METHOD OF CLASSIFYING ANTIGEN, METHOD OF IDENTIFYING ANTIGEN, METHOD OF OBTAINING ANTIBODY OR ANTIBODY SET, METHOD OF CONSTRUCTING ANTIBODY PANEL AND ANTIBODY OR ANTIBODY SET AND USE OF THE SAME
It is intended to provide a method whereby a plural number of antibodies against cell surface antigens are quickly classified and to provide a method whereby antigens of the thus classified antibodies are quickly identified. Further, it is intended to provide a method of promoting the utilization of...
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| creator | TSUTSUMI, SUSUMU MATSUDA, KAZUKI UKAI, YOSHINORI AKAHORI, YASUSHI HAYASHI, NOBUHIRO TANAKA, MIHO KUROSAWA, YOSHIKAZU SUMITOMO, MARIKO TAKASAKI, AKIHIKO SUGIMOTO, MOTOTAKA MORITA, MIWA AZUMA, MASACHIKA SUZUKI, KAZUHIRO KUROSAWA, GENE SATOU, NORIKO SHIRAISHI, MAMORU EGUCHI, KEIKO SUGIOKA, ATSUSHI MURAMATSU, CHIHO |
| description | It is intended to provide a method whereby a plural number of antibodies against cell surface antigens are quickly classified and to provide a method whereby antigens of the thus classified antibodies are quickly identified. Further, it is intended to provide a method of promoting the utilization of the useful data obtained by the above methods. Furthermore, it is intended to provide an antibody which is effective in treating or diagnosing cancer. Namely, a method of classifying antibodies which comprises: (1) the step of preparing a plural number of antibodies respectively recognizing cell surface antigens; (2) the step of bringing each of these antibodies into contact with a cell of the same species; (3) the step of analyzing each of the cells having been treated in the step (2) by flow cytometry and thus obtaining data indicating the reactivity of each antibody with its cell surface antigen; and (4) the step of comparing the thus obtained data and classifying the individual antibodies depending on the similarity. A method of identifying antigens which further comprises: (5) the step of selecting one to several antibodies from each antibody group formed in the step (4) and identifying antigens thereof; and (6) on the assumption that antigens of the antibodies belonging to a single antibody group are the same or highly related to one another, making relations between the antigens having been identified in the step (5) and the antibody groups to thereby identify the antigens. An antibody against HER1, an antibody against HER2, an antibody against CD46, an antibody against ITGA3, an antibody against ICAM1, an antibody against ALCAM, an antibody against CD147, an antibody against C1qR, an antibody against CD44, an antibody against CD73, an antibody against EpCAM and an antibody against HGFR, each obtained by using the above methods.
La présente invention concerne un procédé au moyen duquel on peut classifier rapidement un grand nombre d'anticorps par rapport à des antigènes de surface cellulaire et qui constitue un procédé permettant d'identifier rapidement les antigènes desdits anticorps ainsi classifiés. L'invention porte également sur un procédé qui favorise l'utilisation des données utiles obtenues au moyen des procédés présentés ci-avant. On cherche également à utiliser un anticorps qui est efficace dans le traitement ou le diagnostic du cancer. Un procédé de classification des anticorps comprend les étapes suivantes: (1) la préparation d'une