METHOD OF PRODUCING HAIRPIN SINGLE-STRANDED RNA MOLECULES

METHOD OF PRODUCING HAIRPIN SINGLE-STRANDED RNA MOLECULES

A method produces a hairpin single-stranded RNA molecule capable of inhibiting expression of a target gene, including the step of reacting a first single-stranded oligo-RNA molecule represented by formula (I) with a second single-stranded oligo-RNA molecule represented by formula (II) in a mixed sol...

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Hauptverfasser: Fujita, Tatsuya, Aoki, Eriko, Inada, Hideaki, Ohgi, Tadaaki, Koshimoto, Kyohei, Iseki, Katsuhiko, Okimura, Keiichi, Kunishima, Munetaka
Format: Patent
Sprache:eng
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Zusammenfassung:A method produces a hairpin single-stranded RNA molecule capable of inhibiting expression of a target gene, including the step of reacting a first single-stranded oligo-RNA molecule represented by formula (I) with a second single-stranded oligo-RNA molecule represented by formula (II) in a mixed solvent including a buffer solution and a hydrophilic organic solvent in the presence of a dehydration condensation agent: 5′-Xc-Lx1 . . . (I) and Lx2-X-Y-Ly-Yc-3′ . . . (II), wherein the dehydration condensation agent is selected from the group consisting of a triazine-based dehydration condensation agent, a uronium-based dehydration condensation agent including an N-hydroxy nitrogen-containing aromatic ring structure, a carbodiimide-based dehydration condensation agent, a 2-halopyridinium-based dehydration condensation agent, and a formamidinium-based dehydration condensation agent.