Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein
PCT No. PCT/EP93/01763 Sec. 371 Date Feb. 27, 1995 Sec. 102(e) Date Feb. 27, 1995 PCT Filed Jul. 7, 1993 PCT Pub. No. WO94/01567 PCT Pub. Date Jan. 20, 1994A method is provided for immobilizing an enzyme, comprising immobilizing the enzyme or a functional part thereof to the cell wall of a microbial...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | SCHREUDER MAARTEN P VERRIPS CORNELIS T TOSCHKA HOLGER Y KLIS FRANCISCUS M |
| description | PCT No. PCT/EP93/01763 Sec. 371 Date Feb. 27, 1995 Sec. 102(e) Date Feb. 27, 1995 PCT Filed Jul. 7, 1993 PCT Pub. No. WO94/01567 PCT Pub. Date Jan. 20, 1994A method is provided for immobilizing an enzyme, comprising immobilizing the enzyme or a functional part thereof to the cell wall of a microbial cell using recombinant DNA techniques. The enzyme is immobilized by linking it to the C-terminal part of a protein that ensures anchoring in the cell wall. Also provided is a recombinant polynucleotide comprising a structural gene encoding an enzyme protein, a part of a gene encoding the C-terminal part of a protein capable of anchoring in a eukaryotic or prokaryotic cell wall, as well as a signal sequence, in addition to a chimeric protein encoded by the recombinant polynucleotide and a vector and a microorganism containing the polynucleotide. The microorganism is suitable for carrying out enzymatic processes on an industrial scale. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US6027910A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US6027910A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US6027910A3</originalsourceid><addsrcrecordid>eNqFi8EKwjAQRHvxIOo3uD8gVAXFo4jiUVDPZbtudCHJhiRF2q-3Re9eZmDem3FBl6jEKYHRCOKc1mKlE_8E9l3rOEFWyC8GYmvhjX2oAQQnFHsX7RfULYSoj4aGJ4Jpkqgfpszip8XIoE08-_WkmJ-Ot8N5wUErTgGJPefqft2Uq-1uWe7X_40PhkU9eQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein</title><source>esp@cenet</source><creator>SCHREUDER; MAARTEN P ; VERRIPS; CORNELIS T ; TOSCHKA; HOLGER Y ; KLIS; FRANCISCUS M</creator><creatorcontrib>SCHREUDER; MAARTEN P ; VERRIPS; CORNELIS T ; TOSCHKA; HOLGER Y ; KLIS; FRANCISCUS M</creatorcontrib><description>PCT No. PCT/EP93/01763 Sec. 371 Date Feb. 27, 1995 Sec. 102(e) Date Feb. 27, 1995 PCT Filed Jul. 7, 1993 PCT Pub. No. WO94/01567 PCT Pub. Date Jan. 20, 1994A method is provided for immobilizing an enzyme, comprising immobilizing the enzyme or a functional part thereof to the cell wall of a microbial cell using recombinant DNA techniques. The enzyme is immobilized by linking it to the C-terminal part of a protein that ensures anchoring in the cell wall. Also provided is a recombinant polynucleotide comprising a structural gene encoding an enzyme protein, a part of a gene encoding the C-terminal part of a protein capable of anchoring in a eukaryotic or prokaryotic cell wall, as well as a signal sequence, in addition to a chimeric protein encoded by the recombinant polynucleotide and a vector and a microorganism containing the polynucleotide. The microorganism is suitable for carrying out enzymatic processes on an industrial scale.</description><edition>7</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2000</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20000222&DB=EPODOC&CC=US&NR=6027910A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20000222&DB=EPODOC&CC=US&NR=6027910A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SCHREUDER; MAARTEN P</creatorcontrib><creatorcontrib>VERRIPS; CORNELIS T</creatorcontrib><creatorcontrib>TOSCHKA; HOLGER Y</creatorcontrib><creatorcontrib>KLIS; FRANCISCUS M</creatorcontrib><title>Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein</title><description>PCT No. PCT/EP93/01763 Sec. 371 Date Feb. 27, 1995 Sec. 102(e) Date Feb. 27, 1995 PCT Filed Jul. 7, 1993 PCT Pub. No. WO94/01567 PCT Pub. Date Jan. 20, 1994A method is provided for immobilizing an enzyme, comprising immobilizing the enzyme or a functional part thereof to the cell wall of a microbial cell using recombinant DNA techniques. The enzyme is immobilized by linking it to the C-terminal part of a protein that ensures anchoring in the cell wall. Also provided is a recombinant polynucleotide comprising a structural gene encoding an enzyme protein, a part of a gene encoding the C-terminal part of a protein capable of anchoring in a eukaryotic or prokaryotic cell wall, as well as a signal sequence, in addition to a chimeric protein encoded by the recombinant polynucleotide and a vector and a microorganism containing the polynucleotide. The microorganism is suitable for carrying out enzymatic processes on an industrial scale.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2000</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqFi8EKwjAQRHvxIOo3uD8gVAXFo4jiUVDPZbtudCHJhiRF2q-3Re9eZmDem3FBl6jEKYHRCOKc1mKlE_8E9l3rOEFWyC8GYmvhjX2oAQQnFHsX7RfULYSoj4aGJ4Jpkqgfpszip8XIoE08-_WkmJ-Ot8N5wUErTgGJPefqft2Uq-1uWe7X_40PhkU9eQ</recordid><startdate>20000222</startdate><enddate>20000222</enddate><creator>SCHREUDER; MAARTEN P</creator><creator>VERRIPS; CORNELIS T</creator><creator>TOSCHKA; HOLGER Y</creator><creator>KLIS; FRANCISCUS M</creator><scope>EVB</scope></search><sort><creationdate>20000222</creationdate><title>Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein</title><author>SCHREUDER; MAARTEN P ; VERRIPS; CORNELIS T ; TOSCHKA; HOLGER Y ; KLIS; FRANCISCUS M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US6027910A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2000</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SCHREUDER; MAARTEN P</creatorcontrib><creatorcontrib>VERRIPS; CORNELIS T</creatorcontrib><creatorcontrib>TOSCHKA; HOLGER Y</creatorcontrib><creatorcontrib>KLIS; FRANCISCUS M</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SCHREUDER; MAARTEN P</au><au>VERRIPS; CORNELIS T</au><au>TOSCHKA; HOLGER Y</au><au>KLIS; FRANCISCUS M</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein</title><date>2000-02-22</date><risdate>2000</risdate><abstract>PCT No. PCT/EP93/01763 Sec. 371 Date Feb. 27, 1995 Sec. 102(e) Date Feb. 27, 1995 PCT Filed Jul. 7, 1993 PCT Pub. No. WO94/01567 PCT Pub. Date Jan. 20, 1994A method is provided for immobilizing an enzyme, comprising immobilizing the enzyme or a functional part thereof to the cell wall of a microbial cell using recombinant DNA techniques. The enzyme is immobilized by linking it to the C-terminal part of a protein that ensures anchoring in the cell wall. Also provided is a recombinant polynucleotide comprising a structural gene encoding an enzyme protein, a part of a gene encoding the C-terminal part of a protein capable of anchoring in a eukaryotic or prokaryotic cell wall, as well as a signal sequence, in addition to a chimeric protein encoded by the recombinant polynucleotide and a vector and a microorganism containing the polynucleotide. The microorganism is suitable for carrying out enzymatic processes on an industrial scale.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_US6027910A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T09%3A33%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=SCHREUDER;%20MAARTEN%20P&rft.date=2000-02-22&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS6027910A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |