MULTIPLE CYCLOADDITION REACTIONS FOR LABELING OF MOLECULES

MULTIPLE CYCLOADDITION REACTIONS FOR LABELING OF MOLECULES

The present invention relates to methods for linking tetrazines with dienophiles to establish at least two linkages by sequentially performing at least two cycloaddition reactions. The methods in particular allow establishing multi-labeling strategies. In particular, the invention relates to methods...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Valle Aramburu, Iker, Schultz, Carsten, Plass, Tilman, Lemke, Edward, Hoffmann, Jan-Erik, Nikic, Ivana
Format: Patent
Sprache:eng
Schlagworte:
ACYCLIC OR CARBOCYCLIC COMPOUNDS
CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:The present invention relates to methods for linking tetrazines with dienophiles to establish at least two linkages by sequentially performing at least two cycloaddition reactions. The methods in particular allow establishing multi-labeling strategies. In particular, the invention relates to methods for forming linkages by cycloaddition reactions, wherein the method comprises reacting a first alkyl-substituted tetrazine with a first dienophile comprising a irans-cyclooctenyl group followed by reacting a second tetrazine with a second dienophile comprising a cyclooctynyl group, wherein the reaction of the first tetrazine with the first dienophile proceeds in the presence of the second dienophile.