DIRECT NUCLEIC ACID DETECTION IN BODILY FLUIDS

The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide prim...

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Hauptverfasser: LYAMICHEV, VICTOR, ALLAWI, HATIM, T, JARVIS, NANCY, ROEVEN, ROBERT, HALL, JEFF, G, LUKOWIAK, ANDREW, A
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creator LYAMICHEV, VICTOR
ALLAWI, HATIM, T
JARVIS, NANCY
ROEVEN, ROBERT
HALL, JEFF, G
LUKOWIAK, ANDREW, A
description The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays. L'invention concerne des méthodes et des routines médicales permettant de développer et d'optimiser des essais de détection d'acide nucléique destinés à être utilisés dans la recherche fondamentale, la recherche clinique, et pour le développement d'essais de détection clinique. L'invention porte notamment sur des méthodes de conception d'amorces oligonucléotidiques destinées être utilisées dans des réactions d'amplification multiplex. L'invention se rapporte aussi à des méthodes d'optimisation de réactions d'amplification multiplex, ainsi qu'à des méthodes d'essais de génération de signaux et de cibles combinés.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title DIRECT NUCLEIC ACID DETECTION IN BODILY FLUIDS
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