DNA sequence encoding penicillin acylase, novel recombinant recombinant DNA constructs and recombinant microorganisms
The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from Achromobacter sp. CCM 4824 (formerly Comamonas testosteroni CCM 4824), eventually of the fragments of t...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | STEPANEK VACLAV HOLLEROVA LENKA PLHACKOVA KAMILA ANUPAMA DATLA MARSALEK JAROSLAV BECKA STANISLAV KYSLIK PAVEL RAJASEKAR VYASARAYANI WILLIAMS |
| description | The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from Achromobacter sp. CCM 4824 (formerly Comamonas testosteroni CCM 4824), eventually of the fragments of this sequence having the length of at least 150 nucleotides. The sequence can be used in the formation of a DNA construct, eventually the construct having at least one regulatory sequence regulating the expression of the gene and the production of a polypeptide with the penicillin acylase activity. The sequence can form part of a recombinant expression vector, which consists of the above-mentioned construct, promoter, translational start signal, translational and transcriptional stop signal. Further, the invention concerns a recombinant host cell, containing the nucleic acid construct carried by the vector or integrated into the cell chromosome, and the E. coli BL21 strain containing said sequence of the nucleotides encoding the penicillin acylase carried in the pKXIP1, the pKLP3 or the pKLP6 plasmid. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US8039604B2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US8039604B2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US8039604B23</originalsourceid><addsrcrecordid>eNqNjL0KwlAMhbs4iPoOeQCFYkV09BcnF3UuMY0lcJvUm1vBt1fBxc3lnA_Ox-ln3fa4Aud7x0oM77BKtIaWVUhCEAWkZ0DnMag9OEBksuYqipp--PNDpp5iR8kBtfqZG6FoFmtU8caHWe-GwXn07UEG-915c5hwayV7i8TKqbycFnmxnOez9bT4Q3kBaMhFdw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>DNA sequence encoding penicillin acylase, novel recombinant recombinant DNA constructs and recombinant microorganisms</title><source>esp@cenet</source><creator>STEPANEK VACLAV ; HOLLEROVA LENKA ; PLHACKOVA KAMILA ; ANUPAMA DATLA ; MARSALEK JAROSLAV ; BECKA STANISLAV ; KYSLIK PAVEL ; RAJASEKAR VYASARAYANI WILLIAMS</creator><creatorcontrib>STEPANEK VACLAV ; HOLLEROVA LENKA ; PLHACKOVA KAMILA ; ANUPAMA DATLA ; MARSALEK JAROSLAV ; BECKA STANISLAV ; KYSLIK PAVEL ; RAJASEKAR VYASARAYANI WILLIAMS</creatorcontrib><description>The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from Achromobacter sp. CCM 4824 (formerly Comamonas testosteroni CCM 4824), eventually of the fragments of this sequence having the length of at least 150 nucleotides. The sequence can be used in the formation of a DNA construct, eventually the construct having at least one regulatory sequence regulating the expression of the gene and the production of a polypeptide with the penicillin acylase activity. The sequence can form part of a recombinant expression vector, which consists of the above-mentioned construct, promoter, translational start signal, translational and transcriptional stop signal. Further, the invention concerns a recombinant host cell, containing the nucleic acid construct carried by the vector or integrated into the cell chromosome, and the E. coli BL21 strain containing said sequence of the nucleotides encoding the penicillin acylase carried in the pKXIP1, the pKLP3 or the pKLP6 plasmid.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; DERIVATIVES THEREOF ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ORGANIC CHEMISTRY ; SPIRITS ; SUGARS ; VINEGAR ; WINE</subject><creationdate>2011</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20111018&DB=EPODOC&CC=US&NR=8039604B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20111018&DB=EPODOC&CC=US&NR=8039604B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>STEPANEK VACLAV</creatorcontrib><creatorcontrib>HOLLEROVA LENKA</creatorcontrib><creatorcontrib>PLHACKOVA KAMILA</creatorcontrib><creatorcontrib>ANUPAMA DATLA</creatorcontrib><creatorcontrib>MARSALEK JAROSLAV</creatorcontrib><creatorcontrib>BECKA STANISLAV</creatorcontrib><creatorcontrib>KYSLIK PAVEL</creatorcontrib><creatorcontrib>RAJASEKAR VYASARAYANI WILLIAMS</creatorcontrib><title>DNA sequence encoding penicillin acylase, novel recombinant recombinant DNA constructs and recombinant microorganisms</title><description>The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from Achromobacter sp. CCM 4824 (formerly Comamonas testosteroni CCM 4824), eventually of the fragments of this sequence having the length of at least 150 nucleotides. The sequence can be used in the formation of a DNA construct, eventually the construct having at least one regulatory sequence regulating the expression of the gene and the production of a polypeptide with the penicillin acylase activity. The sequence can form part of a recombinant expression vector, which consists of the above-mentioned construct, promoter, translational start signal, translational and transcriptional stop signal. Further, the invention concerns a recombinant host cell, containing the nucleic acid construct carried by the vector or integrated into the cell chromosome, and the E. coli BL21 strain containing said sequence of the nucleotides encoding the penicillin acylase carried in the pKXIP1, the pKLP3 or the pKLP6 plasmid.