METHOD FOR QUANTIFYING ENDOTOXINS IN A BIOLOGICAL SAMPLE

METHOD FOR QUANTIFYING ENDOTOXINS IN A BIOLOGICAL SAMPLE

A method for quantifying endotoxins in a biological sample with an analysis medium added to the field of view of an imager, the analysis medium including at least one analysis chamber and a plurality of reference chambers, including the steps of, for each of a plurality of measuring times of a measu...

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Hauptverfasser: GOUJARD, Matthieu, PINSTON, Frédéric, BROYER, Patrick
Format: Patent
Sprache:eng
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator GOUJARD, Matthieu
PINSTON, Frédéric
BROYER, Patrick
description A method for quantifying endotoxins in a biological sample with an analysis medium added to the field of view of an imager, the analysis medium including at least one analysis chamber and a plurality of reference chambers, including the steps of, for each of a plurality of measuring times of a measuring period: a) acquiring an image and determining light intensity valves in the reference and analysis chambers; b) determining a calibration relationship connecting the light intensity value and the concentration at this measuring time; c) determination of at least one endotoxin concentration in a biological sample from said calibration relationship and the intensity value in the analysis chamber at said measurement time; the method subsequently including the step of d) determining a temporal evolution of an endotoxin concentration in the biological sample over the course of the measuring period from the endotoxin concentration for a plurality of measurement times.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title METHOD FOR QUANTIFYING ENDOTOXINS IN A BIOLOGICAL SAMPLE
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