pluralité |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_WO2008007648A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>WO2008007648A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_WO2008007648A13</originalsourceid><addsrcrecordid>eNrjZDjk6xri4e-i4O-m4OzjGBzs6Rbp6eeu4OgX4unu6qejgJD2dHEFCuKU9ncKcfT0g0k6-btEKvgHIdjBriHIqp39_YJDgkKdQ1A0BDj6ufoAuS44zQBLhga7gswI8XBVCHb0deVhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHh_kYGBhYGBuZmJhaOhsbEqQIA7wdOVg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>METHOD OF CLASSIFYING ANTIGEN, METHOD OF IDENTIFYING ANTIGEN, METHOD OF OBTAINING ANTIBODY OR ANTIBODY SET, METHOD OF CONSTRUCTING ANTIBODY PANEL AND ANTIBODY OR ANTIBODY SET AND USE OF THE SAME</title><source>esp@cenet</source><creator>TSUTSUMI, SUSUMU ; MATSUDA, KAZUKI ; UKAI, YOSHINORI ; AKAHORI, YASUSHI ; HAYASHI, NOBUHIRO ; TANAKA, MIHO ; KUROSAWA, YOSHIKAZU ; SUMITOMO, MARIKO ; TAKASAKI, AKIHIKO ; SUGIMOTO, MOTOTAKA ; MORITA, MIWA ; AZUMA, MASACHIKA ; SUZUKI, KAZUHIRO ; KUROSAWA, GENE ; SATOU, NORIKO ; SHIRAISHI, MAMORU ; EGUCHI, KEIKO ; SUGIOKA, ATSUSHI ; MURAMATSU, CHIHO</creator><creatorcontrib>TSUTSUMI, SUSUMU ; MATSUDA, KAZUKI ; UKAI, YOSHINORI ; AKAHORI, YASUSHI ; HAYASHI, NOBUHIRO ; TANAKA, MIHO ; KUROSAWA, YOSHIKAZU ; SUMITOMO, MARIKO ; TAKASAKI, AKIHIKO ; SUGIMOTO, MOTOTAKA ; MORITA, MIWA ; AZUMA, MASACHIKA ; SUZUKI, KAZUHIRO ; KUROSAWA, GENE ; SATOU, NORIKO ; SHIRAISHI, MAMORU ; EGUCHI, KEIKO ; SUGIOKA, ATSUSHI ; MURAMATSU, CHIHO</creatorcontrib><description>It is intended to provide a method whereby a plural number of antibodies against cell surface antigens are quickly classified and to provide a method whereby antigens of the thus classified antibodies are quickly identified. Further, it is intended to provide a method of promoting the utilization of the useful data obtained by the above methods. Furthermore, it is intended to provide an antibody which is effective in treating or diagnosing cancer. Namely, a method of classifying antibodies which comprises: (1) the step of preparing a plural number of antibodies respectively recognizing cell surface antigens; (2) the step of bringing each of these antibodies into contact with a cell of the same species; (3) the step of analyzing each of the cells having been treated in the step (2) by flow cytometry and thus obtaining data indicating the reactivity of each antibody with its cell surface antigen; and (4) the step of comparing the thus obtained data and classifying the individual antibodies depending on the similarity. A method of identifying antigens which further comprises: (5) the step of selecting one to several antibodies from each antibody group formed in the step (4) and identifying antigens thereof; and (6) on the assumption that antigens of the antibodies belonging to a single antibody group are the same or highly related to one another, making relations between the antigens having been identified in the step (5) and the antibody groups to thereby identify the antigens. An antibody against HER1, an antibody against HER2, an antibody against CD46, an antibody against ITGA3, an antibody against ICAM1, an antibody against ALCAM, an antibody against CD147, an antibody against C1qR, an antibody against CD44, an antibody against CD73, an antibody against EpCAM and an antibody against HGFR, each obtained by using the above methods.
