METHOD FOR DETECTING TOXIC AND NON-TOXIC CYANOBACTERIA

This invention is related to a method for detecting toxic and non-toxic cyanobacteria. The method comprises that nucleic acid from a biological sample is brought into contact with an oligonucleotide designed to be specific for the mcy gene, in particular mcyE and/or mcyD, and with an oligonucleotide...

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Hauptverfasser: VENTURA, STEFANO, CASTIGLIONI, BIANCA, SIVONEN, KAARINA, RAJANIEMI, PIRJO, ROUHIAINEN, LEO, WILMOTTE, ANNICK, BALTHASART, PIERRE, MUGNAI, MARIA ANGELA, DE BELLIS, GIANLUCA, FEWER, DAVID, RANTALA, ANNE, FROSINI, ANDREA, BOUTTE, CHRISTOPHE, RIZZI, ERMANNO, GRUBISIC, STANA
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creator VENTURA, STEFANO
CASTIGLIONI, BIANCA
SIVONEN, KAARINA
RAJANIEMI, PIRJO
ROUHIAINEN, LEO
WILMOTTE, ANNICK
BALTHASART, PIERRE
MUGNAI, MARIA ANGELA
DE BELLIS, GIANLUCA
FEWER, DAVID
RANTALA, ANNE
FROSINI, ANDREA
BOUTTE, CHRISTOPHE
RIZZI, ERMANNO
GRUBISIC, STANA
description This invention is related to a method for detecting toxic and non-toxic cyanobacteria. The method comprises that nucleic acid from a biological sample is brought into contact with an oligonucleotide designed to be specific for the mcy gene, in particular mcyE and/or mcyD, and with an oligonucleotide designed to be specific for 16SrDNA, and the presence or absence of toxic cyanobacteria is detected by a suitable molecular biology method. The invention is related also to oligonucleotides used in the method. L'invention se rapporte à un procédé de détection de cyanobatéries toxiques et non toxiques. Ledit procédé consiste à mettre en contact un acide nucléique provenant d'un échantillon biologique avec un oligonucléotide conçu pour être spécifique du gène mcy, notamment de mcyE et/ou mcyD, et avec un oligonucléotide conçu pour être spécifique de l'ADNr 16S, puis à détecter la présence ou l'absence de cyanobactéries toxiques au moyen d'un procédé de biologie moléculaire adapté. L'invention se rapporte également aux oligonucléotides utilisés dans ce procédé.
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The method comprises that nucleic acid from a biological sample is brought into contact with an oligonucleotide designed to be specific for the mcy gene, in particular mcyE and/or mcyD, and with an oligonucleotide designed to be specific for 16SrDNA, and the presence or absence of toxic cyanobacteria is detected by a suitable molecular biology method. The invention is related also to oligonucleotides used in the method. L'invention se rapporte à un procédé de détection de cyanobatéries toxiques et non toxiques. Ledit procédé consiste à mettre en contact un acide nucléique provenant d'un échantillon biologique avec un oligonucléotide conçu pour être spécifique du gène mcy, notamment de mcyE et/ou mcyD, et avec un oligonucléotide conçu pour être spécifique de l'ADNr 16S, puis à détecter la présence ou l'absence de cyanobactéries toxiques au moyen d'un procédé de biologie moléculaire adapté. 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The method comprises that nucleic acid from a biological sample is brought into contact with an oligonucleotide designed to be specific for the mcy gene, in particular mcyE and/or mcyD, and with an oligonucleotide designed to be specific for 16SrDNA, and the presence or absence of toxic cyanobacteria is detected by a suitable molecular biology method. The invention is related also to oligonucleotides used in the method. L'invention se rapporte à un procédé de détection de cyanobatéries toxiques et non toxiques. Ledit procédé consiste à mettre en contact un acide nucléique provenant d'un échantillon biologique avec un oligonucléotide conçu pour être spécifique du gène mcy, notamment de mcyE et/ou mcyD, et avec un oligonucléotide conçu pour être spécifique de l'ADNr 16S, puis à détecter la présence ou l'absence de cyanobactéries toxiques au moyen d'un procédé de biologie moléculaire adapté. L'invention se rapporte également aux oligonucléotides utilisés dans ce procédé.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title METHOD FOR DETECTING TOXIC AND NON-TOXIC CYANOBACTERIA
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