YEAST PLATFORM CONSTRUCTION AND SCREENING METHODS
The present invention relates to a cell which is suitable for screening a candidate agent as being an inhibitor of the metabolism of tryptophan to NAD+ and/or a modulator of NAD+ levels, which cell comprises functional genes of a pathway enabling the metabolism of tryptophan to NAD+ and wherein the...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; fre |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | VIEIRA, HELENA MARGARIDA MOREIRA DE OLIVEIRA ROCA, CHRISTOPHE FRANCOIS AIME RODRIGUES, CATIA SANTANA REVERENDO CALADO, PATRICIA RAMALHETE MENDES DA SILVA DOS SANTOS, ALEXANDRA MARIA BARROS PINHEIRO, RICARDO FILIPE ANTUNES SAM, JOHANNES CEREJO, MARTA ISABEL HEITOR CHATTERJEE, SUKALYAN SOUSA, JOSE MANUEL BERNARDO |
| description | The present invention relates to a cell which is suitable for screening a candidate agent as being an inhibitor of the metabolism of tryptophan to NAD+ and/or a modulator of NAD+ levels, which cell comprises functional genes of a pathway enabling the metabolism of tryptophan to NAD+ and wherein the cell includes a copy of an exogenous gene of said pathway, from the same or different species as the cell, which exogenous gene is under the control of an inducible or constitutive promoter and wherein any endogenous copy of the gene having the same function as the exogenous gene is a non functioning gene. The present invention also relates to populations of such cells and to methods of screening candidate agents with such cells.
L'invention concerne une cellule qui permet de cribler un agent candidat inhibiteur du métabolisme de la conversion du tryptophan en NAD+ et/ou modulateur des niveaux de NAD+, laquelle cellule comprend des gènes fonctionnels d'une voie permettant le métabolisme de la conversion du tryptophan en NAD+ et comprend une copie d'un gène exogène de ladite voie, issu d'une espèce semblable à celle de la cellule ou différente de celle-ci. La cellule exogène est sous le contrôle d'un promoteur inductible ou constitutif, et toute copie endogène du gène ayant la même fonction que le gène exogène est un gène non fonctionnel. L'invention se rapporte également à des populations de cellules et à des procédés permettant de cribler les agents candidats à l'aide desdites cellules. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_WO2008075991A2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>WO2008075991A2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_WO2008075991A23</originalsourceid><addsrcrecordid>eNrjZDCMdHUMDlEI8HEMcfMP8lVw9vcLDgkKdQ7x9PdTcPRzUQh2DnJ19fP0c1fwdQ3x8HcJ5mFgTUvMKU7lhdLcDMpuriHOHrqpBfnxqcUFicmpeakl8eH-RgYGFgbmppaWho5GxsSpAgATGCfX</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>YEAST PLATFORM CONSTRUCTION AND SCREENING METHODS</title><source>esp@cenet</source><creator>VIEIRA, HELENA MARGARIDA MOREIRA DE OLIVEIRA ; ROCA, CHRISTOPHE FRANCOIS AIME ; RODRIGUES, CATIA SANTANA REVERENDO ; CALADO, PATRICIA RAMALHETE MENDES DA SILVA ; DOS SANTOS, ALEXANDRA MARIA BARROS ; PINHEIRO, RICARDO FILIPE ANTUNES ; SAM, JOHANNES ; CEREJO, MARTA ISABEL HEITOR ; CHATTERJEE, SUKALYAN ; SOUSA, JOSE MANUEL BERNARDO</creator><creatorcontrib>VIEIRA, HELENA MARGARIDA MOREIRA DE OLIVEIRA ; ROCA, CHRISTOPHE FRANCOIS AIME ; RODRIGUES, CATIA SANTANA REVERENDO ; CALADO, PATRICIA RAMALHETE MENDES DA SILVA ; DOS SANTOS, ALEXANDRA MARIA BARROS ; PINHEIRO, RICARDO FILIPE ANTUNES ; SAM, JOHANNES ; CEREJO, MARTA ISABEL HEITOR ; CHATTERJEE, SUKALYAN ; SOUSA, JOSE MANUEL BERNARDO</creatorcontrib><description>The present invention relates to a cell which is suitable for screening a candidate agent as being an inhibitor of the metabolism of tryptophan to NAD+ and/or a modulator of NAD+ levels, which cell comprises functional genes of a pathway enabling the metabolism of tryptophan to NAD+ and wherein the cell includes a copy of an exogenous gene of said pathway, from the same or different species as the cell, which exogenous gene is under the control of an inducible or constitutive promoter and wherein any endogenous copy of the gene having the same function as the exogenous gene is a non functioning gene. The present invention also relates to populations of such cells and to methods of screening candidate agents with such cells.
