Enzymatic process for the preparation of cephalosporanic 7 beta -(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli
Enzymatic process for the preparation of cephalosporanic 7beta-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | SALTO MALDONADO FRANCISO MELLADO DURAN ENCARNACION DIEZ GARCIA BRUNO GUISAN SEIJAS JOSE MANUEL ALONSO PALACIOS JORGE FUENTE BARREDO GARCIA LOPEZ JOSE LUIS CORTES RUBIO ESTRELLA |
| description | Enzymatic process for the preparation of cephalosporanic 7beta-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which codes for the enzyme D-aminoacid oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5' of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US6635458B2</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US6635458B2</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US6635458B23</originalsourceid><addsrcrecordid>eNqNjj1OA0EMhbehQMAdXEKxDfmBmpCIPlBHHo-XtbRrj8aTKJsDci5mIw5A9Wy979nvtvnZ6mUasQhBykbsDp1lKD3XnRPmapmCdUCcehzMk2XUir9A4ILQPi7bDeZg5ykcCyqOEvkJkCRCmGBkVJ_j88XRonTCEXh-yvDeVlrtytpZIjpf0SzfppZcHE6YBYMMdaz94pFqWhTYqecs1AsC2SD3zU2Hg_PDn941sNt-bj5aTnZgT0isXA5f-_V6sVquXt-eF_9AfgHu6WK3</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Enzymatic process for the preparation of cephalosporanic 7 beta -(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli</title><source>esp@cenet</source><creator>SALTO MALDONADO FRANCISO ; MELLADO DURAN ENCARNACION ; DIEZ GARCIA BRUNO ; GUISAN SEIJAS JOSE MANUEL ; ALONSO PALACIOS JORGE ; FUENTE BARREDO ; GARCIA LOPEZ JOSE LUIS ; CORTES RUBIO ESTRELLA</creator><creatorcontrib>SALTO MALDONADO FRANCISO ; MELLADO DURAN ENCARNACION ; DIEZ GARCIA BRUNO ; GUISAN SEIJAS JOSE MANUEL ; ALONSO PALACIOS JORGE ; FUENTE BARREDO ; GARCIA LOPEZ JOSE LUIS ; CORTES RUBIO ESTRELLA</creatorcontrib><description>Enzymatic process for the preparation of cephalosporanic 7beta-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which codes for the enzyme D-aminoacid oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5' of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography.</description><edition>7</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2003</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20031021&DB=EPODOC&CC=US&NR=6635458B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25551,76302</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20031021&DB=EPODOC&CC=US&NR=6635458B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SALTO MALDONADO FRANCISO</creatorcontrib><creatorcontrib>MELLADO DURAN ENCARNACION</creatorcontrib><creatorcontrib>DIEZ GARCIA BRUNO</creatorcontrib><creatorcontrib>GUISAN SEIJAS JOSE MANUEL</creatorcontrib><creatorcontrib>ALONSO PALACIOS JORGE</creatorcontrib><creatorcontrib>FUENTE BARREDO</creatorcontrib><creatorcontrib>GARCIA LOPEZ JOSE LUIS</creatorcontrib><creatorcontrib>CORTES RUBIO ESTRELLA</creatorcontrib><title>Enzymatic process for the preparation of cephalosporanic 7 beta -(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli</title><description>Enzymatic process for the preparation of cephalosporanic 7beta-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which codes for the enzyme D-aminoacid oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5' of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2003</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjj1OA0EMhbehQMAdXEKxDfmBmpCIPlBHHo-XtbRrj8aTKJsDci5mIw5A9Wy979nvtvnZ6mUasQhBykbsDp1lKD3XnRPmapmCdUCcehzMk2XUir9A4ILQPi7bDeZg5ykcCyqOEvkJkCRCmGBkVJ_j88XRonTCEXh-yvDeVlrtytpZIjpf0SzfppZcHE6YBYMMdaz94pFqWhTYqecs1AsC2SD3zU2Hg_PDn941sNt-bj5aTnZgT0isXA5f-_V6sVquXt-eF_9AfgHu6WK3</recordid><startdate>20031021</startdate><enddate>20031021</enddate><creator>SALTO MALDONADO FRANCISO</creator><creator>MELLADO DURAN ENCARNACION</creator><creator>DIEZ GARCIA BRUNO</creator><creator>GUISAN SEIJAS JOSE MANUEL</creator><creator>ALONSO PALACIOS JORGE</creator><creator>FUENTE BARREDO</creator><creator>GARCIA LOPEZ JOSE LUIS</creator><creator>CORTES RUBIO ESTRELLA</creator><scope>EVB</scope></search><sort><creationdate>20031021</creationdate><title>Enzymatic process for the preparation of cephalosporanic 7 beta -(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli</title><author>SALTO MALDONADO FRANCISO ; MELLADO DURAN ENCARNACION ; DIEZ GARCIA BRUNO ; GUISAN SEIJAS JOSE MANUEL ; ALONSO PALACIOS JORGE ; FUENTE BARREDO ; GARCIA LOPEZ JOSE LUIS ; CORTES RUBIO ESTRELLA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US6635458B23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2003</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SALTO MALDONADO FRANCISO</creatorcontrib><creatorcontrib>MELLADO DURAN ENCARNACION</creatorcontrib><creatorcontrib>DIEZ GARCIA BRUNO</creatorcontrib><creatorcontrib>GUISAN SEIJAS JOSE MANUEL</creatorcontrib><creatorcontrib>ALONSO PALACIOS JORGE</creatorcontrib><creatorcontrib>FUENTE BARREDO</creatorcontrib><creatorcontrib>GARCIA LOPEZ JOSE LUIS</creatorcontrib><creatorcontrib>CORTES RUBIO ESTRELLA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SALTO MALDONADO FRANCISO</au><au>MELLADO DURAN ENCARNACION</au><au>DIEZ GARCIA BRUNO</au><au>GUISAN SEIJAS JOSE MANUEL</au><au>ALONSO PALACIOS JORGE</au><au>FUENTE BARREDO</au><au>GARCIA LOPEZ JOSE LUIS</au><au>CORTES RUBIO ESTRELLA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Enzymatic process for the preparation of cephalosporanic 7 beta -(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli</title><date>2003-10-21</date><risdate>2003</risdate><abstract>Enzymatic process for the preparation of cephalosporanic 7beta-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli. The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to gene which codes for the enzyme D-aminoacid oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli; (IV) fusing at the extremity 5' of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_US6635458B2 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Enzymatic process for the preparation of cephalosporanic 7 beta -(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T04%3A04%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=SALTO%20MALDONADO%20FRANCISO&rft.date=2003-10-21&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS6635458B2%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |