METHOD FOR DETECTING TOXIC AND NON-TOXIC CYANOBACTERIA
This invention is related to a method for detecting toxic and non-toxic cyanobacteria. The method comprises that nucleic acid from a biological sample is brought into contact with an oligonucleotide designed to be specific for the mcy gene, in particular mcyE and/or mcyD, and with an oligonucleotide...
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creator | VENTURA, STEFANO CASTIGLIONI, BIANCA SIVONEN, KAARINA RAJANIEMI, PIRJO ROUHIAINEN, LEO WILMOTTE, ANNICK BALTHASART, PIERRE MUGNAI, MARIA ANGELA DE BELLIS, GIANLUCA FEWER, DAVID RANTALA, ANNE FROSINI, ANDREA BOUTTE, CHRISTOPHE RIZZI, ERMANNO GRUBISIC, STANA |
description | This invention is related to a method for detecting toxic and non-toxic cyanobacteria. The method comprises that nucleic acid from a biological sample is brought into contact with an oligonucleotide designed to be specific for the mcy gene, in particular mcyE and/or mcyD, and with an oligonucleotide designed to be specific for 16SrDNA, and the presence or absence of toxic cyanobacteria is detected by a suitable molecular biology method. The invention is related also to oligonucleotides used in the method.
L'invention se rapporte à un procédé de détection de cyanobatéries toxiques et non toxiques. Ledit procédé consiste à mettre en contact un acide nucléique provenant d'un échantillon biologique avec un oligonucléotide conçu pour être spécifique du gène mcy, notamment de mcyE et/ou mcyD, et avec un oligonucléotide conçu pour être spécifique de l'ADNr 16S, puis à détecter la présence ou l'absence de cyanobactéries toxiques au moyen d'un procédé de biologie moléculaire adapté. L'invention se rapporte également aux oligonucléotides utilisés dans ce procédé. |
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L'invention se rapporte à un procédé de détection de cyanobatéries toxiques et non toxiques. Ledit procédé consiste à mettre en contact un acide nucléique provenant d'un échantillon biologique avec un oligonucléotide conçu pour être spécifique du gène mcy, notamment de mcyE et/ou mcyD, et avec un oligonucléotide conçu pour être spécifique de l'ADNr 16S, puis à détecter la présence ou l'absence de cyanobactéries toxiques au moyen d'un procédé de biologie moléculaire adapté. L'invention se rapporte également aux oligonucléotides utilisés dans ce procédé.</description><edition>7</edition><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2004</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20041202&DB=EPODOC&CC=WO&NR=2004104211A2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20041202&DB=EPODOC&CC=WO&NR=2004104211A2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>VENTURA, STEFANO</creatorcontrib><creatorcontrib>CASTIGLIONI, BIANCA</creatorcontrib><creatorcontrib>SIVONEN, KAARINA</creatorcontrib><creatorcontrib>RAJANIEMI, PIRJO</creatorcontrib><creatorcontrib>ROUHIAINEN, LEO</creatorcontrib><creatorcontrib>WILMOTTE, ANNICK</creatorcontrib><creatorcontrib>BALTHASART, PIERRE</creatorcontrib><creatorcontrib>MUGNAI, MARIA ANGELA</creatorcontrib><creatorcontrib>DE BELLIS, GIANLUCA</creatorcontrib><creatorcontrib>FEWER, DAVID</creatorcontrib><creatorcontrib>RANTALA, ANNE</creatorcontrib><creatorcontrib>FROSINI, ANDREA</creatorcontrib><creatorcontrib>BOUTTE, CHRISTOPHE</creatorcontrib><creatorcontrib>RIZZI, ERMANNO</creatorcontrib><creatorcontrib>GRUBISIC, STANA</creatorcontrib><title>METHOD FOR DETECTING TOXIC AND NON-TOXIC CYANOBACTERIA</title><description>This invention is related to a method for detecting toxic and non-toxic cyanobacteria. The method comprises that nucleic acid from a biological sample is brought into contact with an oligonucleotide designed to be specific for the mcy gene, in particular mcyE and/or mcyD, and with an oligonucleotide designed to be specific for 16SrDNA, and the presence or absence of toxic cyanobacteria is detected by a suitable molecular biology method. The invention is related also to oligonucleotides used in the method.
L'invention se rapporte à un procédé de détection de cyanobatéries toxiques et non toxiques. Ledit procédé consiste à mettre en contact un acide nucléique provenant d'un échantillon biologique avec un oligonucléotide conçu pour être spécifique du gène mcy, notamment de mcyE et/ou mcyD, et avec un oligonucléotide conçu pour être spécifique de l'ADNr 16S, puis à détecter la présence ou l'absence de cyanobactéries toxiques au moyen d'un procédé de biologie moléculaire adapté. 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The method comprises that nucleic acid from a biological sample is brought into contact with an oligonucleotide designed to be specific for the mcy gene, in particular mcyE and/or mcyD, and with an oligonucleotide designed to be specific for 16SrDNA, and the presence or absence of toxic cyanobacteria is detected by a suitable molecular biology method. The invention is related also to oligonucleotides used in the method.
L'invention se rapporte à un procédé de détection de cyanobatéries toxiques et non toxiques. Ledit procédé consiste à mettre en contact un acide nucléique provenant d'un échantillon biologique avec un oligonucléotide conçu pour être spécifique du gène mcy, notamment de mcyE et/ou mcyD, et avec un oligonucléotide conçu pour être spécifique de l'ADNr 16S, puis à détecter la présence ou l'absence de cyanobactéries toxiques au moyen d'un procédé de biologie moléculaire adapté. L'invention se rapporte également aux oligonucléotides utilisés dans ce procédé.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
title | METHOD FOR DETECTING TOXIC AND NON-TOXIC CYANOBACTERIA |
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