A SCREENING METHOD FOR IDENTIFYING NEW DRUGS
21 ABSTRACT A screening method for identifying new drugs A screening method for identifying a candidate to drug wherein said method 5 comprises the following steps: a) obtaining an expression vector which comprises a gene sequence codifying a naturally occurring pathogenic non- discriminating tRNA s...
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| creator | RIBAS DE POUPLANA, LLUIS BORI SANZ, TERESA |
| description | 21 ABSTRACT A screening method for identifying new drugs A screening method for identifying a candidate to drug wherein said method 5 comprises the following steps: a) obtaining an expression vector which comprises a gene sequence codifying a naturally occurring pathogenic non- discriminating tRNA synthetase; b) transforming isolated mammalian cells with the expression vector; c) growing the recombinant cells resulting from (b) in a nutrient medium under conditions which allow the expression of the 10 pathogenic tRNA synthetase, resulting the expression of the pathogenic tRNA synthetase into cell death or a decrease in the rate of cell division; d) providing a substance to be tested; and e) analyzing the resulting cell growth, wherein if there is an increase in cell growth, then the substance selectively inhibits the activity of the pathogenic tRNA synthetase and does 15 not affect to its cellular ortholog, resulting that said substance is a candidate to drug.
L'invention concerne un procédé de criblage destiné à identifier un médicament potentiel, ledit procédé 5 comprenant les étapes suivantes consistant à : a) obtenir un vecteur d'expression qui comprend une séquence de gènes codant une ARNt synthétase non discriminante pathogène apparaissant de façon naturelle ; b) transformer des cellules de mammifère que l'on a isolées à l'aide du vecteur d'expression ; c) cultiver les cellules recombinantes résultant de (b) dans un milieu nutritif et dans des conditions qui permettent l'expression de l'ARNt synthétase pathogène 10, l'expression de l'ARNt synthétase pathogène ayant pout conséquence une mort cellulaire ou une baisse du rythme de la division cellulaire ; d) fournir une substance à tester ; et e) analyser la croissance cellulaire résultante. Selon l'invention, s'il y a augmentation de la croissance cellulaire, alors la substance inhibe sélectivement l'activité de l'ARNt synthétase pathogène et n'affecte pas 15 son orthologue cellulaire, ce qui implique que ladite substance est un médicament potentiel. |
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L'invention concerne un procédé de criblage destiné à identifier un médicament potentiel, ledit procédé 5 comprenant les étapes suivantes consistant à : a) obtenir un vecteur d'expression qui comprend une séquence de gènes codant une ARNt synthétase non discriminante pathogène apparaissant de façon naturelle ; b) transformer des cellules de mammifère que l'on a isolées à l'aide du vecteur d'expression ; c) cultiver les cellules recombinantes résultant de (b) dans un milieu nutritif et dans des conditions qui permettent l'expression de l'ARNt synthétase pathogène 10, l'expression de l'ARNt synthétase pathogène ayant pout conséquence une mort cellulaire ou une baisse du rythme de la division cellulaire ; d) fournir une substance à tester ; et e) analyser la croissance cellulaire résultante. Selon l'invention, s'il y a augmentation de la croissance cellulaire, alors la substance inhibe sélectivement l'activité de l'ARNt synthétase pathogène et n'affecte pas 15 son orthologue cellulaire, ce qui implique que ladite substance est un médicament potentiel.</description><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2008</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20080103&DB=EPODOC&CC=WO&NR=2008000785A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20080103&DB=EPODOC&CC=WO&NR=2008000785A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>RIBAS DE POUPLANA, LLUIS</creatorcontrib><creatorcontrib>BORI SANZ, TERESA</creatorcontrib><title>A SCREENING METHOD FOR IDENTIFYING NEW DRUGS</title><description>21 ABSTRACT A screening method for identifying new drugs A screening method for identifying a candidate to drug wherein said method 5 comprises the following steps: a) obtaining an expression vector which comprises a gene sequence codifying a naturally occurring pathogenic non- discriminating tRNA synthetase; b) transforming isolated mammalian cells with the expression vector; c) growing the recombinant cells resulting from (b) in a nutrient medium under conditions which allow the expression of the 10 pathogenic tRNA synthetase, resulting the expression of the pathogenic tRNA synthetase into cell death or a decrease in the rate of cell division; d) providing a substance to be tested; and e) analyzing the resulting cell growth, wherein if there is an increase in cell growth, then the substance selectively inhibits the activity of the pathogenic tRNA synthetase and does 15 not affect to its cellular ortholog, resulting that said substance is a candidate to drug.
