REVERSIBLY SOLUBLE ENZYME-POLYMER CONJUGATES
The present invention is directed to a method for producing reversibly soluble, catalytically active enzyme-polymer conjugates and the products thereof. In particular, the invention is directed to reversibly soluble catalytically active enzyme-polymer conjugates made by incorporating enzymes modifie...
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| creator | ILYASHENKO, VICTOR, M GOLOLOBOV, MIKHAIL, Y |
| description | The present invention is directed to a method for producing reversibly soluble, catalytically active enzyme-polymer conjugates and the products thereof. In particular, the invention is directed to reversibly soluble catalytically active enzyme-polymer conjugates made by incorporating enzymes modified to contain free vinyl double bonds into reversibly soluble polymers i.e., polymers that reversibly respond to slight changes in the environment, such as temperature, ionic strength, pH, electric fields, etc., during the polymerization reaction. Thus, when modified enzymes are incorporated into reversibly soluble polymers, the biocatalysts obtained can be precipitated without destroying the delicate enzyme(s). Moreover, these biocatalysts can be solubilized again and reused at the initial environmental conditions.
L'invention concerne un procédé de production de conjugués d'enzyme-polymère à solubilité réversible et actifs sur le plan catalytique, ainsi que les produits de ceux-ci. L'invention concerne notamment des conjugués d'enzyme-polymère à solubilité réversible et actifs sur le plan catalytique obtenus par incorporation d'enzymes modifiées, de manière à renfermer des doubles liaisons vinyliques libres dans des polymères à solubilité réversible, par exemple des polymères répondant réversiblement à de légers changements dans le milieu, tels que la température, la force ionique, le pH, les champs électriques, etc., pendant la réaction de polymérisation. Par conséquent, quand des enzymes modifiées sont incorporées dans des polymères à solubilité réversible, les biocatalyseurs obtenus peuvent être précipités sans détruire les enzymes délicates. De plus, ces biocatalyseurs peuvent être à nouveau solubilisés et réutilisés dans les conditions initiales du milieu. |
| format | Patent |
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L'invention concerne un procédé de production de conjugués d'enzyme-polymère à solubilité réversible et actifs sur le plan catalytique, ainsi que les produits de ceux-ci. L'invention concerne notamment des conjugués d'enzyme-polymère à solubilité réversible et actifs sur le plan catalytique obtenus par incorporation d'enzymes modifiées, de manière à renfermer des doubles liaisons vinyliques libres dans des polymères à solubilité réversible, par exemple des polymères répondant réversiblement à de légers changements dans le milieu, tels que la température, la force ionique, le pH, les champs électriques, etc., pendant la réaction de polymérisation. Par conséquent, quand des enzymes modifiées sont incorporées dans des polymères à solubilité réversible, les biocatalyseurs obtenus peuvent être précipités sans détruire les enzymes délicates. De plus, ces biocatalyseurs peuvent être à nouveau solubilisés et réutilisés dans les conditions initiales du milieu.</description><edition>7</edition><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC ; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TECHNICAL SUBJECTS COVERED BY FORMER USPC ; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS ; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS ; VINEGAR ; WINE</subject><creationdate>2003</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20030424&DB=EPODOC&CC=WO&NR=0234902A3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25544,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20030424&DB=EPODOC&CC=WO&NR=0234902A3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ILYASHENKO, VICTOR, M</creatorcontrib><creatorcontrib>GOLOLOBOV, MIKHAIL, Y</creatorcontrib><title>REVERSIBLY SOLUBLE ENZYME-POLYMER CONJUGATES</title><description>The present invention is directed to a method for producing reversibly soluble, catalytically active enzyme-polymer conjugates and the products thereof. In particular, the invention is directed to reversibly soluble catalytically active enzyme-polymer conjugates made by incorporating enzymes modified to contain free vinyl double bonds into reversibly soluble polymers i.e., polymers that reversibly respond to slight changes in the environment, such as temperature, ionic strength, pH, electric fields, etc., during the polymerization reaction. Thus, when modified enzymes are incorporated into reversibly soluble polymers, the biocatalysts obtained can be precipitated without destroying the delicate enzyme(s). Moreover, these biocatalysts can be solubilized again and reused at the initial environmental conditions.