La présente invention concerne un procédé au moyen duquel on peut classifier rapidement un grand nombre d'anticorps par rapport à des antigènes de surface cellulaire et qui constitue un procédé permettant d'identifier rapidement les antigènes desdits anticorps ainsi classifiés. L'invention porte également sur un procédé qui favorise l'utilisation des données utiles obtenues au moyen des procédés présentés ci-avant. On cherche également à utiliser un anticorps qui est efficace dans le traitement ou le diagnostic du cancer. Un procédé de classification des anticorps comprend les étapes suivantes: (1) la préparation d'une pluralité d'anticorps reconnaissant respectivement les antigènes de surface cellulaire; (2) la mise en contact de chacun de ces anticorps avec une cellule de la même espèce; (3) l'analyse, par cytométrie de flux, de chacune des cellules ayant été traitées dans l'étape (2) et l'obtention de données indiquant la réactivité de chaque anticorps à son antigène de surface cellulaire; et (4) la comparaison des données ainsi obtenues et la classification des anticorps individuels en fonction de la similarité. Un procédé d'identification d'antigènes comprend également les étapes suivantes: (5) la sélection d'au moins un anticorps dans le groupe d'anticorps formé dans l'étape (4) et l'identification des antigènes de ces derniers; et (6) à partir de l'hypothèse que les antigènes des anticorps appartenant à un groupe d'anticorps unique sont les mêmes ou sont fortement apparentés, l'établissement de relations entre les antigènes ayant été identifiés dans l'étape (5) et les groupes d'anticorps afin d'identifier les antigènes. Au moyen des procédés selon l'invention, on obtient les anticorps suivants: un anticorps dirigé contre HER1, un anticorps dirigé contre HER2, un anticorps dirigé contre CD46, un anticorps dirigé contre ITGA3, un anticorps dirigé contre ICAM1, un anticorps dirigé contre ALCAM, un anticorps dirigé contre CD147, un anticorps dirigé contre C1qR, un anticorps dirigé contre CD44, un anticorps dirigé contre CD73, un anticorps dirigé contre EpCAM et un anticorps dirigé contre HGFR.</description><language>eng ; fre ; jpn</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2008</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20080117&DB=EPODOC&CC=WO&NR=2008007648A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20080117&DB=EPODOC&CC=WO&NR=2008007648A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TSUTSUMI, SUSUMU</creatorcontrib><creatorcontrib>MATSUDA, KAZUKI</creatorcontrib><creatorcontrib>UKAI, YOSHINORI</creatorcontrib><creatorcontrib>AKAHORI, YASUSHI</creatorcontrib><creatorcontrib>HAYASHI, NOBUHIRO</creatorcontrib><creatorcontrib>TANAKA, MIHO</creatorcontrib><creatorcontrib>KUROSAWA, YOSHIKAZU</creatorcontrib><creatorcontrib>SUMITOMO, MARIKO</creatorcontrib><creatorcontrib>TAKASAKI, AKIHIKO</creatorcontrib><creatorcontrib>SUGIMOTO, MOTOTAKA</creatorcontrib><creatorcontrib>MORITA, MIWA</creatorcontrib><creatorcontrib>AZUMA, MASACHIKA</creatorcontrib><creatorcontrib>SUZUKI, KAZUHIRO</creatorcontrib><creatorcontrib>KUROSAWA, GENE</creatorcontrib><creatorcontrib>SATOU, NORIKO</creatorcontrib><creatorcontrib>SHIRAISHI, MAMORU</creatorcontrib><creatorcontrib>EGUCHI, KEIKO</creatorcontrib><creatorcontrib>SUGIOKA, ATSUSHI</creatorcontrib><creatorcontrib>MURAMATSU, CHIHO</creatorcontrib><title>METHOD OF CLASSIFYING ANTIGEN, METHOD OF IDENTIFYING ANTIGEN, METHOD OF OBTAINING ANTIBODY OR ANTIBODY SET, METHOD OF CONSTRUCTING ANTIBODY PANEL AND ANTIBODY OR ANTIBODY SET AND USE OF THE SAME</title><description>It is intended to provide a method whereby a plural number of antibodies against cell surface antigens are quickly classified and to provide a method whereby antigens of the thus classified antibodies are quickly identified. Further, it is intended to provide a method of promoting the utilization of the useful data obtained by the above methods. Furthermore, it is intended to provide an antibody which is effective in treating or diagnosing cancer. Namely, a method of classifying antibodies which comprises: (1) the step of preparing a plural number of antibodies respectively recognizing cell surface antigens; (2) the step of bringing each of these antibodies into contact with a cell of the same species; (3) the step of analyzing each of the cells having been treated in the step (2) by flow cytometry and thus obtaining data indicating the reactivity of each antibody with its cell surface antigen; and (4) the step of comparing the thus obtained data and classifying the individual antibodies depending on the similarity. A method of identifying antigens which further comprises: (5) the step of selecting one to several antibodies from each antibody group formed in the step (4) and identifying antigens thereof; and (6) on the assumption that antigens of the antibodies belonging to a single antibody group are the same or highly related to one another, making relations between the antigens having been identified in the step (5) and the antibody groups to thereby identify the antigens. An antibody against HER1, an antibody against HER2, an antibody against CD46, an antibody against ITGA3, an antibody against ICAM1, an antibody against ALCAM, an antibody against CD147, an antibody against C1qR, an antibody against CD44, an antibody against CD73, an antibody against EpCAM and an antibody against HGFR, each obtained by using the above methods.
