Aedes aegypti densovirus expression system
The present invention is directed to use of Aedes aegypti densonucleosis virus (Aedes DNV or AeDNV) as a heterologous gene expression system. The invention described herein provides promoters from the Aedes aegypti genome such as the p.05, p7 and p61 promoters to generate expression vectors with clo...
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| creator | AFANASIEV BORIS N THIBAULT KELLY J HIGGINS DAVID R BEATY BARRY J CARLSON JONATHAN ORED |
| description | The present invention is directed to use of Aedes aegypti densonucleosis virus (Aedes DNV or AeDNV) as a heterologous gene expression system. The invention described herein provides promoters from the Aedes aegypti genome such as the p.05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. The present invention further provides a kit containing the vectors of the invention for production of a recombinant protein. |
| format | Patent |
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The invention described herein provides promoters from the Aedes aegypti genome such as the p.05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. 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The invention described herein provides promoters from the Aedes aegypti genome such as the p.05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. The present invention further provides a kit containing the vectors of the invention for production of a recombinant protein.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Aedes aegypti densovirus expression system |
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