Method for regeneration of coniferous plants by somatic embryogenesis in culture media containing abscisic acid
The invention relates to a method for regeneration of plants of the genus Pinus by culturing explants of immature zygotic embryos on culture initiation medium containing abscisic acid, nutrients, growth hormones, sugar and a gelling agent to grow embryogenic tissue for cryopreservation. Culturing of...
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creator | HANDLEY, III LEVIS W |
description | The invention relates to a method for regeneration of plants of the genus Pinus by culturing explants of immature zygotic embryos on culture initiation medium containing abscisic acid, nutrients, growth hormones, sugar and a gelling agent to grow embryogenic tissue for cryopreservation. Culturing of the embryogenic tissue is continued on culture maintenance medium, embryo development medium, and germination medium. The germinated embryos are further converted to acclimatized plants for field planting. The method is well suited for producing clonal planting stock useful for reforestation. |
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Culturing of the embryogenic tissue is continued on culture maintenance medium, embryo development medium, and germination medium. The germinated embryos are further converted to acclimatized plants for field planting. The method is well suited for producing clonal planting stock useful for reforestation.</description><edition>6</edition><language>eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING</subject><creationdate>1997</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19971014&DB=EPODOC&CC=US&NR=5677185A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19971014&DB=EPODOC&CC=US&NR=5677185A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HANDLEY, III; LEVIS W</creatorcontrib><title>Method for regeneration of coniferous plants by somatic embryogenesis in culture media containing abscisic acid</title><description>The invention relates to a method for regeneration of plants of the genus Pinus by culturing explants of immature zygotic embryos on culture initiation medium containing abscisic acid, nutrients, growth hormones, sugar and a gelling agent to grow embryogenic tissue for cryopreservation. Culturing of the embryogenic tissue is continued on culture maintenance medium, embryo development medium, and germination medium. The germinated embryos are further converted to acclimatized plants for field planting. The method is well suited for producing clonal planting stock useful for reforestation.</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1997</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqFzDEOwjAMheEuDAg4A74AA0KlrAiBWJiAuXJTp1hq7ShOht6eVmJnesv_vWWhD0ofbcFrhEgdCUVMrALqwamwp6jZIPQoyaAZwXSYAgc0NHHUGRgbsIDLfcqRYKCWcbYJWVg6wMYc20TQcbsuFh57o81vV8X2dn1d7jsKWpMFdNNjqt_P8lhV-1N5Pvwvvl84Qvs</recordid><startdate>19971014</startdate><enddate>19971014</enddate><creator>HANDLEY, III; LEVIS W</creator><scope>EVB</scope></search><sort><creationdate>19971014</creationdate><title>Method for regeneration of coniferous plants by somatic embryogenesis in culture media containing abscisic acid</title><author>HANDLEY, III; LEVIS W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US5677185A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>1997</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><toplevel>online_resources</toplevel><creatorcontrib>HANDLEY, III; LEVIS W</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HANDLEY, III; LEVIS W</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for regeneration of coniferous plants by somatic embryogenesis in culture media containing abscisic acid</title><date>1997-10-14</date><risdate>1997</risdate><abstract>The invention relates to a method for regeneration of plants of the genus Pinus by culturing explants of immature zygotic embryos on culture initiation medium containing abscisic acid, nutrients, growth hormones, sugar and a gelling agent to grow embryogenic tissue for cryopreservation. Culturing of the embryogenic tissue is continued on culture maintenance medium, embryo development medium, and germination medium. The germinated embryos are further converted to acclimatized plants for field planting. The method is well suited for producing clonal planting stock useful for reforestation.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
title | Method for regeneration of coniferous plants by somatic embryogenesis in culture media containing abscisic acid |
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