L'invention concerne un procédé de production de conjugués d'enzyme-polymère à solubilité réversible et actifs sur le plan catalytique, ainsi que les produits de ceux-ci. L'invention concerne notamment des conjugués d'enzyme-polymère à solubilité réversible et actifs sur le plan catalytique obtenus par incorporation d'enzymes modifiées, de manière à renfermer des doubles liaisons vinyliques libres dans des polymères à solubilité réversible, par exemple des polymères répondant réversiblement à de légers changements dans le milieu, tels que la température, la force ionique, le pH, les champs électriques, etc., pendant la réaction de polymérisation. Par conséquent, quand des enzymes modifiées sont incorporées dans des polymères à solubilité réversible, les biocatalyseurs obtenus peuvent être précipités sans détruire les enzymes délicates. De plus, ces biocatalyseurs peuvent être à nouveau solubilisés et réutilisés dans les conditions initiales du milieu.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC</subject><subject>GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TECHNICAL SUBJECTS COVERED BY FORMER USPC</subject><subject>TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS</subject><subject>TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2003</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNAJcg1zDQr2dPKJVAj29wl18nFVcPWLivR11Q3w9wFSQQrO_n5eoe6OIa7BPAysaYk5xam8UJqbQcHNNcTZQze1ID8-tbggMTk1L7UkPtzfwMjYxNLAyNHYmAglAI2VJWY</recordid><startdate>20030424</startdate><enddate>20030424</enddate><creator>ILYASHENKO, VICTOR, M</creator><creator>GOLOLOBOV, MIKHAIL, Y</creator><scope>EVB</scope></search><sort><creationdate>20030424</creationdate><title>REVERSIBLY SOLUBLE ENZYME-POLYMER CONJUGATES</title><author>ILYASHENKO, VICTOR, M ; GOLOLOBOV, MIKHAIL, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO0234902A33</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2003</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC</topic><topic>GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TECHNICAL SUBJECTS COVERED BY FORMER USPC</topic><topic>TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS</topic><topic>TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ILYASHENKO, VICTOR, M</creatorcontrib><creatorcontrib>GOLOLOBOV, MIKHAIL, Y</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ILYASHENKO, VICTOR, M</au><au>GOLOLOBOV, MIKHAIL, Y</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>REVERSIBLY SOLUBLE ENZYME-POLYMER CONJUGATES</title><date>2003-04-24</date><risdate>2003</risdate><abstract>The present invention is directed to a method for producing reversibly soluble, catalytically active enzyme-polymer conjugates and the products thereof. In particular, the invention is directed to reversibly soluble catalytically active enzyme-polymer conjugates made by incorporating enzymes modified to contain free vinyl double bonds into reversibly soluble polymers i.e., polymers that reversibly respond to slight changes in the environment, such as temperature, ionic strength, pH, electric fields, etc., during the polymerization reaction. Thus, when modified enzymes are incorporated into reversibly soluble polymers, the biocatalysts obtained can be precipitated without destroying the delicate enzyme(s). Moreover, these biocatalysts can be solubilized again and reused at the initial environmental conditions.
L'invention concerne un procédé de production de conjugués d'enzyme-polymère à solubilité réversible et actifs sur le plan catalytique, ainsi que les produits de ceux-ci. L'invention concerne notamment des conjugués d'enzyme-polymère à solubilité réversible et actifs sur le plan catalytique obtenus par incorporation d'enzymes modifiées, de manière à renfermer des doubles liaisons vinyliques libres dans des polymères à solubilité réversible, par exemple des polymères répondant réversiblement à de légers changements dans le milieu, tels que la température, la force ionique, le pH, les champs électriques, etc., pendant la réaction de polymérisation. Par conséquent, quand des enzymes modifiées sont incorporées dans des polymères à solubilité réversible, les biocatalyseurs obtenus peuvent être précipités sans détruire les enzymes délicates. De plus, ces biocatalyseurs peuvent être à nouveau solubilisés et réutilisés dans les conditions initiales du milieu.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS VINEGAR WINE |
| title | REVERSIBLY SOLUBLE ENZYME-POLYMER CONJUGATES |
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