DETECTION METHOD FOR A TARGET NUCLEIC ACID AND KIT

A detection method for a target nucleic acid comprising: a first step of contacting a target nucleic acid with a first guide RNA and a first Cas protein; a second step of contacting the target nucleic acid with a second guide RNA and a second Cas protein; and a third step of detecting a complex comp...

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Bibliographische Detailangaben
Hauptverfasser:	Yano, Tetsuya, Minami, Masato, Okano, Mie, Yamamoto, Yoji
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A detection method for a target nucleic acid comprising: a first step of contacting a target nucleic acid with a first guide RNA and a first Cas protein; a second step of contacting the target nucleic acid with a second guide RNA and a second Cas protein; and a third step of detecting a complex comprising the target nucleic acid, the first guide RNA, the first Cas protein, the second guid RNA, and the second Cas protein, wherein, in the complex, the first guide RNA and the first Cas protein are bound to the first base sequence, and the second guide RNA and the second Cas protein are bound to the second base sequence.