L'invention concerne une cellule qui permet de cribler un agent candidat inhibiteur du métabolisme de la conversion du tryptophan en NAD+ et/ou modulateur des niveaux de NAD+, laquelle cellule comprend des gènes fonctionnels d'une voie permettant le métabolisme de la conversion du tryptophan en NAD+ et comprend une copie d'un gène exogène de ladite voie, issu d'une espèce semblable à celle de la cellule ou différente de celle-ci. La cellule exogène est sous le contrôle d'un promoteur inductible ou constitutif, et toute copie endogène du gène ayant la même fonction que le gène exogène est un gène non fonctionnel. L'invention se rapporte également à des populations de cellules et à des procédés permettant de cribler les agents candidats à l'aide desdites cellules.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2008</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20080626&DB=EPODOC&CC=WO&NR=2008075991A2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20080626&DB=EPODOC&CC=WO&NR=2008075991A2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>VIEIRA, HELENA MARGARIDA MOREIRA DE OLIVEIRA</creatorcontrib><creatorcontrib>ROCA, CHRISTOPHE FRANCOIS AIME</creatorcontrib><creatorcontrib>RODRIGUES, CATIA SANTANA REVERENDO</creatorcontrib><creatorcontrib>CALADO, PATRICIA RAMALHETE MENDES DA SILVA</creatorcontrib><creatorcontrib>DOS SANTOS, ALEXANDRA MARIA BARROS</creatorcontrib><creatorcontrib>PINHEIRO, RICARDO FILIPE ANTUNES</creatorcontrib><creatorcontrib>SAM, JOHANNES</creatorcontrib><creatorcontrib>CEREJO, MARTA ISABEL HEITOR</creatorcontrib><creatorcontrib>CHATTERJEE, SUKALYAN</creatorcontrib><creatorcontrib>SOUSA, JOSE MANUEL BERNARDO</creatorcontrib><title>YEAST PLATFORM CONSTRUCTION AND SCREENING METHODS</title><description>The present invention relates to a cell which is suitable for screening a candidate agent as being an inhibitor of the metabolism of tryptophan to NAD+ and/or a modulator of NAD+ levels, which cell comprises functional genes of a pathway enabling the metabolism of tryptophan to NAD+ and wherein the cell includes a copy of an exogenous gene of said pathway, from the same or different species as the cell, which exogenous gene is under the control of an inducible or constitutive promoter and wherein any endogenous copy of the gene having the same function as the exogenous gene is a non functioning gene. The present invention also relates to populations of such cells and to methods of screening candidate agents with such cells.
L'invention concerne une cellule qui permet de cribler un agent candidat inhibiteur du métabolisme de la conversion du tryptophan en NAD+ et/ou modulateur des niveaux de NAD+, laquelle cellule comprend des gènes fonctionnels d'une voie permettant le métabolisme de la conversion du tryptophan en NAD+ et comprend une copie d'un gène exogène de ladite voie, issu d'une espèce semblable à celle de la cellule ou différente de celle-ci. La cellule exogène est sous le contrôle d'un promoteur inductible ou constitutif, et toute copie endogène du gène ayant la même fonction que le gène exogène est un gène non fonctionnel. L'invention se rapporte également à des populations de cellules et à des procédés permettant de cribler les agents candidats à l'aide desdites cellules.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2008</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDCMdHUMDlEI8HEMcfMP8lVw9vcLDgkKdQ7x9PdTcPRzUQh2DnJ19fP0c1fwdQ3x8HcJ5mFgTUvMKU7lhdLcDMpuriHOHrqpBfnxqcUFicmpeakl8eH-RgYGFgbmppaWho5GxsSpAgATGCfX</recordid><startdate>20080626</startdate><enddate>20080626</enddate><creator>VIEIRA, HELENA MARGARIDA MOREIRA DE OLIVEIRA</creator><creator>ROCA, CHRISTOPHE FRANCOIS AIME</creator><creator>RODRIGUES, CATIA SANTANA REVERENDO</creator><creator>CALADO, PATRICIA RAMALHETE MENDES DA SILVA</creator><creator>DOS SANTOS, ALEXANDRA MARIA BARROS</creator><creator>PINHEIRO, RICARDO FILIPE ANTUNES</creator><creator>SAM, JOHANNES</creator><creator>CEREJO, MARTA ISABEL