NOVEL CELL LINE, PREPARATION METHOD THEREOF AND USE OF SAME, PARTICULARLY FOR MONITORING CHANGES IN CHROMATIN TEXTURE
The invention relates to cells from a stable cell line, e. g. monoclonal, having at least one inactive X chromosome, expressing a fusion protein comprising a protein which is associated with heterochromatin, such as a histone variant, and a fluorescent protein. The invention also relates to a method...
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| creator | DESLEE, DIDIER, LOUIS, ARTHUR VOURC'H, CLAIRE, NADINE, MARIE PERCHE, PIERRE-YVES HELIOT, LAURENT |
| description | The invention relates to cells from a stable cell line, e. g. monoclonal, having at least one inactive X chromosome, expressing a fusion protein comprising a protein which is associated with heterochromatin, such as a histone variant, and a fluorescent protein. The invention also relates to a method of preparing the stable cell line and to the use of said stable cell line cells for the screening of agents that can modify the texture of the chromatin and, in particular, the inactive state of the inactive X chromosome.
L'invention concerne des cellules de lignée cellulaire stable, notamment monoclonale, possédant au moins un chromosome X inactif, exprimant une protéine de fusion comprenant une protéine associé à l'hétérochromatine, notamment un variant d'histone, et une protéine fluorescente. L'invention concerne également un procédé de préparation de ladite lignée cellulaire stable et l'utilisation desdites cellules de lignée cellulaire stable pour cribler des agents susceptibles de modifier la texture de la chromatine et notamment l'état d'inactivation du chromosome X inactif. |
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L'invention concerne des cellules de lignée cellulaire stable, notamment monoclonale, possédant au moins un chromosome X inactif, exprimant une protéine de fusion comprenant une protéine associé à l'hétérochromatine, notamment un variant d'histone, et une protéine fluorescente. L'invention concerne également un procédé de préparation de ladite lignée cellulaire stable et l'utilisation desdites cellules de lignée cellulaire stable pour cribler des agents susceptibles de modifier la texture de la chromatine et notamment l'état d'inactivation du chromosome X inactif.</description><edition>7</edition><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2004</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20040902&DB=EPODOC&CC=WO&NR=2004074466A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20040902&DB=EPODOC&CC=WO&NR=2004074466A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DESLEE, DIDIER, LOUIS, ARTHUR</creatorcontrib><creatorcontrib>VOURC'H, CLAIRE, NADINE, MARIE</creatorcontrib><creatorcontrib>PERCHE, PIERRE-YVES</creatorcontrib><creatorcontrib>HELIOT, LAURENT</creatorcontrib><title>NOVEL CELL LINE, PREPARATION METHOD THEREOF AND USE OF SAME, PARTICULARLY FOR MONITORING CHANGES IN CHROMATIN TEXTURE</title><description>The invention relates to cells from a stable cell line, e. g. monoclonal, having at least one inactive X chromosome, expressing a fusion protein comprising a protein which is associated with heterochromatin, such as a histone variant, and a fluorescent protein. The invention also relates to a method of preparing the stable cell line and to the use of said stable cell line cells for the screening of agents that can modify the texture of the chromatin and, in particular, the inactive state of the inactive X chromosome.
L'invention concerne des cellules de lignée cellulaire stable, notamment monoclonale, possédant au moins un chromosome X inactif, exprimant une protéine de fusion comprenant une protéine associé à l'hétérochromatine, notamment un variant d'histone, et une protéine fluorescente. L'invention concerne également un procédé de préparation de ladite lignée cellulaire stable et l'utilisation desdites cellules de lignée cellulaire stable pour cribler des agents susceptibles de modifier la texture de la chromatine et notamment l'état d'inactivation du chromosome X inactif.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2004</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjMsKwjAQAHvxIOo_LHhVqFrqOaTbJpDsyjb1cSpF4km0UP1_K_gBnmYOw0yTN_ERHWh0DpwlXMFB8KBEBcsEHoPhAoJBQS5BUQFNjTBqrfy3VRKsbpwSd4GSBTyTDSyWKtBGUYU1WBpV2I9HgoDn0AjOk8mtuw9x8eMsWZYYtFnH_tnGoe-u8RFf7Ym3aZql-yzLc7XZ_Vd9AKVROe8</recordid><startdate>20040902</startdate><enddate>20040902</enddate><creator>DESLEE, DIDIER, LOUIS, ARTHUR</creator><creator>VOURC'H, CLAIRE, NADINE, MARIE</creator><creator>PERCHE, PIERRE-YVES</creator><creator>HELIOT, LAURENT</creator><scope>EVB</scope></search><sort><creationdate>20040902</creationdate><title>NOVEL CELL LINE, PREPARATION METHOD THEREOF AND USE OF SAME, PARTICULARLY FOR MONITORING CHANGES IN CHROMATIN TEXTURE</title><author>DESLEE, DIDIER, LOUIS, ARTHUR ; VOURC'H, CLAIRE, NADINE, MARIE ; PERCHE, PIERRE-YVES ; HELIOT, LAURENT</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO2004074466A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2004</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>DESLEE, DIDIER, LOUIS, ARTHUR</creatorcontrib><creatorcontrib>VOURC'H, CLAIRE, NADINE, MARIE</creatorcontrib><creatorcontrib>PERCHE, PIERRE-YVES</creatorcontrib><creatorcontrib>HELIOT, LAURENT</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>DESLEE, DIDIER, LOUIS, ARTHUR</au><au>VOURC'H, CLAIRE, NADINE, MARIE</au><au>PERCHE, PIERRE-YVES</au><au>HELIOT, LAURENT</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>NOVEL CELL LINE, PREPARATION METHOD THEREOF AND USE OF SAME, PARTICULARLY FOR MONITORING CHANGES IN CHROMATIN TEXTURE</title><date>2004-09-02</date><risdate>2004</risdate><abstract>The invention relates to cells from a stable cell line, e. g. monoclonal, having at least one inactive X chromosome, expressing a fusion protein comprising a protein which is associated with heterochromatin, such as a histone variant, and a fluorescent protein. The invention also relates to a method of preparing the stable cell line and to the use of said stable cell line cells for the screening of agents that can modify the texture of the chromatin and, in particular, the inactive state of the inactive X chromosome.
L'invention concerne des cellules de lignée cellulaire stable, notamment monoclonale, possédant au moins un chromosome X inactif, exprimant une protéine de fusion comprenant une protéine associé à l'hétérochromatine, notamment un variant d'histone, et une protéine fluorescente. L'invention concerne également un procédé de préparation de ladite lignée cellulaire stable et l'utilisation desdites cellules de lignée cellulaire stable pour cribler des agents susceptibles de modifier la texture de la chromatine et notamment l'état d'inactivation du chromosome X inactif.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | NOVEL CELL LINE, PREPARATION METHOD THEREOF AND USE OF SAME, PARTICULARLY FOR MONITORING CHANGES IN CHROMATIN TEXTURE |
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