METHODS FOR COMPARITIVE ANALYSIS OF CARBOHYDRATE POLYMERS AND CARBOHYDRATE POLYMERS IDENTIFIED USING SAME

Disclosed is a method for characterizing a carbohydrate polymer by identifying at least two binding agents that bind to the carbohydrate polymer. Binding is preferably determined by contacting the carbohydrate polymer with substrate that contains a plurality of first saccharide-binding agents affixe...

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Hauptverfasser: ROTHMAN, CHANA, AMOR, YEHUDIT, OR, EINAT, MARKMAN, OFER, ORAN, ASSAF
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Sprache:eng ; fre
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creator ROTHMAN, CHANA
AMOR, YEHUDIT
OR, EINAT
MARKMAN, OFER
ORAN, ASSAF
description Disclosed is a method for characterizing a carbohydrate polymer by identifying at least two binding agents that bind to the carbohydrate polymer. Binding is preferably determined by contacting the carbohydrate polymer with substrate that contains a plurality of first saccharide-binding agents affixed at predetermined locations on the substrate. The carbohydrate polymer is allowed to contact the substrate under conditions that allow for formation of a first complex between the first saccharide-binding agent and the carbohydrate polymer. A second saccharide-binding agent, which preferably includes a label, is also contacted with the carbohydrate polymer under conditions that allow for formation of a second complex between the second binding agent and the first complex. Identification of the first and second binding agent allows for characterization of the polysaccharide. L'invention concerne un procédé de caractérisation de polymère à base d'hydrates de carbone reposant sur l'identification d'au moins deux agents de liaison liés au polymère. De préférence, la liaison est déterminée par contact entre le polymère et un substrat renfermant des premiers agents de liaison de saccharide fixés en des points préétablis du substrat. Le polymère entre en contact avec le substrat dans des conditions qui permettent la formation d'un premier complexe entre le premier agent de liaison de saccharide et le polymère. Un second agent de liaison de saccharide, comprenant de préférence un marqueur, entre également en contact avec le polymère dans des conditions qui permettent la formation d'un second complexe entre le second agent de liaison et le premier complexe. L'identification des premier et second agents de liaison permet de caractériser le polysaccharide.
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De préférence, la liaison est déterminée par contact entre le polymère et un substrat renfermant des premiers agents de liaison de saccharide fixés en des points préétablis du substrat. Le polymère entre en contact avec le substrat dans des conditions qui permettent la formation d'un premier complexe entre le premier agent de liaison de saccharide et le polymère. Un second agent de liaison de saccharide, comprenant de préférence un marqueur, entre également en contact avec le polymère dans des conditions qui permettent la formation d'un second complexe entre le second agent de liaison et le premier complexe. L'identification des premier et second agents de liaison permet de caractériser le polysaccharide.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
title METHODS FOR COMPARITIVE ANALYSIS OF CARBOHYDRATE POLYMERS AND CARBOHYDRATE POLYMERS IDENTIFIED USING SAME
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