DETECTION OF PROTEASE-RESISTANT PRION PROTEIN FOLLOWING A SPONTANEOUS TRANSFORMATION REACTION
Die vorliegende Erfindung betrifft Verfahren zum Nachweis von infektiösem bzw. pathogenem Prion-Protein mit verbesserter Sensitivität. Hierzu erfolgt eine de novo Bildung von Protease-resistentem Prion-Protein PrPSc durch spontane Transformationsreaktion mittels Wechselwirkung von nichtpathogenem Pr...
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| creator | GASSNER, DIETER GOLLA, RUTH |
| description | Die vorliegende Erfindung betrifft Verfahren zum Nachweis von infektiösem bzw. pathogenem Prion-Protein mit verbesserter Sensitivität. Hierzu erfolgt eine de novo Bildung von Protease-resistentem Prion-Protein PrPSc durch spontane Transformationsreaktion mittels Wechselwirkung von nichtpathogenem Prion-Protein PrPc in der Probe mit Protease-sensitiven niedermolekularen PrPSc-Aggregaten, um höhermolekulare Protease-resistente PrPSc-Aggregate zu bilden.
The invention relates to methods for detecting infectious or pathogenic prion protein with improved sensitivity. According to the invention, a protease-resistant prion protein PrPSc is freshly formed by a spontaneous transformation reaction by means of interaction of non-pathogenic prion protein PrPc in the sample with low-molecular protease-sensitive PrPSc aggregates, in order to form high-molecular protease-resistant PrPSc aggregates.
L'invention concerne un procédé de détection, à sensibilité accrue, de prion-protéine infectieuse ou pathogène. A cet effet, on effectue une formation de novo une prion-protéine résistant à la protéase PrPSc, par réaction de transformation spontanée par interaction de prion-protéine non-pathogène PrPc dans l'échantillon, avec des agrégats de PrPSc faiblement moléculaires, sensibles à la protéase, en vue de former des agrégats de PrPSc hautement moléculaires, résistant à la protéase. |
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The invention relates to methods for detecting infectious or pathogenic prion protein with improved sensitivity. According to the invention, a protease-resistant prion protein PrPSc is freshly formed by a spontaneous transformation reaction by means of interaction of non-pathogenic prion protein PrPc in the sample with low-molecular protease-sensitive PrPSc aggregates, in order to form high-molecular protease-resistant PrPSc aggregates.
L'invention concerne un procédé de détection, à sensibilité accrue, de prion-protéine infectieuse ou pathogène. A cet effet, on effectue une formation de novo une prion-protéine résistant à la protéase PrPSc, par réaction de transformation spontanée par interaction de prion-protéine non-pathogène PrPc dans l'échantillon, avec des agrégats de PrPSc faiblement moléculaires, sensibles à la protéase, en vue de former des agrégats de PrPSc hautement moléculaires, résistant à la protéase.</description><edition>7</edition><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2005</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20050106&DB=EPODOC&CC=WO&NR=2005001481A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20050106&DB=EPODOC&CC=WO&NR=2005001481A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>GASSNER, DIETER</creatorcontrib><creatorcontrib>GOLLA, RUTH</creatorcontrib><title>DETECTION OF PROTEASE-RESISTANT PRION PROTEIN FOLLOWING A SPONTANEOUS TRANSFORMATION REACTION</title><description>Die vorliegende Erfindung betrifft Verfahren zum Nachweis von infektiösem bzw. pathogenem Prion-Protein mit verbesserter Sensitivität. Hierzu erfolgt eine de novo Bildung von Protease-resistentem Prion-Protein PrPSc durch spontane Transformationsreaktion mittels Wechselwirkung von nichtpathogenem Prion-Protein PrPc in der Probe mit Protease-sensitiven niedermolekularen PrPSc-Aggregaten, um höhermolekulare Protease-resistente PrPSc-Aggregate zu bilden.
The invention relates to methods for detecting infectious or pathogenic prion protein with improved sensitivity. According to the invention, a protease-resistant prion protein PrPSc is freshly formed by a spontaneous transformation reaction by means of interaction of non-pathogenic prion protein PrPc in the sample with low-molecular protease-sensitive PrPSc aggregates, in order to form high-molecular protease-resistant PrPSc aggregates.
L'invention concerne un procédé de détection, à sensibilité accrue, de prion-protéine infectieuse ou pathogène. A cet effet, on effectue une formation de novo une prion-protéine résistant à la protéase PrPSc, par réaction de transformation spontanée par interaction de prion-protéine non-pathogène PrPc dans l'échantillon, avec des agrégats de PrPSc faiblement moléculaires, sensibles à la protéase, en vue de former des agrégats de PrPSc hautement moléculaires, résistant à la protéase.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2005</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjMsKwjAQRbNxIeo_DLguJD7A7VAnGqiZkkzpSkqRuBIt1P_HGvwAVxfuOZy5uh5JqBTHHthCHVgIIxWBoouCXqbryzJwHixXFbfOnwAh1uwnhbiJIAF9tBwumFOBMDeXanbvH2Na_Xah1pakPBdpeHVpHPpbeqZ31_JG673WZncwaLb_WR8dIjRF</recordid><startdate>20050106</startdate><enddate>20050106</enddate><creator>GASSNER, DIETER</creator><creator>GOLLA, RUTH</creator><scope>EVB</scope></search><sort><creationdate>20050106</creationdate><title>DETECTION OF PROTEASE-RESISTANT PRION PROTEIN FOLLOWING A SPONTANEOUS TRANSFORMATION REACTION</title><author>GASSNER, DIETER ; GOLLA, RUTH</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2005001481A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2005</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>GASSNER, DIETER</creatorcontrib><creatorcontrib>GOLLA, RUTH</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>GASSNER, DIETER</au><au>GOLLA, RUTH</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>DETECTION OF PROTEASE-RESISTANT PRION PROTEIN FOLLOWING A SPONTANEOUS TRANSFORMATION REACTION</title><date>2005-01-06</date><risdate>2005</risdate><abstract>Die vorliegende Erfindung betrifft Verfahren zum Nachweis von infektiösem bzw. pathogenem Prion-Protein mit verbesserter Sensitivität. Hierzu erfolgt eine de novo Bildung von Protease-resistentem Prion-Protein PrPSc durch spontane Transformationsreaktion mittels Wechselwirkung von nichtpathogenem Prion-Protein PrPc in der Probe mit Protease-sensitiven niedermolekularen PrPSc-Aggregaten, um höhermolekulare Protease-resistente PrPSc-Aggregate zu bilden.
The invention relates to methods for detecting infectious or pathogenic prion protein with improved sensitivity. According to the invention, a protease-resistant prion protein PrPSc is freshly formed by a spontaneous transformation reaction by means of interaction of non-pathogenic prion protein PrPc in the sample with low-molecular protease-sensitive PrPSc aggregates, in order to form high-molecular protease-resistant PrPSc aggregates.
L'invention concerne un procédé de détection, à sensibilité accrue, de prion-protéine infectieuse ou pathogène. A cet effet, on effectue une formation de novo une prion-protéine résistant à la protéase PrPSc, par réaction de transformation spontanée par interaction de prion-protéine non-pathogène PrPc dans l'échantillon, avec des agrégats de PrPSc faiblement moléculaires, sensibles à la protéase, en vue de former des agrégats de PrPSc hautement moléculaires, résistant à la protéase.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE |
| title | DETECTION OF PROTEASE-RESISTANT PRION PROTEIN FOLLOWING A SPONTANEOUS TRANSFORMATION REACTION |
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