METHOD FOR DETECTION OF ALKYLATED CYTOSINE IN DNA
There is described a method for detecting alkylated cytosine in double stranded DNA employing one or more enzymes that differentially modify alkylated cytosine and cytosine. At least one region of the DNA is converted to single stranded DNA and the enzyme is reacted with a target region in the singl...
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| creator | TODD, ALISON VELYIAN APPLEGATE, TANYA LYNN FUERY, CAROLINE JANE |
| description | There is described a method for detecting alkylated cytosine in double stranded DNA employing one or more enzymes that differentially modify alkylated cytosine and cytosine. At least one region of the DNA is converted to single stranded DNA and the enzyme is reacted with a target region in the single stranded DNA. The presence or level of alkylated cytosine in the target region is detected by determining the level of enzymatic modification of the target region by the enzyme.
Cette invention se rapporte à un procédé servant à détecter la cytosine alkylée dans un ADN bicaténaire, en utilisant une ou plusieurs enzymes qui modifient de façon différentielle la cytosine alkylée et la cytosine. Au moins une zone de l'ADN est convertie en ADN monocaténaire et l'enzyme est amenée à réagir avec une zone cible de l'ADN monocaténaire. On détermine la présence ou le niveau de cytosine alkylée dans la zone cible en déterminant le niveau de modification enzymatique de la zone cible par l'enzyme. |
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Cette invention se rapporte à un procédé servant à détecter la cytosine alkylée dans un ADN bicaténaire, en utilisant une ou plusieurs enzymes qui modifient de façon différentielle la cytosine alkylée et la cytosine. Au moins une zone de l'ADN est convertie en ADN monocaténaire et l'enzyme est amenée à réagir avec une zone cible de l'ADN monocaténaire. On détermine la présence ou le niveau de cytosine alkylée dans la zone cible en déterminant le niveau de modification enzymatique de la zone cible par l'enzyme.</description><edition>7</edition><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2005</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20050113&DB=EPODOC&CC=WO&NR=2005003381A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20050113&DB=EPODOC&CC=WO&NR=2005003381A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TODD, ALISON VELYIAN</creatorcontrib><creatorcontrib>APPLEGATE, TANYA LYNN</creatorcontrib><creatorcontrib>FUERY, CAROLINE JANE</creatorcontrib><title>METHOD FOR DETECTION OF ALKYLATED CYTOSINE IN DNA</title><description>There is described a method for detecting alkylated cytosine in double stranded DNA employing one or more enzymes that differentially modify alkylated cytosine and cytosine. At least one region of the DNA is converted to single stranded DNA and the enzyme is reacted with a target region in the single stranded DNA. The presence or level of alkylated cytosine in the target region is detected by determining the level of enzymatic modification of the target region by the enzyme.
Cette invention se rapporte à un procédé servant à détecter la cytosine alkylée dans un ADN bicaténaire, en utilisant une ou plusieurs enzymes qui modifient de façon différentielle la cytosine alkylée et la cytosine. Au moins une zone de l'ADN est convertie en ADN monocaténaire et l'enzyme est amenée à réagir avec une zone cible de l'ADN monocaténaire. On détermine la présence ou le niveau de cytosine alkylée dans la zone cible en déterminant le niveau de modification enzymatique de la zone cible par l'enzyme.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2005</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDD0dQ3x8HdRcPMPUnBxDXF1DvH091Pwd1Nw9PGO9HEMcXVRcI4M8Q_29HNV8PRTcPFz5GFgTUvMKU7lhdLcDMpuriHOHrqpBfnxqcUFicmpeakl8eH-RgYGpgYGxsYWho6GxsSpAgDPDScj</recordid><startdate>20050113</startdate><enddate>20050113</enddate><creator>TODD, ALISON VELYIAN</creator><creator>APPLEGATE, TANYA LYNN</creator><creator>FUERY, CAROLINE JANE</creator><scope>EVB</scope></search><sort><creationdate>20050113</creationdate><title>METHOD FOR DETECTION OF ALKYLATED CYTOSINE IN DNA</title><author>TODD, ALISON VELYIAN ; APPLEGATE, TANYA LYNN ; FUERY, CAROLINE JANE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2005003381A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2005</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>TODD, ALISON VELYIAN</creatorcontrib><creatorcontrib>APPLEGATE, TANYA LYNN</creatorcontrib><creatorcontrib>FUERY, CAROLINE JANE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TODD, ALISON VELYIAN</au><au>APPLEGATE, TANYA LYNN</au><au>FUERY, CAROLINE JANE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD FOR DETECTION OF ALKYLATED CYTOSINE IN DNA</title><date>2005-01-13</date><risdate>2005</risdate><abstract>There is described a method for detecting alkylated cytosine in double stranded DNA employing one or more enzymes that differentially modify alkylated cytosine and cytosine. At least one region of the DNA is converted to single stranded DNA and the enzyme is reacted with a target region in the single stranded DNA. The presence or level of alkylated cytosine in the target region is detected by determining the level of enzymatic modification of the target region by the enzyme.
Cette invention se rapporte à un procédé servant à détecter la cytosine alkylée dans un ADN bicaténaire, en utilisant une ou plusieurs enzymes qui modifient de façon différentielle la cytosine alkylée et la cytosine. Au moins une zone de l'ADN est convertie en ADN monocaténaire et l'enzyme est amenée à réagir avec une zone cible de l'ADN monocaténaire. On détermine la présence ou le niveau de cytosine alkylée dans la zone cible en déterminant le niveau de modification enzymatique de la zone cible par l'enzyme.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHOD FOR DETECTION OF ALKYLATED CYTOSINE IN DNA |
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