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>DERIVATIVES THEREOF</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOSIDES</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC CHEMISTRY</subject><subject>SPIRITS</subject><subject>SUGARS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2011</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjL0KwlAMhbs4iPoOeQCFYkV09BcnF3UuMY0lcJvUm1vBt1fBxc3lnA_Ox-ln3fa4Aud7x0oM77BKtIaWVUhCEAWkZ0DnMag9OEBksuYqipp--PNDpp5iR8kBtfqZG6FoFmtU8caHWe-GwXn07UEG-915c5hwayV7i8TKqbycFnmxnOez9bT4Q3kBaMhFdw</recordid><startdate>20111018</startdate><enddate>20111018</enddate><creator>STEPANEK VACLAV</creator><creator>HOLLEROVA LENKA</creator><creator>PLHACKOVA KAMILA</creator><creator>ANUPAMA DATLA</creator><creator>MARSALEK JAROSLAV</creator><creator>BECKA STANISLAV</creator><creator>KYSLIK PAVEL</creator><creator>RAJASEKAR VYASARAYANI WILLIAMS</creator><scope>EVB</scope></search><sort><creationdate>20111018</creationdate><title>DNA sequence encoding penicillin acylase, novel recombinant recombinant DNA constructs and recombinant microorganisms</title><author>STEPANEK VACLAV ; HOLLEROVA LENKA ; PLHACKOVA KAMILA ; ANUPAMA DATLA ; MARSALEK JAROSLAV ; BECKA STANISLAV ; KYSLIK PAVEL ; RAJASEKAR VYASARAYANI WILLIAMS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US8039604B23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2011</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>DERIVATIVES THEREOF</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOSIDES</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC CHEMISTRY</topic><topic>SPIRITS</topic><topic>SUGARS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>STEPANEK VACLAV</creatorcontrib><creatorcontrib>HOLLEROVA LENKA</creatorcontrib><creatorcontrib>PLHACKOVA KAMILA</creatorcontrib><creatorcontrib>ANUPAMA DATLA</creatorcontrib><creatorcontrib>MARSALEK JAROSLAV</creatorcontrib><creatorcontrib>BECKA STANISLAV</creatorcontrib><creatorcontrib>KYSLIK PAVEL</creatorcontrib><creatorcontrib>RAJASEKAR VYASARAYANI WILLIAMS</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>STEPANEK VACLAV</au><au>HOLLEROVA LENKA</au><au>PLHACKOVA KAMILA</au><au>ANUPAMA DATLA</au><au>MARSALEK JAROSLAV</au><au>BECKA STANISLAV</au><au>KYSLIK PAVEL</au><au>RAJASEKAR VYASARAYANI WILLIAMS</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>DNA sequence encoding penicillin acylase, novel recombinant recombinant DNA constructs and recombinant microorganisms</title><date>2011-10-18</date><risdate>2011</risdate><abstract>The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from Achromobacter sp. CCM 4824 (formerly Comamonas testosteroni CCM 4824), eventually of the fragments of this sequence having the length of at least 150 nucleotides. The sequence can be used in the formation of a DNA construct, eventually the construct having at least one regulatory sequence regulating the expression of the gene and the production of a polypeptide with the penicillin acylase activity. The sequence can form part of a recombinant expression vector, which consists of the above-mentioned construct, promoter, translational start signal, translational and transcriptional stop signal. Further, the invention concerns a recombinant host cell, containing the nucleic acid construct carried by the vector or integrated into the cell chromosome, and the E. coli BL21 strain containing said sequence of the nucleotides encoding the penicillin acylase carried in the pKXIP1, the pKLP3 or the pKLP6 plasmid.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_US8039604B2 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY SPIRITS SUGARS VINEGAR WINE |
| title | DNA sequence encoding penicillin acylase, novel recombinant recombinant DNA constructs and recombinant microorganisms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T05%3A30%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=STEPANEK%20VACLAV&rft.date=2011-10-18&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS8039604B2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |