BACILLUS VELEZENSIS STRAIN BRED THROUGH ULTRASOUND-ASSISTED ADAPTIVE EVOLUTION, AND USE THEREOF

A Bacillus velezensis (B. velezensis) strain bred through ultrasound-assisted adaptive evolution and use thereof are provided. In the present disclosure, a B. velezensis strain (B. velezensis CICC 23571) is used as a starting strain, and an ultrasound-assisted adaptive evolution process is performed...

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Hauptverfasser: LI, Yunliang, WANG, Xiaojing, XIE, Pengfei, JI, Yaoyong, ZHOU, Anqi, LIU, Xiaoshuang, MA, Haile, RUAN, Siyu
Format: Patent
Sprache:eng
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Zusammenfassung:A Bacillus velezensis (B. velezensis) strain bred through ultrasound-assisted adaptive evolution and use thereof are provided. In the present disclosure, a B. velezensis strain (B. velezensis CICC 23571) is used as a starting strain, and an ultrasound-assisted adaptive evolution process is performed, where a proportion of organic nitrogen in a medium is continuously reduced and proportions of inorganic nitrogen and a sugar source are increased to obtain a B. velezensis mutant strain with a high polypeptide yield. Different metabolic pathways in the mutant strain are enhanced, such that the mutant strain can efficiently convert inorganic nitrogen into organic nitrogen or reduce the deaminization of organic nitrogen, and finally a yield of polypeptides is greatly increased.