CELL CULTURE SUBSTRATE, METHOD FOR PRODUCING CELL CULTURE SUBSTRATE, AND METHOD FOR PRODUCING SPHEROIDS
There are provided a cell culture substrate that enables efficient spheroid formation for cells and can form spheroids having a uniform size and an arbitrary shape at a high cell viability, a method for producing the cell culture substrate, and a method of producing spheroids in which the cell cultu...
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| creator | KUNO, Goshi IMAIZUMI, Yu |
| description | There are provided a cell culture substrate that enables efficient spheroid formation for cells and can form spheroids having a uniform size and an arbitrary shape at a high cell viability, a method for producing the cell culture substrate, and a method of producing spheroids in which the cell culture substrate is used and a cell viability inside of the spheroids is excellent. A cell culture substrate includes a substrate and a stimulus-responsive polymer coated on the substrate, wherein the stimulus-responsive polymer is a block copolymer having a water-insoluble block segment and a stimulus-responsive block segment, and the cell culture substrate includes the following two regions (A) and (B): (A) an island-shaped region having cell proliferation properties and stimulus responsiveness and having an area of 0.001 to 5 mm2; and (B) a region adjacent to the region (A) and having no cell proliferation properties. |
| format | Patent |
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A cell culture substrate includes a substrate and a stimulus-responsive polymer coated on the substrate, wherein the stimulus-responsive polymer is a block copolymer having a water-insoluble block segment and a stimulus-responsive block segment, and the cell culture substrate includes the following two regions (A) and (B): (A) an island-shaped region having cell proliferation properties and stimulus responsiveness and having an area of 0.001 to 5 mm2; and (B) a region adjacent to the region (A) and having no cell proliferation properties.</description><language>eng</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211118&DB=EPODOC&CC=US&NR=2021355424A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211118&DB=EPODOC&CC=US&NR=2021355424A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KUNO, Goshi</creatorcontrib><creatorcontrib>IMAIZUMI, Yu</creatorcontrib><title>CELL CULTURE SUBSTRATE, METHOD FOR PRODUCING CELL CULTURE SUBSTRATE, AND METHOD FOR PRODUCING SPHEROIDS</title><description>There are provided a cell culture substrate that enables efficient spheroid formation for cells and can form spheroids having a uniform size and an arbitrary shape at a high cell viability, a method for producing the cell culture substrate, and a method of producing spheroids in which the cell culture substrate is used and a cell viability inside of the spheroids is excellent. A cell culture substrate includes a substrate and a stimulus-responsive polymer coated on the substrate, wherein the stimulus-responsive polymer is a block copolymer having a water-insoluble block segment and a stimulus-responsive block segment, and the cell culture substrate includes the following two regions (A) and (B): (A) an island-shaped region having cell proliferation properties and stimulus responsiveness and having an area of 0.001 to 5 mm2; and (B) a region adjacent to the region (A) and having no cell proliferation properties.</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZEh3dvXxUXAO9QkJDXJVCA51Cg4Jcgxx1VHwdQ3x8HdRcPMPUggI8ncJdfb0c1fApdjRzwW7huAAD9cgf0-XYB4G1rTEnOJUXijNzaDs5hri7KGbWpAfn1pckJicmpdaEh8abGRgZGhsampiZOJoaEycKgDQjTZe</recordid><startdate>20211118</startdate><enddate>20211118</enddate><creator>KUNO, Goshi</creator><creator>IMAIZUMI, Yu</creator><scope>EVB</scope></search><sort><creationdate>20211118</creationdate><title>CELL CULTURE SUBSTRATE, METHOD FOR PRODUCING CELL CULTURE SUBSTRATE, AND METHOD FOR PRODUCING SPHEROIDS</title><author>KUNO, Goshi ; IMAIZUMI, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2021355424A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2021</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KUNO, Goshi</creatorcontrib><creatorcontrib>IMAIZUMI, Yu</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KUNO, Goshi</au><au>IMAIZUMI, Yu</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>CELL CULTURE SUBSTRATE, METHOD FOR PRODUCING CELL CULTURE SUBSTRATE, AND METHOD FOR PRODUCING SPHEROIDS</title><date>2021-11-18</date><risdate>2021</risdate><abstract>There are provided a cell culture substrate that enables efficient spheroid formation for cells and can form spheroids having a uniform size and an arbitrary shape at a high cell viability, a method for producing the cell culture substrate, and a method of producing spheroids in which the cell culture substrate is used and a cell viability inside of the spheroids is excellent. A cell culture substrate includes a substrate and a stimulus-responsive polymer coated on the substrate, wherein the stimulus-responsive polymer is a block copolymer having a water-insoluble block segment and a stimulus-responsive block segment, and the cell culture substrate includes the following two regions (A) and (B): (A) an island-shaped region having cell proliferation properties and stimulus responsiveness and having an area of 0.001 to 5 mm2; and (B) a region adjacent to the region (A) and having no cell proliferation properties.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | CELL CULTURE SUBSTRATE, METHOD FOR PRODUCING CELL CULTURE SUBSTRATE, AND METHOD FOR PRODUCING SPHEROIDS |
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