Method of Fluorescent Detection of Isothermal Loop-Mediated Amplification (LAMP) of a Target Nucleic Acid, Oligonucleotides and Kits Thereof

A method of fluorescent detection of isothermal loop-mediated amplification (LAMP) of a target nucleic acid, oligonucleotides and kits thereofThe invention concerns a method for detecting isothermal loop-mediated (LAMP) amplification of a target nucleic acid sequence which is based on the fluorescen...

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Bibliographische Detailangaben
Hauptverfasser: Minnucci, Giulia, Mesturini, Riccardo
Format: Patent
Sprache:eng
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Zusammenfassung:A method of fluorescent detection of isothermal loop-mediated amplification (LAMP) of a target nucleic acid, oligonucleotides and kits thereofThe invention concerns a method for detecting isothermal loop-mediated (LAMP) amplification of a target nucleic acid sequence which is based on the fluorescence resonance energy transfer (FRET) mechanism. The invention also concerns a set of oligonucleotides and a kit adapted for carrying out the LAMP-FRET method of the invention.