Method for separation and recovery of biological materials

Disclosed is a centrifuge tube containing a porous selection means for selectively separating and recovering biological substances from gels, broths, or solutions containing the same, and thus concentrating them, the tube comprising separate upper and lower containers and means for joining the two c...

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Hauptverfasser: NOCHUMSON, SAMUEL, GOLDBERG, BRUCE S
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creator NOCHUMSON
SAMUEL
GOLDBERG
BRUCE S
description Disclosed is a centrifuge tube containing a porous selection means for selectively separating and recovering biological substances from gels, broths, or solutions containing the same, and thus concentrating them, the tube comprising separate upper and lower containers and means for joining the two containers to form a unitary device, the bottom of the upper container comprising a porous selection means, preferably a membrane containing a particulate substance which will selectively bind the biological substances while allowing unbound substances to pass through from the upper to lower container while being centrifuged. A method for recovering the biological substances in high concentration using the centrifuge tube is also provided, comprising introducing said gels, broths, or solutions containing the desired biological substances, centrifuging the same until the desired substance is bound to the selection means, and eluting the bound material from the selection means to recover the substance in concentrated form.
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subjects CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
TESTING
TRANSPORTING
title Method for separation and recovery of biological materials
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