Thermostable biocatalyst combination for nucleoside synthesis
The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more c...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | LOPEZ GOMEZ CRISTINA MONTILLA AREVALO RAFAEL ESTEVEZ COMPANY CARLOS PASCUAL GILABERT MARTA DERONCELE THOMAS VICTOR MANUEL CASTELLS BOLIART JOSEP |
| description | The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PN-Pase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US8512997B2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US8512997B2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US8512997B23</originalsourceid><addsrcrecordid>eNrjZLANyUgtys0vLklMyklVSMrMT04sScypLC5RSM7PTcrMSyzJzM9TSMsvUsgrTc5JzS_OTElVKK7MK8lILc4s5mFgTUvMKU7lhdLcDApuriHOHrqpBfnxqcUFicmpeakl8aHBFqaGRpaW5k5GxkQoAQBGRjEg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Thermostable biocatalyst combination for nucleoside synthesis</title><source>esp@cenet</source><creator>LOPEZ GOMEZ CRISTINA ; MONTILLA AREVALO RAFAEL ; ESTEVEZ COMPANY CARLOS ; PASCUAL GILABERT MARTA ; DERONCELE THOMAS VICTOR MANUEL ; CASTELLS BOLIART JOSEP</creator><creatorcontrib>LOPEZ GOMEZ CRISTINA ; MONTILLA AREVALO RAFAEL ; ESTEVEZ COMPANY CARLOS ; PASCUAL GILABERT MARTA ; DERONCELE THOMAS VICTOR MANUEL ; CASTELLS BOLIART JOSEP</creatorcontrib><description>The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PN-Pase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2013</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20130820&DB=EPODOC&CC=US&NR=8512997B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76418</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20130820&DB=EPODOC&CC=US&NR=8512997B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LOPEZ GOMEZ CRISTINA</creatorcontrib><creatorcontrib>MONTILLA AREVALO RAFAEL</creatorcontrib><creatorcontrib>ESTEVEZ COMPANY CARLOS</creatorcontrib><creatorcontrib>PASCUAL GILABERT MARTA</creatorcontrib><creatorcontrib>DERONCELE THOMAS VICTOR MANUEL</creatorcontrib><creatorcontrib>CASTELLS BOLIART JOSEP</creatorcontrib><title>Thermostable biocatalyst combination for nucleoside synthesis</title><description>The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PN-Pase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2013</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLANyUgtys0vLklMyklVSMrMT04sScypLC5RSM7PTcrMSyzJzM9TSMsvUsgrTc5JzS_OTElVKK7MK8lILc4s5mFgTUvMKU7lhdLcDApuriHOHrqpBfnxqcUFicmpeakl8aHBFqaGRpaW5k5GxkQoAQBGRjEg</recordid><startdate>20130820</startdate><enddate>20130820</enddate><creator>LOPEZ GOMEZ CRISTINA</creator><creator>MONTILLA AREVALO RAFAEL</creator><creator>ESTEVEZ COMPANY CARLOS</creator><creator>PASCUAL GILABERT MARTA</creator><creator>DERONCELE THOMAS VICTOR MANUEL</creator><creator>CASTELLS BOLIART JOSEP</creator><scope>EVB</scope></search><sort><creationdate>20130820</creationdate><title>Thermostable biocatalyst combination for nucleoside synthesis</title><author>LOPEZ GOMEZ CRISTINA ; MONTILLA AREVALO RAFAEL ; ESTEVEZ COMPANY CARLOS ; PASCUAL GILABERT MARTA ; DERONCELE THOMAS VICTOR MANUEL ; CASTELLS BOLIART JOSEP</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US8512997B23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2013</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LOPEZ GOMEZ CRISTINA</creatorcontrib><creatorcontrib>MONTILLA AREVALO RAFAEL</creatorcontrib><creatorcontrib>ESTEVEZ COMPANY CARLOS</creatorcontrib><creatorcontrib>PASCUAL GILABERT MARTA</creatorcontrib><creatorcontrib>DERONCELE THOMAS VICTOR MANUEL</creatorcontrib><creatorcontrib>CASTELLS BOLIART JOSEP</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LOPEZ GOMEZ CRISTINA</au><au>MONTILLA AREVALO RAFAEL</au><au>ESTEVEZ COMPANY CARLOS</au><au>PASCUAL GILABERT MARTA</au><au>DERONCELE THOMAS VICTOR MANUEL</au><au>CASTELLS BOLIART JOSEP</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Thermostable biocatalyst combination for nucleoside synthesis</title><date>2013-08-20</date><risdate>2013</risdate><abstract>The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PN-Pase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_US8512997B2 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Thermostable biocatalyst combination for nucleoside synthesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T16%3A52%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LOPEZ%20GOMEZ%20CRISTINA&rft.date=2013-08-20&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS8512997B2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |