METHOD FOR DETERMINING THE PHASE AND/OR REFRACTIVE INDEX OF A REGION OF AN OBJECT, AND MICROSCOPE FOR DETERMINING THE PHASE AND/OR REFRACTIVE INDEX OF A REGION OF AN OBJECT

In a method for determining the phase and/or refractive index of a region of an object, the object region is illuminated with coherent or partly coherent light and is imaged into the image plane a number of times with different imaging properties and is recorded in order to obtain a plurality of intensity recordings of the object region. The phase and/or refractive index determination is carried out based upon the plurality of intensity recordings. The different imaging properties differ at least in terms of different phase shifts which are additionally introduced into the imaging beam path, and which are generated differently than by changing the focusing when carrying out the recordings. The different phase shifts which are additionally introduced into the imaging beam path are effected by introducing at least one optical element into the objective and/or manipulating at least one optical element of the objective.