La présente invention concerne un procédé au moyen duquel on peut classifier rapidement un grand nombre d'anticorps par rapport à des antigènes de surface cellulaire et qui constitue un procédé permettant d'identifier rapidement les antigènes desdits anticorps ainsi classifiés. L'invention porte également sur un procédé qui favorise l'utilisation des données utiles obtenues au moyen des procédés présentés ci-avant. On cherche également à utiliser un anticorps qui est efficace dans le traitement ou le diagnostic du cancer. Un procédé de classification des anticorps comprend les étapes suivantes: (1) la préparation d'une pluralité d'anticorps reconnaissant respectivement les antigènes de surface cellulaire; (2) la mise en contact de chacun de ces anticorps avec une cellule de la même espèce; (3) l'analyse, par cytométrie de flux, de chacune des cellules ayant été traitées dans l'étape (2) et l'obtention de données indiquant la réactivité de chaque anticorps à son antigène de surface cellulaire; et (4) la comparaison des données ainsi obtenues et la classification des anticorps individuels en fonction de la similarité. Un procédé d'identification d'antigènes comprend également les étapes suivantes: (5) la sélection d'au moins un anticorps dans le groupe d'anticorps formé dans l'étape (4) et l'identification des antigènes de ces derniers; et (6) à partir de l'hypothèse que les antigènes des anticorps appartenant à un groupe d'anticorps unique sont les mêmes ou sont fortement apparentés, l'établissement de relations entre les antigènes ayant été identifiés dans l'étape (5) et les groupes d'anticorps afin d'identifier les antigènes. Au moyen des procédés selon l'invention, on obtient les anticorps suivants: un anticorps dirigé contre HER1, un anticorps dirigé contre HER2, un anticorps dirigé contre CD46, un anticorps dirigé contre ITGA3, un anticorps dirigé contre ICAM1, un anticorps dirigé contre ALCAM, un anticorps dirigé contre CD147, un anticorps dirigé contre C1qR, un anticorps dirigé contre CD44, un anticorps dirigé contre CD73, un anticorps dirigé contre EpCAM et un anticorps dirigé contre HGFR.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2008</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDjk6xri4e-i4O-m4OzjGBzs6Rbp6eeu4OgX4unu6qejgJD2dHEFCuKU9ncKcfT0g0k6-btEKvgHIdjBriHIqp39_YJDgkKdQ1A0BDj6ufoAuS44zQBLhga7gswI8XBVCHb0deVhYE1LzClO5YXS3AzKbq4hzh66qQX58anFBYnJqXmpJfHh_kYGBhYGBuZmJhaOhsbEqQIA7wdOVg</recordid><startdate>20080117</startdate><enddate>20080117</enddate><creator>TSUTSUMI, SUSUMU</creator><creator>MATSUDA, KAZUKI</creator><creator>UKAI, YOSHINORI</creator><creator>AKAHORI, YASUSHI</creator><creator>HAYASHI, NOBUHIRO</creator><creator>TANAKA, MIHO</creator><creator>KUROSAWA, YOSHIKAZU</creator><creator>SUMITOMO, MARIKO</creator><creator>TAKASAKI, AKIHIKO</creator><creator>SUGIMOTO, MOTOTAKA</creator><creator>MORITA, MIWA</creator><creator>AZUMA, MASACHIKA</creator><creator>SUZUKI, KAZUHIRO</creator><creator>KUROSAWA, GENE</creator><creator>SATOU, NORIKO</creator><creator>SHIRAISHI, MAMORU</creator><creator>EGUCHI, KEIKO</creator><creator>SUGIOKA, ATSUSHI</creator><creator>MURAMATSU, CHIHO</creator><scope>EVB</scope></search><sort><creationdate>20080117</creationdate><title>METHOD OF CLASSIFYING ANTIGEN, METHOD OF IDENTIFYING ANTIGEN, METHOD OF OBTAINING ANTIBODY OR ANTIBODY SET, METHOD OF CONSTRUCTING