L'invention concerne un procédé de criblage destiné à identifier un médicament potentiel, ledit procédé 5 comprenant les étapes suivantes consistant à : a) obtenir un vecteur d'expression qui comprend une séquence de gènes codant une ARNt synthétase non discriminante pathogène apparaissant de façon naturelle ; b) transformer des cellules de mammifère que l'on a isolées à l'aide du vecteur d'expression ; c) cultiver les cellules recombinantes résultant de (b) dans un milieu nutritif et dans des conditions qui permettent l'expression de l'ARNt synthétase pathogène 10, l'expression de l'ARNt synthétase pathogène ayant pout conséquence une mort cellulaire ou une baisse du rythme de la division cellulaire ; d) fournir une substance à tester ; et e) analyser la croissance cellulaire résultante. Selon l'invention, s'il y a augmentation de la croissance cellulaire, alors la substance inhibe sélectivement l'activité de l'ARNt synthétase pathogène et n'affecte pas 15 son orthologue cellulaire, ce qui implique que ladite substance est un médicament potentiel.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2008</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNBxVAh2DnJ19fP0c1fwdQ3x8HdRcPMPUvB0cfUL8XSLBAn7uYYruASFugfzMLCmJeYUp_JCaW4GZTfXEGcP3dSC_PjU4oLE5NS81JL4cH8jAwMLAwMDcwtTR0Nj4lQBABdEJe8</recordid><startdate>20080103</startdate><enddate>20080103</enddate><creator>RIBAS DE POUPLANA, LLUIS</creator><creator>BORI SANZ, TERESA</creator><scope>EVB</scope></search><sort><creationdate>20080103</creationdate><title>A SCREENING METHOD FOR IDENTIFYING NEW DRUGS</title><author>RIBAS DE POUPLANA, LLUIS ; BORI SANZ, TERESA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2008000785A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2008</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>RIBAS DE POUPLANA, LLUIS</creatorcontrib><creatorcontrib>BORI SANZ, TERESA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>RIBAS DE POUPLANA, LLUIS</au><au>BORI SANZ, TERESA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>A SCREENING METHOD FOR IDENTIFYING NEW DRUGS</title><date>2008-01-03</date><risdate>2008</risdate><abstract>21 ABSTRACT A screening method for identifying new drugs A screening method for identifying a candidate to drug wherein said method 5 comprises the following steps: a) obtaining an expression vector which comprises a gene sequence codifying a naturally occurring pathogenic non- discriminating tRNA synthetase; b) transforming isolated mammalian cells with the expression vector; c) growing the recombinant cells resulting from (b) in a nutrient medium under conditions which allow the expression of the 10 pathogenic tRNA synthetase, resulting the expression of the pathogenic tRNA synthetase into cell death or a decrease in the rate of cell division; d) providing a substance to be tested; and e) analyzing the resulting cell growth, wherein if there is an increase in cell growth, then the substance selectively inhibits the activity of the pathogenic tRNA synthetase and does 15 not affect to its cellular ortholog, resulting that said substance is a candidate to drug.
L'invention concerne un procédé de criblage destiné à identifier un médicament potentiel, ledit procédé 5 comprenant les étapes suivantes consistant à : a) obtenir un vecteur d'expression qui comprend une séquence de gènes codant une ARNt synthétase non discriminante pathogène apparaissant de façon naturelle ; b) transformer des cellules de mammifère que l'on a isolées à l'aide du vecteur d'expression ; c) cultiver les cellules recombinantes résultant de (b) dans un milieu nutritif et dans des conditions qui permettent l'expression de l'ARNt synthétase pathogène 10, l'expression de l'ARNt synthétase pathogène ayant pout conséquence une mort cellulaire ou une baisse du rythme de la division cellulaire ; d) fournir une substance à tester ; et e) analyser la croissance cellulaire résultante. Selon l'invention, s'il y a augmentation de la croissance cellulaire, alors la substance inhibe sélectivement l'activité de l'ARNt synthétase pathogène et n'affecte pas 15 son orthologue cellulaire, ce qui implique que ladite substance est un médicament potentiel.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | A SCREENING METHOD FOR IDENTIFYING NEW DRUGS |
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