Process for regenerating cereals
The present invention relates to the regeneration of cereals including barley, corn, wheat, rice and sorghum. The process comprises the steps of: (a) culturing tissue obtained from a cereal plant on an induction medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient...
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Zusammenfassung: | The present invention relates to the regeneration of cereals including barley, corn, wheat, rice and sorghum. The process comprises the steps of: (a) culturing tissue obtained from a cereal plant on an induction medium comprising mineral salts, vitamins, sucrose and a hormone in an amount sufficient to ensure callus formation, (b) culturing the callus on a series of media, said series comprising at least one medium and each medium comprises mineral salts, vitamins, sucrose and a hormone in an amount sufficient to insure differentiating to plantlets having shoots and roots at the completion of the series, and (c) culturing the plantlets on an establishment medium comprising mineral salts, vitamins and sucrose, whereby the plantlets are established so that they can be transplanted to soil. |
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