ANTIBODY PANEL AND ANTIBODY OR ANTIBODY SET AND USE OF THE SAME</title><author>TSUTSUMI, SUSUMU ; MATSUDA, KAZUKI ; UKAI, YOSHINORI ; AKAHORI, YASUSHI ; HAYASHI, NOBUHIRO ; TANAKA, MIHO ; KUROSAWA, YOSHIKAZU ; SUMITOMO, MARIKO ; TAKASAKI, AKIHIKO ; SUGIMOTO, MOTOTAKA ; MORITA, MIWA ; AZUMA, MASACHIKA ; SUZUKI, KAZUHIRO ; KUROSAWA, GENE ; SATOU, NORIKO ; SHIRAISHI, MAMORU ; EGUCHI, KEIKO ; SUGIOKA, ATSUSHI ; MURAMATSU, CHIHO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2008007648A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; jpn</language><creationdate>2008</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>TSUTSUMI, SUSUMU</creatorcontrib><creatorcontrib>MATSUDA, KAZUKI</creatorcontrib><creatorcontrib>UKAI, YOSHINORI</creatorcontrib><creatorcontrib>AKAHORI, YASUSHI</creatorcontrib><creatorcontrib>HAYASHI, NOBUHIRO</creatorcontrib><creatorcontrib>TANAKA, MIHO</creatorcontrib><creatorcontrib>KUROSAWA, YOSHIKAZU</creatorcontrib><creatorcontrib>SUMITOMO, MARIKO</creatorcontrib><creatorcontrib>TAKASAKI, AKIHIKO</creatorcontrib><creatorcontrib>SUGIMOTO, MOTOTAKA</creatorcontrib><creatorcontrib>MORITA, MIWA</creatorcontrib><creatorcontrib>AZUMA, MASACHIKA</creatorcontrib><creatorcontrib>SUZUKI, KAZUHIRO</creatorcontrib><creatorcontrib>KUROSAWA, GENE</creatorcontrib><creatorcontrib>SATOU, NORIKO</creatorcontrib><creatorcontrib>SHIRAISHI, MAMORU</creatorcontrib><creatorcontrib>EGUCHI, KEIKO</creatorcontrib><creatorcontrib>SUGIOKA, ATSUSHI</creatorcontrib><creatorcontrib>MURAMATSU, CHIHO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TSUTSUMI, SUSUMU</au><au>MATSUDA, KAZUKI</au><au>UKAI, YOSHINORI</au><au>AKAHORI, YASUSHI</au><au>HAYASHI, NOBUHIRO</au><au>TANAKA, MIHO</au><au>KUROSAWA, YOSHIKAZU</au><au>SUMITOMO, MARIKO</au><au>TAKASAKI, AKIHIKO</au><au>SUGIMOTO, MOTOTAKA</au><au>MORITA, MIWA</au><au>AZUMA, MASACHIKA</au><au>SUZUKI, KAZUHIRO</au><au>KUROSAWA, GENE</au><au>SATOU, NORIKO</au><au>SHIRAISHI, MAMORU</au><au>EGUCHI, KEIKO</au><au>SUGIOKA, ATSUSHI</au><au>MURAMATSU, CHIHO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD OF CLASSIFYING ANTIGEN, METHOD OF IDENTIFYING ANTIGEN, METHOD OF OBTAINING ANTIBODY OR ANTIBODY SET, METHOD OF CONSTRUCTING ANTIBODY PANEL AND ANTIBODY OR ANTIBODY SET AND USE OF THE SAME</title><date>2008-01-17</date><risdate>2008</risdate><abstract>It is intended to provide a method whereby a plural number of antibodies against cell surface antigens are quickly classified and to provide a method whereby antigens of the thus classified antibodies are quickly identified. Further, it is intended to provide a method of promoting the utilization of the useful data obtained by the above methods. Furthermore, it is intended to provide an antibody which is effective in treating or diagnosing cancer. Namely, a method of classifying antibodies which comprises: (1) the step of preparing a plural number of antibodies respectively recognizing cell surface antigens; (2) the step of bringing each of these antibodies into contact with a cell of the same species; (3) the step of analyzing each of the cells having been treated in the step (2) by flow cytometry and thus obtaining data indicating the reactivity of each antibody with its cell surface antigen; and (4) the step of comparing the thus obtained data and classifying the individual antibodies depending on the similarity. A method of identifying