PURIFICATION OF POLYMERASE COMPLEXES

Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or n...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: CRISALLI, Peter, DAVIS, Randall W, CECH, Cynthia, MABRY, Joshua N, CRAIG, Timothy Kellogg, CHECHELSKI JOHNSTON, Natalie B, DHIMAN, Kirti, BIBILLO, Arkadiusz, FRANKLIN, Helen, AYER, Aruna
Format: Patent
Sprache:eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator CRISALLI, Peter
DAVIS, Randall W
CECH, Cynthia
MABRY, Joshua N
CRAIG, Timothy Kellogg
CHECHELSKI JOHNSTON, Natalie B
DHIMAN, Kirti
BIBILLO, Arkadiusz
FRANKLIN, Helen
AYER, Aruna
description Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_US2020277581A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US2020277581A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_US2020277581A13</originalsourceid><addsrcrecordid>eNrjZFAJCA3ydPN0dgzx9PdT8HdTCPD3ifR1DXIMdlVw9vcN8HGNcA3mYWBNS8wpTuWF0twMym6uIc4euqkF-fGpxQWJyal5qSXxocFGBkBobm5qYehoaEycKgAmwSQ2</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>PURIFICATION OF POLYMERASE COMPLEXES</title><source>esp@cenet</source><creator>CRISALLI, Peter ; DAVIS, Randall W ; CECH, Cynthia ; MABRY, Joshua N ; CRAIG, Timothy Kellogg ; CHECHELSKI JOHNSTON, Natalie B ; DHIMAN, Kirti ; BIBILLO, Arkadiusz ; FRANKLIN, Helen ; AYER, Aruna</creator><creatorcontrib>CRISALLI, Peter ; DAVIS, Randall W ; CECH, Cynthia ; MABRY, Joshua N ; CRAIG, Timothy Kellogg ; CHECHELSKI JOHNSTON, Natalie B ; DHIMAN, Kirti ; BIBILLO, Arkadiusz ; FRANKLIN, Helen ; AYER, Aruna</creatorcontrib><description>Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20200903&amp;DB=EPODOC&amp;CC=US&amp;NR=2020277581A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20200903&amp;DB=EPODOC&amp;CC=US&amp;NR=2020277581A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CRISALLI, Peter</creatorcontrib><creatorcontrib>DAVIS, Randall W</creatorcontrib><creatorcontrib>CECH, Cynthia</creatorcontrib><creatorcontrib>MABRY, Joshua N</creatorcontrib><creatorcontrib>CRAIG, Timothy Kellogg</creatorcontrib><creatorcontrib>CHECHELSKI JOHNSTON, Natalie B</creatorcontrib><creatorcontrib>DHIMAN, Kirti</creatorcontrib><creatorcontrib>BIBILLO, Arkadiusz</creatorcontrib><creatorcontrib>FRANKLIN, Helen</creatorcontrib><creatorcontrib>AYER, Aruna</creatorcontrib><title>PURIFICATION OF POLYMERASE COMPLEXES</title><description>Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZFAJCA3ydPN0dgzx9PdT8HdTCPD3ifR1DXIMdlVw9vcN8HGNcA3mYWBNS8wpTuWF0twMym6uIc4euqkF-fGpxQWJyal5qSXxocFGBkBobm5qYehoaEycKgAmwSQ2</recordid><startdate>20200903</startdate><enddate>20200903</enddate><creator>CRISALLI, Peter</creator><creator>DAVIS, Randall W</creator><creator>CECH, Cynthia</creator><creator>MABRY, Joshua N</creator><creator>CRAIG, Timothy Kellogg</creator><creator>CHECHELSKI JOHNSTON, Natalie B</creator><creator>DHIMAN, Kirti</creator><creator>BIBILLO, Arkadiusz</creator><creator>FRANKLIN, Helen</creator><creator>AYER, Aruna</creator><scope>EVB</scope></search><sort><creationdate>20200903</creationdate><title>PURIFICATION OF POLYMERASE COMPLEXES</title><author>CRISALLI, Peter ; DAVIS, Randall W ; CECH, Cynthia ; MABRY, Joshua N ; CRAIG, Timothy Kellogg ; CHECHELSKI JOHNSTON, Natalie B ; DHIMAN, Kirti ; BIBILLO, Arkadiusz ; FRANKLIN, Helen ; AYER, Aruna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US2020277581A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CRISALLI, Peter</creatorcontrib><creatorcontrib>DAVIS, Randall W</creatorcontrib><creatorcontrib>CECH, Cynthia</creatorcontrib><creatorcontrib>MABRY, Joshua N</creatorcontrib><creatorcontrib>CRAIG, Timothy Kellogg</creatorcontrib><creatorcontrib>CHECHELSKI JOHNSTON, Natalie B</creatorcontrib><creatorcontrib>DHIMAN, Kirti</creatorcontrib><creatorcontrib>BIBILLO, Arkadiusz</creatorcontrib><creatorcontrib>FRANKLIN, Helen</creatorcontrib><creatorcontrib>AYER, Aruna</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CRISALLI, Peter</au><au>DAVIS, Randall W</au><au>CECH, Cynthia</au><au>MABRY, Joshua N</au><au>CRAIG, Timothy Kellogg</au><au>CHECHELSKI JOHNSTON, Natalie B</au><au>DHIMAN, Kirti</au><au>BIBILLO, Arkadiusz</au><au>FRANKLIN, Helen</au><au>AYER, Aruna</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PURIFICATION OF POLYMERASE COMPLEXES</title><date>2020-09-03</date><risdate>2020</risdate><abstract>Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language eng
recordid cdi_epo_espacenet_US2020277581A1
source esp@cenet
subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title PURIFICATION OF POLYMERASE COMPLEXES
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T18%3A25%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=CRISALLI,%20Peter&rft.date=2020-09-03&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EUS2020277581A1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true