METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES
A method of providing polypeptide preparations having a reduced content of undesired enzymatic side activities, the method comprising subjecting a medium containing a desired polypeptide such as an enzyme, a pharmaceutically active or immunologically active polypeptide to a pH of less than 2 for a p...
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| creator | HARBOE, MARIANNE, KIRSTEN |
| description | A method of providing polypeptide preparations having a reduced content of undesired enzymatic side activities, the method comprising subjecting a medium containing a desired polypeptide such as an enzyme, a pharmaceutically active or immunologically active polypeptide to a pH of less than 2 for a period of time that is required to inactivate the side activities whilst retaining the activity of the desired polypeptide. The method is useful for providing milk clotting enzyme products including rennets or coagulants based on chymosin or pepsin or microbial aspartic proteases e.g. derived from bacterial species and species of filamentous fungi.
La présente invention concerne une technique de préparations de polypeptide à activités secondaires enzymatiques indésirables réduites. Cette technique consiste à soumettre un milieu contenant un polypeptide recherché tel qu'une enzyme, un polypeptide pharmaceutiquement actif ou un polypeptide immunologiquement actif à un pH inférieur à 2 pendant la durée requise pour inactiver les activités secondaires tout en conservant l'activité du polypeptide recherché. Cette technique convient pour obtenir des produits enzymatiques pour le caillage du lait comprenant des présures et des coagulants à base de rennine, de pepsine ou de protéases aspartiques microbiennes, dérivées par exemple d'espèces bactériennes et d'espèces de champignons filamenteux. |
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La présente invention concerne une technique de préparations de polypeptide à activités secondaires enzymatiques indésirables réduites. Cette technique consiste à soumettre un milieu contenant un polypeptide recherché tel qu'une enzyme, un polypeptide pharmaceutiquement actif ou un polypeptide immunologiquement actif à un pH inférieur à 2 pendant la durée requise pour inactiver les activités secondaires tout en conservant l'activité du polypeptide recherché. Cette technique convient pour obtenir des produits enzymatiques pour le caillage du lait comprenant des présures et des coagulants à base de rennine, de pepsine ou de protéases aspartiques microbiennes, dérivées par exemple d'espèces bactériennes et d'espèces de champignons filamenteux.</description><edition>7</edition><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2004</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20040429&DB=EPODOC&CC=WO&NR=02064759A8$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20040429&DB=EPODOC&CC=WO&NR=02064759A8$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HARBOE, MARIANNE, KIRSTEN</creatorcontrib><title>METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES</title><description>A method of providing polypeptide preparations having a reduced content of undesired enzymatic side activities, the method comprising subjecting a medium containing a desired polypeptide such as an enzyme, a pharmaceutically active or immunologically active polypeptide to a pH of less than 2 for a period of time that is required to inactivate the side activities whilst retaining the activity of the desired polypeptide. The method is useful for providing milk clotting enzyme products including rennets or coagulants based on chymosin or pepsin or microbial aspartic proteases e.g. derived from bacterial species and species of filamentous fungi.
La présente invention concerne une technique de préparations de polypeptide à activités secondaires enzymatiques indésirables réduites. Cette technique consiste à soumettre un milieu contenant un polypeptide recherché tel qu'une enzyme, un polypeptide pharmaceutiquement actif ou un polypeptide immunologiquement actif à un pH inférieur à 2 pendant la durée requise pour inactiver les activités secondaires tout en conservant l'activité du polypeptide recherché. Cette technique convient pour obtenir des produits enzymatiques pour le caillage du lait comprenant des présures et des coagulants à base de rennine, de pepsine ou de protéases aspartiques microbiennes, dérivées par exemple d'espèces bactériennes et d'espèces de champignons filamenteux.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2004</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZAj2dQ3x8HdR8HdTCAjyD_N08fRzVwjw94kMcA0I8XRxBYq6BjgGOYZ4-vsFK4R7hngoBLm6hDq7uii4-kVF-gIlnBWCQQodnUM8wzxDPF2DeRhY0xJzilN5oTQ3g6Kba4izh25qQX58anFBYnJqXmpJfLi_gZGBmYm5qaWjhTExagBBxzCL</recordid><startdate>20040429</startdate><enddate>20040429</enddate><creator>HARBOE, MARIANNE, KIRSTEN</creator><scope>EVB</scope></search><sort><creationdate>20040429</creationdate><title>METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES</title><author>HARBOE, MARIANNE, KIRSTEN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_WO02064759A83</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre</language><creationdate>2004</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HARBOE, MARIANNE, KIRSTEN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HARBOE, MARIANNE, KIRSTEN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES</title><date>2004-04-29</date><risdate>2004</risdate><abstract>A method of providing polypeptide preparations having a reduced content of undesired enzymatic side activities, the method comprising subjecting a medium containing a desired polypeptide such as an enzyme, a pharmaceutically active or immunologically active polypeptide to a pH of less than 2 for a period of time that is required to inactivate the side activities whilst retaining the activity of the desired polypeptide. The method is useful for providing milk clotting enzyme products including rennets or coagulants based on chymosin or pepsin or microbial aspartic proteases e.g. derived from bacterial species and species of filamentous fungi.
La présente invention concerne une technique de préparations de polypeptide à activités secondaires enzymatiques indésirables réduites. Cette technique consiste à soumettre un milieu contenant un polypeptide recherché tel qu'une enzyme, un polypeptide pharmaceutiquement actif ou un polypeptide immunologiquement actif à un pH inférieur à 2 pendant la durée requise pour inactiver les activités secondaires tout en conservant l'activité du polypeptide recherché. Cette technique convient pour obtenir des produits enzymatiques pour le caillage du lait comprenant des présures et des coagulants à base de rennine, de pepsine ou de protéases aspartiques microbiennes, dérivées par exemple d'espèces bactériennes et d'espèces de champignons filamenteux.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD OF PROVIDING POLYPEPTIDE PREPARATIONS WITH REDUCED ENZYMATIC SIDE ACTIVITIES |
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