Immobilized ribonuclease and alkaline phosphatase
An insoluble, solid matrix carrying simultaneously several different enzymatic functions, is constituted by the conjoint association by irreversible binding on a previously activated matrix support, of a nuclease selected from the group of ribonucleases A, T1, T2, U2 and an alkaline phosphatease. Fr...
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| creator | GUISSANI BORN TRACHTENBERG ANNIE THANG MINH-NGUY |
| description | An insoluble, solid matrix carrying simultaneously several different enzymatic functions, is constituted by the conjoint association by irreversible binding on a previously activated matrix support, of a nuclease selected from the group of ribonucleases A, T1, T2, U2 and an alkaline phosphatease. Free activated groups of the matrix after binding of the enzymes, are neutralized by a free amino organic base. The support is selected from among non-denaturing supports effecting the irreversible physical adsorption of the enzymes, such as supports of glass or quartz beads, highly cross-linked gels of the agarose or cellulose type. Polymers AnUOH, AnCOH, AnGOH and/or oligonucleotides U, C, A or G, of predetermined lengths, can be obtained by fractionating the polyribonucleotides by incubation with the insoluble solid matrix. |
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Free activated groups of the matrix after binding of the enzymes, are neutralized by a free amino organic base. The support is selected from among non-denaturing supports effecting the irreversible physical adsorption of the enzymes, such as supports of glass or quartz beads, highly cross-linked gels of the agarose or cellulose type. 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Polymers AnUOH, AnCOH, AnGOH and/or oligonucleotides U, C, A or G, of predetermined lengths, can be obtained by fractionating the polyribonucleotides by incubation with the insoluble solid matrix.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DERIVATIVES THEREOF</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC</subject><subject>GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOSIDES</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC CHEMISTRY</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>SUGARS</subject><subject>TECHNICAL SUBJECTS COVERED BY FORMER USPC</subject><subject>TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS</subject><subject>TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1977</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDD0zM3NT8rMyaxKTVEoykzKzytNzklNLE5VSMxLUUjMyU7MycxLVSjIyC8uyEgsAUrwMLCmJeYUp_JCaW4GeTfXEGcP3dSC_PjU4oLE5NS81JL40GATA2NLYwsjR2PCKgAjRCuB</recordid><startdate>19770802</startdate><enddate>19770802</enddate><creator>GUISSANI BORN TRACHTENBERG; ANNIE</creator><creator>THANG; MINH-NGUY</creator><scope>EVB</scope></search><sort><creationdate>19770802</creationdate><title>Immobilized ribonuclease and alkaline phosphatase</title><author>GUISSANI BORN TRACHTENBERG; ANNIE ; THANG; MINH-NGUY</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US4039382A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>1977</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DERIVATIVES THEREOF</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC</topic><topic>GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOSIDES</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC CHEMISTRY</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>SUGARS</topic><topic>TECHNICAL SUBJECTS COVERED BY FORMER USPC</topic><topic>TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS</topic><topic>TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>GUISSANI BORN TRACHTENBERG; ANNIE</creatorcontrib><creatorcontrib>THANG; MINH-NGUY</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>GUISSANI BORN TRACHTENBERG; ANNIE</au><au>THANG; MINH-NGUY</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Immobilized ribonuclease and alkaline phosphatase</title><date>1977-08-02</date><risdate>1977</risdate><abstract>An insoluble, solid matrix carrying simultaneously several different enzymatic functions, is constituted by the conjoint association by irreversible binding on a previously activated matrix support, of a nuclease selected from the group of ribonucleases A, T1, T2, U2 and an alkaline phosphatease. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS VINEGAR WINE |
| title | Immobilized ribonuclease and alkaline phosphatase |
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