PUPAE SINGULATOR AND SEX SORTER

Devices, systems, and methods for isolating an insect pupa from an aqueous solution including a plurality of insect pupae. An isolation device may include a primary channel through which the aqueous solution and insect pupae travel, an outlet, and a secondary channel that intersects the primary channel. The secondary channel may transport a fluid to add it to the primary channel between an inlet and the outlet. When the sealable outlet is closed, an insect pupa located downstream of the intersection of the primary and secondary channels remains stationary while any insect pupae upstream of the intersection are pushed towards the inlet. The systems and methods described herein may also provide for identifying and sorting insect pupae.