Facile laboratory method for localising biomolecules to the surface of cells and viruses

Facile laboratory method for localising biomolecules to the surface of cells and viruses

Method of localizing a sulfhydryl (-SH) group containing biomolecule to the surface of a cell membrane by mixing in a volatile reaction buffer a molar excess of the sulfhydryl (-SH) group containing biomolecule with a lipid conjugated maleimide of the structure F-S-L as defined in the specification...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Bovin Nikolai Vladimirovich, Henry Stephen Michael, Rodionov Igor Leonidovich
Format: Patent
Sprache:eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator Bovin Nikolai Vladimirovich
Henry Stephen Michael
Rodionov Igor Leonidovich
description Method of localizing a sulfhydryl (-SH) group containing biomolecule to the surface of a cell membrane by mixing in a volatile reaction buffer a molar excess of the sulfhydryl (-SH) group containing biomolecule with a lipid conjugated maleimide of the structure F-S-L as defined in the specification to provide a reaction mix, incubating the reaction mix for a time and at a temperature sufficient to allow all the lipid conjugated maleimide to have reacted with the sulfhydryl (-SH) group, and freeze-drying the reaction mix to remove the volatile reaction buffer and provide a reaction product. An aqueous solution of the reaction product is contacted with the cell membrane.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US9915653B2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US9915653B2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US9915653B23</originalsourceid><addsrcrecordid>eNqNyzEKwkAQRuE0FqLe4b-AhYYIaRWDvQp2YbKZNQuTTNjZFby9Fh7A6jXfWxaPhlwQhlCnkZLGN0ZOg_bwGiHqSIKF6Yku6KjCLgsbkiINDMvRk2Ooh2MRA009XiFmY1sXC09ivPl1VaA5306XLc_ass3fb-LU3q91vasOVXncl3-QD0wpOkk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Facile laboratory method for localising biomolecules to the surface of cells and viruses</title><source>esp@cenet</source><creator>Bovin Nikolai Vladimirovich ; Henry Stephen Michael ; Rodionov Igor Leonidovich</creator><creatorcontrib>Bovin Nikolai Vladimirovich ; Henry Stephen Michael ; Rodionov Igor Leonidovich</creatorcontrib><description>Method of localizing a sulfhydryl (-SH) group containing biomolecule to the surface of a cell membrane by mixing in a volatile reaction buffer a molar excess of the sulfhydryl (-SH) group containing biomolecule with a lipid conjugated maleimide of the structure F-S-L as defined in the specification to provide a reaction mix, incubating the reaction mix for a time and at a temperature sufficient to allow all the lipid conjugated maleimide to have reacted with the sulfhydryl (-SH) group, and freeze-drying the reaction mix to remove the volatile reaction buffer and provide a reaction product. An aqueous solution of the reaction product is contacted with the cell membrane.</description><language>eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2018</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20180313&amp;DB=EPODOC&amp;CC=US&amp;NR=9915653B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25547,76298</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20180313&amp;DB=EPODOC&amp;CC=US&amp;NR=9915653B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Bovin Nikolai Vladimirovich</creatorcontrib><creatorcontrib>Henry Stephen Michael</creatorcontrib><creatorcontrib>Rodionov Igor Leonidovich</creatorcontrib><title>Facile laboratory method for localising biomolecules to the surface of cells and viruses</title><description>Method of localizing a sulfhydryl (-SH) group containing biomolecule to the surface of a cell membrane by mixing in a volatile reaction buffer a molar excess of the sulfhydryl (-SH) group containing biomolecule with a lipid conjugated maleimide of the structure F-S-L as defined in the specification to provide a reaction mix, incubating the reaction mix for a time and at a temperature sufficient to allow all the lipid conjugated maleimide to have reacted with the sulfhydryl (-SH) group, and freeze-drying the reaction mix to remove the volatile reaction buffer and provide a reaction product. An aqueous solution of the reaction product is contacted with the cell membrane.</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2018</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyzEKwkAQRuE0FqLe4b-AhYYIaRWDvQp2YbKZNQuTTNjZFby9Fh7A6jXfWxaPhlwQhlCnkZLGN0ZOg_bwGiHqSIKF6Yku6KjCLgsbkiINDMvRk2Ooh2MRA009XiFmY1sXC09ivPl1VaA5306XLc_ass3fb-LU3q91vasOVXncl3-QD0wpOkk</recordid><startdate>20180313</startdate><enddate>20180313</enddate><creator>Bovin Nikolai Vladimirovich</creator><creator>Henry Stephen Michael</creator><creator>Rodionov Igor Leonidovich</creator><scope>EVB</scope></search><sort><creationdate>20180313</creationdate><title>Facile laboratory method for localising biomolecules to the surface of cells and viruses</title><author>Bovin Nikolai Vladimirovich ; Henry Stephen Michael ; Rodionov Igor Leonidovich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US9915653B23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2018</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>Bovin Nikolai Vladimirovich</creatorcontrib><creatorcontrib>Henry Stephen Michael</creatorcontrib><creatorcontrib>Rodionov Igor Leonidovich</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Bovin Nikolai Vladimirovich</au><au>Henry Stephen Michael</au><au>Rodionov Igor Leonidovich</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Facile laboratory method for localising biomolecules to the surface of cells and viruses</title><date>2018-03-13</date><risdate>2018</risdate><abstract>Method of localizing a sulfhydryl (-SH) group containing biomolecule to the surface of a cell membrane by mixing in a volatile reaction buffer a molar excess of the sulfhydryl (-SH) group containing biomolecule with a lipid conjugated maleimide of the structure F-S-L as defined in the specification to provide a reaction mix, incubating the reaction mix for a time and at a temperature sufficient to allow all the lipid conjugated maleimide to have reacted with the sulfhydryl (-SH) group, and freeze-drying the reaction mix to remove the volatile reaction buffer and provide a reaction product. An aqueous solution of the reaction product is contacted with the cell membrane.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng
recordid cdi_epo_espacenet_US9915653B2
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Facile laboratory method for localising biomolecules to the surface of cells and viruses
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T04%3A09%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=Bovin%20Nikolai%20Vladimirovich&rft.date=2018-03-13&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS9915653B2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true