ANIMAL CELL TRANSFORMANT, METHOD FOR OBTAINING THE TRANSFORMANT AND METHOD FOR PRODUCING DESIRED FOREIGN GENE PRODUCT BY USING THE TRANSFORMANT
By screening an animal cell transformant, which has been obtained by introducing into wild-type animal cells a wild-type DHFR gene and a structural gene which codes a desired useful substance, with a medium containing methotrexate (MTX) at a concentration of 50 mu M or lower, both genes can be ampli...
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| creator | TOMIOKA NOBORU OMAE FUMIO MINE ERIKO ISHII TOMOKO |
| description | By screening an animal cell transformant, which has been obtained by introducing into wild-type animal cells a wild-type DHFR gene and a structural gene which codes a desired useful substance, with a medium containing methotrexate (MTX) at a concentration of 50 mu M or lower, both genes can be amplified effectively with an MTX resistant strain and a stable amplified transformant can also be obtained. The screening may be repeated using an increasing concentration of MTX. It is also possible to obtain stably the desired foreign gene product in a high yield by using the thus-obtained amplified transformant. |
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The screening may be repeated using an increasing concentration of MTX. 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The screening may be repeated using an increasing concentration of MTX. It is also possible to obtain stably the desired foreign gene product in a high yield by using the thus-obtained amplified transformant.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1993</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZOh39PP0dfRRcHb18VEICXL0C3bzD_J19AvRUfB1DfHwd1EA8hX8nUIcPf08_dwVQjxcUZQpOPq5IKsMCPJ3CXUGqXRxDfYMcgWLunq6-ym4u_q5QqVDFJwiFUKDsZnHw8CalphTnMoLpbkZ5N1cQ5w9dFML8uNTiwsSk1PzUkviQ4NNjQwtzY0tHI0JqwAAEgw_tw</recordid><startdate>19930615</startdate><enddate>19930615</enddate><creator>TOMIOKA; NOBORU</creator><creator>OMAE; FUMIO</creator><creator>MINE; ERIKO</creator><creator>ISHII; TOMOKO</creator><scope>EVB</scope></search><sort><creationdate>19930615</creationdate><title>ANIMAL CELL TRANSFORMANT, METHOD FOR OBTAINING THE TRANSFORMANT AND METHOD FOR PRODUCING DESIRED FOREIGN GENE PRODUCT BY USING THE TRANSFORMANT</title><author>TOMIOKA; NOBORU ; OMAE; FUMIO ; MINE; ERIKO ; ISHII; TOMOKO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US5219738A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>1993</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>TOMIOKA; NOBORU</creatorcontrib><creatorcontrib>OMAE; FUMIO</creatorcontrib><creatorcontrib>MINE; ERIKO</creatorcontrib><creatorcontrib>ISHII; TOMOKO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TOMIOKA; NOBORU</au><au>OMAE; FUMIO</au><au>MINE; ERIKO</au><au>ISHII; TOMOKO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>ANIMAL CELL TRANSFORMANT, METHOD FOR OBTAINING THE TRANSFORMANT AND METHOD FOR PRODUCING DESIRED FOREIGN GENE PRODUCT BY USING THE TRANSFORMANT</title><date>1993-06-15</date><risdate>1993</risdate><abstract>By screening an animal cell transformant, which has been obtained by introducing into wild-type animal cells a wild-type DHFR gene and a structural gene which codes a desired useful substance, with a medium containing methotrexate (MTX) at a concentration of 50 mu M or lower, both genes can be amplified effectively with an MTX resistant strain and a stable amplified transformant can also be obtained. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | ANIMAL CELL TRANSFORMANT, METHOD FOR OBTAINING THE TRANSFORMANT AND METHOD FOR PRODUCING DESIRED FOREIGN GENE PRODUCT BY USING THE TRANSFORMANT |
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