DEOXYRIBONUCLEIC ACID-BASED BIOSENSOR AND ASSOCIATED METHODS

The present disclosure relates to biosensors comprising a sensor region, a linker region, and a reporter region. The sensor region is a DNA aptamer and includes a target domain configured to bind to a target and a reporter domain configured to bind to a reporter. The linker domain operably connects...

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Bibliographische Detailangaben
Hauptverfasser:	Reeck, Jonathon C, Shults, Nicholas H, Covert, Hunter J, Li, Shiwei, Burden, Steven J, Pollock, Tanner B, Poe, Aidan M, Gibard Bohachek, Clémentine FN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure relates to biosensors comprising a sensor region, a linker region, and a reporter region. The sensor region is a DNA aptamer and includes a target domain configured to bind to a target and a reporter domain configured to bind to a reporter. The linker domain operably connects the target domain to the reporter domain. Binding of the target to the target domain results in a conformational change, such as an allosteric change, to the aptamer resulting in second signal emitted by the reporter that differs from a first signal emitted by the reporter compared to the target unbound state. Methods of selecting biosensors and their use to detect the presence of a target in a sample are provided herein.