR, MUBASHER</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>AN IMPROVED NUCLEIC ACID PURIFICATION METHOD</title><date>2009-02-05</date><risdate>2009</risdate><abstract>The invention provides an modified method for the separation of nucleic acids from cells, comprising: generating an aqueous solution containing the nucleic acid by lysing the cells with a lysis solution including SDS and salt; and separating the nucleic acids of interest from other cellular components. The improvement includes adding a non-ionic detergent in the lysis solution such that SDS is not precipitated and no heating of the solution is required prior to cellular lysis. The preferred non-ionic detergents are the polysorbate family of compound, including Tween 20. Also disclosed are composition and kit for performing the modified method.
L'invention concerne un procédé modifié pour la séparation des acides nucléiques à partir de cellules, comprenant les étapes consistant à : générer une solution aqueuse contenant l'acide nucléique en lysant les cellules avec une solution de lyse comprenant du SDS et du sel; et séparer les acides nucléiques intéressants des autres composants cellulaires. L'amélioration comprend l'ajout d'un détergent non ionique dans la solution de lyse de telle sorte que le SDS ne précipite pas, et qu'aucun chauffage de la solution ne soit nécessaire avant une lyse cellulaire. Les détergents non ioniques préférés sont de la famille du composé de polysorbate, y compris le Tween 20. L'invention concerne également un composé et un kit pour mettre en oevre le procédé modifié.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE |
| title | AN IMPROVED NUCLEIC ACID PURIFICATION METHOD |
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