Method for purification of nucleic acids, particularly from fixed tissue

Method for purification of nucleic acids, particularly from fixed tissue

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommoda...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: HENNIG GUIDO, EUTING HEIKE, IZMAILOV ALEXANDRE
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
DERIVATIVES THEREOF
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SUGARS
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator HENNIG GUIDO
EUTING HEIKE
IZMAILOV ALEXANDRE
description The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US8703931B2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US8703931B2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US8703931B23</originalsourceid><addsrcrecordid>eNqNyj0OQUEQAOBtFII7zAFIsAVaQl6jQv0ymZ2JSdbOZn8Sbq9xANXXfFM3XLk9LYBYgdyLihI2tQQmkDpFVgIkDXUJGUtT6hFL_IAUe4HomwM0rbXz3E0EY-XFz5mDy_l-GlacbeSakThxGx-3_W7tD35z3Po_yhdO7zQ5</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Method for purification of nucleic acids, particularly from fixed tissue</title><source>esp@cenet</source><creator>HENNIG GUIDO ; EUTING HEIKE ; IZMAILOV ALEXANDRE</creator><creatorcontrib>HENNIG GUIDO ; EUTING HEIKE ; IZMAILOV ALEXANDRE</creatorcontrib><description>The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.</description><language>eng</language><subject>CHEMISTRY ; DERIVATIVES THEREOF ; METALLURGY ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ORGANIC CHEMISTRY ; SUGARS</subject><creationdate>2014</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20140422&amp;DB=EPODOC&amp;CC=US&amp;NR=8703931B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20140422&amp;DB=EPODOC&amp;CC=US&amp;NR=8703931B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HENNIG GUIDO</creatorcontrib><creatorcontrib>EUTING HEIKE</creatorcontrib><creatorcontrib>IZMAILOV ALEXANDRE</creatorcontrib><title>Method for purification of nucleic acids, particularly from fixed tissue</title><description>The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.</description><subject>CHEMISTRY</subject><subject>DERIVATIVES THEREOF</subject><subject>METALLURGY</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOSIDES</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC CHEMISTRY</subject><subject>SUGARS</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2014</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyj0OQUEQAOBtFII7zAFIsAVaQl6jQv0ymZ2JSdbOZn8Sbq9xANXXfFM3XLk9LYBYgdyLihI2tQQmkDpFVgIkDXUJGUtT6hFL_IAUe4HomwM0rbXz3E0EY-XFz5mDy_l-GlacbeSakThxGx-3_W7tD35z3Po_yhdO7zQ5</recordid><startdate>20140422</startdate><enddate>20140422</enddate><creator>HENNIG GUIDO</creator><creator>EUTING HEIKE</creator><creator>IZMAILOV ALEXANDRE</creator><scope>EVB</scope></search><sort><creationdate>20140422</creationdate><title>Method for purification of nucleic acids, particularly from fixed tissue</title><author>HENNIG GUIDO ; EUTING HEIKE ; IZMAILOV ALEXANDRE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US8703931B23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2014</creationdate><topic>CHEMISTRY</topic><topic>DERIVATIVES THEREOF</topic><topic>METALLURGY</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOSIDES</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC CHEMISTRY</topic><topic>SUGARS</topic><toplevel>online_resources</toplevel><creatorcontrib>HENNIG GUIDO</creatorcontrib><creatorcontrib>EUTING HEIKE</creatorcontrib><creatorcontrib>IZMAILOV ALEXANDRE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HENNIG GUIDO</au><au>EUTING HEIKE</au><au>IZMAILOV ALEXANDRE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for purification of nucleic acids, particularly from fixed tissue</title><date>2014-04-22</date><risdate>2014</risdate><abstract>The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng
recordid cdi_epo_espacenet_US8703931B2
source esp@cenet
subjects CHEMISTRY
DERIVATIVES THEREOF
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SUGARS
title Method for purification of nucleic acids, particularly from fixed tissue
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T10%3A44%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=HENNIG%20GUIDO&rft.date=2014-04-22&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS8703931B2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true