TAQ ENZYME MUTANT, PREPARATION METHOD, AND APPLICATION THEREOF
The present application discloses mutants of Taq enzyme, a method of preparation thereof and an application thereof. In the present application, the mutants of Taq enzyme comprises: an amino acid sequence having at least 70% identity to the amino acid sequence shown in SEQ ID NO:2; amino acid sequen...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | LIAN, Xianlan ZHANG, Wei LU, Xuelan JIANG, Xiwen |
| description | The present application discloses mutants of Taq enzyme, a method of preparation thereof and an application thereof. In the present application, the mutants of Taq enzyme comprises: an amino acid sequence having at least 70% identity to the amino acid sequence shown in SEQ ID NO:2; amino acid sequence is mutated at one or more sites selected from the group consisting of: D335, G499, E634, F769 and a combination thereof. The mutants of Taq enzyme provided in the present application have high amplification activity, yield more amplification products compared to wild-type Taq enzyme at the same number of PCR cycles, are both whole blood and high salt resistant, and do not lose 5′˜3′ exonuclease activity. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US2024247241A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US2024247241A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US2024247241A13</originalsourceid><addsrcrecordid>eNrjZLALcQxUcPWLivR1VfANDXH0C9FRCAhyDXAMcgzx9PdT8HUN8fB30VFw9HNRcAwI8PF0hoiHeLgGufq78TCwpiXmFKfyQmluBmU31xBnD93Ugvz41OKCxOTUvNSS-NBgIwMjEyMTcyMTQ0dDY-JUAQD9ISr6</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>TAQ ENZYME MUTANT, PREPARATION METHOD, AND APPLICATION THEREOF</title><source>esp@cenet</source><creator>LIAN, Xianlan ; ZHANG, Wei ; LU, Xuelan ; JIANG, Xiwen</creator><creatorcontrib>LIAN, Xianlan ; ZHANG, Wei ; LU, Xuelan ; JIANG, Xiwen</creatorcontrib><description>The present application discloses mutants of Taq enzyme, a method of preparation thereof and an application thereof. In the present application, the mutants of Taq enzyme comprises: an amino acid sequence having at least 70% identity to the amino acid sequence shown in SEQ ID NO:2; amino acid sequence is mutated at one or more sites selected from the group consisting of: D335, G499, E634, F769 and a combination thereof. The mutants of Taq enzyme provided in the present application have high amplification activity, yield more amplification products compared to wild-type Taq enzyme at the same number of PCR cycles, are both whole blood and high salt resistant, and do not lose 5′˜3′ exonuclease activity.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240725&DB=EPODOC&CC=US&NR=2024247241A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240725&DB=EPODOC&CC=US&NR=2024247241A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIAN, Xianlan</creatorcontrib><creatorcontrib>ZHANG, Wei</creatorcontrib><creatorcontrib>LU, Xuelan</creatorcontrib><creatorcontrib>JIANG, Xiwen</creatorcontrib><title>TAQ ENZYME MUTANT, PREPARATION METHOD, AND APPLICATION THEREOF</title><description>The present application discloses mutants of Taq enzyme, a method of preparation thereof and an application thereof. In the present application, the mutants of Taq enzyme comprises: an amino acid sequence having at least 70% identity to the amino acid sequence shown in SEQ ID NO:2; amino acid sequence is mutated at one or more sites selected from the group consisting of: D335, G499, E634, F769 and a combination thereof. The mutants of Taq enzyme provided in the present application have high amplification activity, yield more amplification products compared to wild-type Taq enzyme at the same number of PCR cycles, are both whole blood and high salt resistant, and do not lose 5′˜3′ exonuclease activity.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLALcQxUcPWLivR1VfANDXH0C9FRCAhyDXAMcgzx9PdT8HUN8fB30VFw9HNRcAwI8PF0hoiHeLgGufq78TCwpiXmFKfyQmluBmU31xBnD93Ugvz41OKCxOTUvNSS-NBgIwMjEyMTcyMTQ0dDY-JUAQD9ISr6</recordid><startdate>20240725</startdate><enddate>20240725</enddate><creator>LIAN, Xianlan</creator><creator>ZHANG, Wei</creator><creator>LU, Xuelan</creator><creator>JIANG, Xiwen</creator><scope>EVB</scope></search><sort><creationdate>20240725</creationdate><title>TAQ ENZYME MUTANT, PREPARATION METHOD, AND APPLICATION THEREOF</title><author>LIAN, Xianlan ; ZHANG, Wei ; LU, Xuelan ; JIANG, Xiwen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2024247241A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIAN, Xianlan</creatorcontrib><creatorcontrib>ZHANG, Wei</creatorcontrib><creatorcontrib>LU, Xuelan</creatorcontrib><creatorcontrib>JIANG, Xiwen</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIAN, Xianlan</au><au>ZHANG, Wei</au><au>LU, Xuelan</au><au>JIANG, Xiwen</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>TAQ ENZYME MUTANT, PREPARATION METHOD, AND APPLICATION THEREOF</title><date>2024-07-25</date><risdate>2024</risdate><abstract>The present application discloses mutants of Taq enzyme, a method of preparation thereof and an application thereof. In the present application, the mutants of Taq enzyme comprises: an amino acid sequence having at least 70% identity to the amino acid sequence shown in SEQ ID NO:2; amino acid sequence is mutated at one or more sites selected from the group consisting of: D335, G499, E634, F769 and a combination thereof. The mutants of Taq enzyme provided in the present application have high amplification activity, yield more amplification products compared to wild-type Taq enzyme at the same number of PCR cycles, are both whole blood and high salt resistant, and do not lose 5′˜3′ exonuclease activity.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_US2024247241A1 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | TAQ ENZYME MUTANT, PREPARATION METHOD, AND APPLICATION THEREOF |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T16%3A09%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LIAN,%20Xianlan&rft.date=2024-07-25&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS2024247241A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |