CDNA LIBRARY PREPARATION

New biochemical protocols for high throughput processing of mRNA samples into cDNA libraries with adaptor sequences compatible with automated sequencing systems are provided. The provided methods produces cDNA libraries which do not have 3' bias 5 associated with current cDNA library production...

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Hauptverfasser: WILLOUGHBY, DAVID, AUDEN, HUTCHISON, STEPHEN, KYLE, SIMONS, JAN, FREDRIK
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HUTCHISON, STEPHEN, KYLE
SIMONS, JAN, FREDRIK
description New biochemical protocols for high throughput processing of mRNA samples into cDNA libraries with adaptor sequences compatible with automated sequencing systems are provided. The provided methods produces cDNA libraries which do not have 3' bias 5 associated with current cDNA library production methods. New methods for the production of DNA libraries from DNA are also provided. L'invention concerne de nouveaux protocoles biochimiques de transformation à haut rendement d'échantillons d'ARNm en bibliothèques d'ADNc avec des séquences adaptatrices compatibles avec des systèmes de séquençage automatisés. Les méthodes de l'invention produisent des bibliothèques d'ADNc dont la distorsion de 3' à 5' n'est pas associée à des méthodes courantes de production de bibliothèques d'ADNc. On décrit de nouvelles méthodes de production de bibliothèques d'ADN à partir de l'ADN.
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The provided methods produces cDNA libraries which do not have 3' bias 5 associated with current cDNA library production methods. New methods for the production of DNA libraries from DNA are also provided. L'invention concerne de nouveaux protocoles biochimiques de transformation à haut rendement d'échantillons d'ARNm en bibliothèques d'ADNc avec des séquences adaptatrices compatibles avec des systèmes de séquençage automatisés. Les méthodes de l'invention produisent des bibliothèques d'ADNc dont la distorsion de 3' à 5' n'est pas associée à des méthodes courantes de production de bibliothèques d'ADNc. 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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title CDNA LIBRARY PREPARATION
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