ISOTHERMAL METHODS FOR CREATING CLONAL SINGLE MOLECULE ARRAYS

The present invention is directed to a method for isothermal amplification of a plurality of different target nucleic acids, wherein the different target nucleic acids are amplified using universal primers and colonies produced thereby can be distinguished from each other. The method, therefore, gen...

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Hauptverfasser: SCHROTH, GARY PAUL, LLOYD, DAVID, ZHANG, LU
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creator SCHROTH, GARY PAUL
LLOYD, DAVID
ZHANG, LU
description The present invention is directed to a method for isothermal amplification of a plurality of different target nucleic acids, wherein the different target nucleic acids are amplified using universal primers and colonies produced thereby can be distinguished from each other. The method, therefore, generates distinct colonies of amplified nucleic acid sequences that can be analyzed by various means to yield information particular to each distinct colony. La présente invention concerne un procédé permettant l'amplification isothermique d'une pluralité d'acides nucléiques cibles différents, ces acides nucléiques cibles différents étant amplifiés à l'aide d'amorces universelles. Les colonies ainsi produites peuvent être distinguées les unes des autres. Par conséquent, le procédé génère des colonies distinctes de séquences d'acides nucléiques amplifiées qui peuvent être analysées par divers moyens afin d'apporter des informations sur chaque colonie distincte.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title ISOTHERMAL METHODS FOR CREATING CLONAL SINGLE MOLECULE ARRAYS
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