GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID
The invention provides f lagellin-based fusion proteins (FBFP) useful for a variety of purposes, in bioremediation to remove metal ions from a liquid, to express an enzyme or an immunogen, nucleic acids encoding the FBFP, vectors containing the nucleic acids, and host cells harboring the vectors. Fu...
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| creator | LOUW, MAUREEN, ELIZABETH CRAMPTON, MICHAEL, CRAIG BERGER, ELDIE |
| description | The invention provides f lagellin-based fusion proteins (FBFP) useful for a variety of purposes, in bioremediation to remove metal ions from a liquid, to express an enzyme or an immunogen, nucleic acids encoding the FBFP, vectors containing the nucleic acids, and host cells harboring the vectors. Furthermore, the invention provides methods for obtaining over-expression and surface display of heterologous polypeptides in Gram-positive bacterial cells in Bacillus halodurans in particular. In addition, the invention features gene-disrupted bacteria useful for expressing the recombinant FBFP on their surfaces. Also included in the invention are genetic constructs useful for making FBFP and methods of using the FBFP.
L'invention concerne des protéines hybrides à base de flagelline (FBFP) utiles à divers égards, des acides nucléiques codant les FBFP, des vecteurs renfermant les acides nucléiques et des cellules hôtes abritant les vecteurs. L'invention concerne en outre des méthodes permettant d'obtenir une surexpression et un affichage en surface de polypeptides hétérologues dans des cellules bactériennes gram-positives. L'invention concerne en outre des bactéries à gène interrompu convenant pour l'expression de FBFP de recombinaison sur leurs surfaces. De plus, l'invention porte sur des constructions génétiques convenant pour la fabrication de FBFP et sur des méthodes d'utilisation des FBFP. |
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L'invention concerne des protéines hybrides à base de flagelline (FBFP) utiles à divers égards, des acides nucléiques codant les FBFP, des vecteurs renfermant les acides nucléiques et des cellules hôtes abritant les vecteurs. L'invention concerne en outre des méthodes permettant d'obtenir une surexpression et un affichage en surface de polypeptides hétérologues dans des cellules bactériennes gram-positives. L'invention concerne en outre des bactéries à gène interrompu convenant pour l'expression de FBFP de recombinaison sur leurs surfaces. De plus, l'invention porte sur des constructions génétiques convenant pour la fabrication de FBFP et sur des méthodes d'utilisation des FBFP.</description><language>eng ; fre</language><subject>CHEMISTRY ; METALLURGY ; ORGANIC CHEMISTRY ; PEPTIDES ; TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE</subject><creationdate>2007</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20070301&DB=EPODOC&CC=WO&NR=2006072845A3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20070301&DB=EPODOC&CC=WO&NR=2006072845A3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LOUW, MAUREEN, ELIZABETH</creatorcontrib><creatorcontrib>CRAMPTON, MICHAEL, CRAIG</creatorcontrib><creatorcontrib>BERGER, ELDIE</creatorcontrib><title>GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID</title><description>The invention provides f lagellin-based fusion proteins (FBFP) useful for a variety of purposes, in bioremediation to remove metal ions from a liquid, to express an enzyme or an immunogen, nucleic acids encoding the FBFP, vectors containing the nucleic acids, and host cells harboring the vectors. Furthermore, the invention provides methods for obtaining over-expression and surface display of heterologous polypeptides in Gram-positive bacterial cells in Bacillus halodurans in particular. In addition, the invention features gene-disrupted bacteria useful for expressing the recombinant FBFP on their surfaces. Also included in the invention are genetic constructs useful for making FBFP and methods of using the FBFP.
L'invention concerne des protéines hybrides à base de flagelline (FBFP) utiles à divers égards, des acides nucléiques codant les FBFP, des vecteurs renfermant les acides nucléiques et des cellules hôtes abritant les vecteurs. L'invention concerne en outre des méthodes permettant d'obtenir une surexpression et un affichage en surface de polypeptides hétérologues dans des cellules bactériennes gram-positives. L'invention concerne en outre des bactéries à gène interrompu convenant pour l'expression de FBFP de recombinaison sur leurs surfaces. De plus, l'invention porte sur des constructions génétiques convenant pour la fabrication de FBFP et sur des méthodes d'utilisation des FBFP.</description><subject>CHEMISTRY</subject><subject>METALLURGY</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2007</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNTbsKwkAQTGMh6j8s2BoI8dlekk1cuEe8vcQyBDkr0UD8E3_YEwRbq5lhXtPoVVmhoDZMjlqETOQOLQkJOUrJkBtVW2LSFQgoiG1TOyyglKIKPmmoUOPqp-NM8MdvmIyG2hqHpBmELqBhhFCwqEwb9k0JCl0gIchQWqPCg6RTQ8U8mlz72-gXX5xFyxJdfoz98Oj8OPQXf_fP7mzSJNkl-_Sw2Yr1-r_UG8w7Qbw</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>LOUW, MAUREEN, ELIZABETH</creator><creator>CRAMPTON, MICHAEL, CRAIG</creator><creator>BERGER, ELDIE</creator><scope>EVB</scope></search><sort><creationdate>20070301</creationdate><title>GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID</title><author>LOUW, MAUREEN, ELIZABETH ; CRAMPTON, MICHAEL, CRAIG ; BERGER, ELDIE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2006072845A33</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2007</creationdate><topic>CHEMISTRY</topic><topic>METALLURGY</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE</topic><toplevel>online_resources</toplevel><creatorcontrib>LOUW, MAUREEN, ELIZABETH</creatorcontrib><creatorcontrib>CRAMPTON, MICHAEL, CRAIG</creatorcontrib><creatorcontrib>BERGER, ELDIE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LOUW, MAUREEN, ELIZABETH</au><au>CRAMPTON, MICHAEL, CRAIG</au><au>BERGER, ELDIE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID</title><date>2007-03-01</date><risdate>2007</risdate><abstract>The invention provides f lagellin-based fusion proteins (FBFP) useful for a variety of purposes, in bioremediation to remove metal ions from a liquid, to express an enzyme or an immunogen, nucleic acids encoding the FBFP, vectors containing the nucleic acids, and host cells harboring the vectors. Furthermore, the invention provides methods for obtaining over-expression and surface display of heterologous polypeptides in Gram-positive bacterial cells in Bacillus halodurans in particular. In addition, the invention features gene-disrupted bacteria useful for expressing the recombinant FBFP on their surfaces. Also included in the invention are genetic constructs useful for making FBFP and methods of using the FBFP.
L'invention concerne des protéines hybrides à base de flagelline (FBFP) utiles à divers égards, des acides nucléiques codant les FBFP, des vecteurs renfermant les acides nucléiques et des cellules hôtes abritant les vecteurs. L'invention concerne en outre des méthodes permettant d'obtenir une surexpression et un affichage en surface de polypeptides hétérologues dans des cellules bactériennes gram-positives. L'invention concerne en outre des bactéries à gène interrompu convenant pour l'expression de FBFP de recombinaison sur leurs surfaces. De plus, l'invention porte sur des constructions génétiques convenant pour la fabrication de FBFP et sur des méthodes d'utilisation des FBFP.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | CHEMISTRY METALLURGY ORGANIC CHEMISTRY PEPTIDES TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE |
| title | GRAM POSITIVE BACTERIAL CELLS COMPRISING A DISRUPTED FLAGELLIN GENE, FLAGELLIN-BASED FUSION PROTEINS AND USE IN REMOVAL OF METAL IONS FROM A LIQUID |
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