METHOD FOR IMMOBILIZING A NOVEL PENICILLIN V ACYLASE OF STREPTOMYCES LAVENDULAE FOR OBTAINING 6-AMINO PENICILLANIC ACID

Immobilization of penicillin V acylase of Streptomyces lavendulae ATCC 13664 on commercial support Eupergit C has made it possible to obtain biocatalyst ECPVA, a novel acylase immobilized penicillin for obtaining 6-APA from penicillin V. The invention describes the method for conducting said immobil...

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Hauptverfasser:	DE LA MATA RIESCO, ISABEL, ACEBAL SARABIA, CARMEN, CASTILLON BORREGUERO, MARIA PILAR, TORRES BACETE, JESUS, TORRES GUZMAN, RAQUEL, ARROYO SANCHEZ, MIGUEL
Format:	Patent
Sprache:	eng ; fre ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Immobilization of penicillin V acylase of Streptomyces lavendulae ATCC 13664 on commercial support Eupergit C has made it possible to obtain biocatalyst ECPVA, a novel acylase immobilized penicillin for obtaining 6-APA from penicillin V. The invention describes the method for conducting said immobilization and the conditions for the reaction which make it possible to hydrolyze penicillin V catalyzed by ECPVA. La inmovilización de la penicilina V acilasa de Streptomyces lavendulae ATCC 13664 en el soporte comercial Eupergit C ha permitido la obtención del biocatalizador ECPVA, una nueva penicilina acilasa inmovilizada para la obtención de 6-APA a partir de penicilina V. Se describe el método para llevar a cabo esta inmovilización y las condiciones de la reacción que hacen posible la hidrólisis de penicilina V catalizada por ECPVA. L'immobilisation de la pénicilline V acylase de Streptomyces lavendulae ATCC 13664 sur le support commercial Eupergit C a permis d'obtenir le biocatalyseur ECPVA, une nouvelle pénicilline V acylase immobilisée en vue de l'obtention de 6-APA à partir de la pénicilline V. L'invention concerne le procédé permettant de réaliser cette immobilisation dans les conditions de réaction qui rendent possible l'hydrolyse de pénicilline V catalysée par ECPVA.