Methods for protein refolding

The present invention discloses improved methods of disaggregating protein aggregates, and refolding denatured proteins, using high pressure. In particular, the present invention provides for the use of agitation, high temperature, stepped depressurization, dialysis and dilution under pressure to in...

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Bibliographische Detailangaben
Hauptverfasser:	RANDOLPH THEODORE W, WEBB JONATHAN N, CARPENTER JOHN F, ST. JOHN RICHARD J
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention discloses improved methods of disaggregating protein aggregates, and refolding denatured proteins, using high pressure. In particular, the present invention provides for the use of agitation, high temperature, stepped depressurization, dialysis and dilution under pressure to increase the speed and extent of aggregate dissolution and protein refolding.