antigens which further comprises: (5) the step of selecting one to several antibodies from each antibody group formed in the step (4) and identifying antigens thereof; and (6) on the assumption that antigens of the antibodies belonging to a single antibody group are the same or highly related to one another, making relations between the antigens having been identified in the step (5) and the antibody groups to thereby identify the antigens. An antibody against HER1, an antibody against HER2, an antibody against CD46, an antibody against ITGA3, an antibody against ICAM1, an antibody against ALCAM, an antibody against CD147, an antibody against C1qR, an antibody against CD44, an antibody against CD73, an antibody against EpCAM and an antibody against HGFR, each obtained by using the above methods.
La présente invention concerne un procédé au moyen duquel on peut classifier rapidement un grand nombre d'anticorps par rapport à des antigènes de surface cellulaire et qui constitue un procédé permettant d'identifier rapidement les antigènes desdits anticorps ainsi classifiés. L'invention porte également sur un procédé qui favorise l'utilisation des données utiles obtenues au moyen des procédés présentés ci-avant. On cherche également à utiliser un anticorps qui est efficace dans le traitement ou le diagnostic du cancer. Un procédé de classification des anticorps comprend les étapes suivantes: (1) la préparation d'une pluralité d'anticorps reconnaissant respectivement les antigènes de surface cellulaire; (2) la mise en contact de chacun de ces anticorps avec une cellule de la même espèce; (3) l'analyse, par cytométrie de flux, de chacune des cellules ayant été traitées dans l'étape (2) et l'obtention de données indiquant la réactivité de chaque anticorps à son antigène de surface cellulaire; et (4) la comparaison des données ainsi obtenues et la classification des anticorps individuels en fonction de la similarité. Un procédé d'identification d'antigènes comprend également les étapes suivantes: (5) la sélection d'au moins un anticorps dans le groupe d'anticorps formé dans l'étape (4) et l'identification des antigènes de ces derniers; et (6) à partir de l'hypothèse que les antigènes des anticorps appartenant à un groupe d'anticorps unique sont les mêmes ou sont fortement apparentés, l'établissement de relations entre les antigènes ayant été identifiés dans l'étape (5) et les groupes d'anticorps afin d'identifier les antigènes. Au moyen des procédés selon l'invention, on obtient les anticorps suivants: un anticorps dirigé contre HER1, un anticorps dirigé contre HER2, un anticorps dirigé contre CD46, un anticorps dirigé contre ITGA3, un anticorps dirigé contre ICAM1, un anticorps dirigé contre ALCAM, un anticorps dirigé contre CD147, un anticorps dirigé contre C1qR, un anticorps dirigé contre CD44, un anticorps dirigé contre CD73, un anticorps dirigé contre EpCAM et un anticorps dirigé contre HGFR.</abstract><oa>free_for_read</oa></addata></record> |
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| language | eng ; fre ; jpn |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | METHOD OF CLASSIFYING ANTIGEN, METHOD OF IDENTIFYING ANTIGEN, METHOD OF OBTAINING ANTIBODY OR ANTIBODY SET, METHOD OF CONSTRUCTING ANTIBODY PANEL AND ANTIBODY OR ANTIBODY SET AND USE OF THE SAME |
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