Method for determining physiological state of microbial community and wastewater treatment method

The present invention provides a method for determining the physiological state of a microbial community, which rapidly and precisely determines the physiological state of a microbial community present in a treatment tank of a wastewater biological treatment facility; and a wastewater treatment meth...

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Hauptverfasser: IGARASHI RYOJI, YAMAMOTO TAKUJI
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creator IGARASHI RYOJI
YAMAMOTO TAKUJI
description The present invention provides a method for determining the physiological state of a microbial community, which rapidly and precisely determines the physiological state of a microbial community present in a treatment tank of a wastewater biological treatment facility; and a wastewater treatment method utilizing the same. In the method for determining the physiological state of a microbial community of the present invention, the method includes the steps of staining the microbial community collected from a treatment tank of a wastewater biological treatment facility with a first fluorescent dye which binds to a nucleotide chain of a microbe in the microbial community, and a second fluorescent dye which is degraded by an enzyme in cells of the microbe to emit fluorescent light having a wavelength different from that of the first fluorescent dye; measuring a first fluorescent intensity (F1) derived from the first fluorescent dye and a second fluorescent intensity (F2) derived from the second fluorescent dye, regarding the stained microbial community; and determining the satisfactory degree of the physiological state of the microbial community.
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In the method for determining the physiological state of a microbial community of the present invention, the method includes the steps of staining the microbial community collected from a treatment tank of a wastewater biological treatment facility with a first fluorescent dye which binds to a nucleotide chain of a microbe in the microbial community, and a second fluorescent dye which is degraded by an enzyme in cells of the microbe to emit fluorescent light having a wavelength different from that of the first fluorescent dye; measuring a first fluorescent intensity (F1) derived from the first fluorescent dye and a second fluorescent intensity (F2) derived from the second fluorescent dye, regarding the stained microbial community; and determining the satisfactory degree of the physiological state of the microbial community.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SEPARATION ; SPIRITS ; TESTING ; TRANSPORTING ; TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE ; VINEGAR ; WINE</subject><creationdate>2014</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20140225&amp;DB=EPODOC&amp;CC=US&amp;NR=8658037B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20140225&amp;DB=EPODOC&amp;CC=US&amp;NR=8658037B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>IGARASHI RYOJI</creatorcontrib><creatorcontrib>YAMAMOTO TAKUJI</creatorcontrib><title>Method for determining physiological state of microbial community and wastewater treatment method</title><description>The present invention provides a method for determining the physiological state of a microbial community, which rapidly and precisely determines the physiological state of a microbial community present in a treatment tank of a wastewater biological treatment facility; and a wastewater treatment method utilizing the same. 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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SEPARATION
SPIRITS
TESTING
TRANSPORTING
TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
VINEGAR
WINE
title Method for determining physiological state of microbial community and wastewater treatment method
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