HEITOR</creator><creator>CHATTERJEE, SUKALYAN</creator><creator>SOUSA, JOSE MANUEL BERNARDO</creator><scope>EVB</scope></search><sort><creationdate>20080626</creationdate><title>YEAST PLATFORM CONSTRUCTION AND SCREENING METHODS</title><author>VIEIRA, HELENA MARGARIDA MOREIRA DE OLIVEIRA ; ROCA, CHRISTOPHE FRANCOIS AIME ; RODRIGUES, CATIA SANTANA REVERENDO ; CALADO, PATRICIA RAMALHETE MENDES DA SILVA ; DOS SANTOS, ALEXANDRA MARIA BARROS ; PINHEIRO, RICARDO FILIPE ANTUNES ; SAM, JOHANNES ; CEREJO, MARTA ISABEL HEITOR ; CHATTERJEE, SUKALYAN ; SOUSA, JOSE MANUEL BERNARDO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2008075991A23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2008</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>VIEIRA, HELENA MARGARIDA MOREIRA DE OLIVEIRA</creatorcontrib><creatorcontrib>ROCA, CHRISTOPHE FRANCOIS AIME</creatorcontrib><creatorcontrib>RODRIGUES, CATIA SANTANA REVERENDO</creatorcontrib><creatorcontrib>CALADO, PATRICIA RAMALHETE MENDES DA SILVA</creatorcontrib><creatorcontrib>DOS SANTOS, ALEXANDRA MARIA BARROS</creatorcontrib><creatorcontrib>PINHEIRO, RICARDO FILIPE ANTUNES</creatorcontrib><creatorcontrib>SAM, JOHANNES</creatorcontrib><creatorcontrib>CEREJO, MARTA ISABEL HEITOR</creatorcontrib><creatorcontrib>CHATTERJEE, SUKALYAN</creatorcontrib><creatorcontrib>SOUSA, JOSE MANUEL BERNARDO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>VIEIRA, HELENA MARGARIDA MOREIRA DE OLIVEIRA</au><au>ROCA, CHRISTOPHE FRANCOIS AIME</au><au>RODRIGUES, CATIA SANTANA REVERENDO</au><au>CALADO, PATRICIA RAMALHETE MENDES DA SILVA</au><au>DOS SANTOS, ALEXANDRA MARIA BARROS</au><au>PINHEIRO, RICARDO FILIPE ANTUNES</au><au>SAM, JOHANNES</au><au>CEREJO, MARTA ISABEL HEITOR</au><au>CHATTERJEE, SUKALYAN</au><au>SOUSA, JOSE MANUEL BERNARDO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>YEAST PLATFORM CONSTRUCTION AND SCREENING METHODS</title><date>2008-06-26</date><risdate>2008</risdate><abstract>The present invention relates to a cell which is suitable for screening a candidate agent as being an inhibitor of the metabolism of tryptophan to NAD+ and/or a modulator of NAD+ levels, which cell comprises functional genes of a pathway enabling the metabolism of tryptophan to NAD+ and wherein the cell includes a copy of an exogenous gene of said pathway, from the same or different species as the cell, which exogenous gene is under the control of an inducible or constitutive promoter and wherein any endogenous copy of the gene having the same function as the exogenous gene is a non functioning gene. The present invention also relates to populations of such cells and to methods of screening candidate agents with such cells.
L'invention concerne une cellule qui permet de cribler un agent candidat inhibiteur du métabolisme de la conversion du tryptophan en NAD+ et/ou modulateur des niveaux de NAD+, laquelle cellule comprend des gènes fonctionnels d'une voie permettant le métabolisme de la conversion du tryptophan en NAD+ et comprend une copie d'un gène exogène de ladite voie, issu d'une espèce semblable à celle de la cellule ou différente de celle-ci. La cellule exogène est sous le contrôle d'un promoteur inductible ou constitutif, et toute copie endogène du gène ayant la même fonction que le gène exogène est un gène non fonctionnel. L'invention se rapporte également à des populations de cellules et à des procédés permettant de cribler les agents candidats à l'aide desdites cellules.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; fre |
| recordid | cdi_epo_espacenet_WO2008075991A2 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | YEAST PLATFORM CONSTRUCTION AND SCREENING METHODS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T10%3A30%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=VIEIRA,%20HELENA%20MARGARIDA%20MOREIRA%20DE%20OLIVEIRA&rft.date=2008-06-26&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EWO2008075991A2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |