DIRECT DETECTION OF SINGLE MOLECULES ON MICROPARTICLES

The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provi...

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Bibliographische Detailangaben
Hauptverfasser:	Pope, Mark R, Prostko, John, Qiu, Xiaoxing, Calfin, Brenda, Tetin, Sergey Y, Swift, Kerry M, Bogdan, Felicia, Macdonald, Patrick J, Lin, Zhen, Haack, Richard, Ruan, Qiaoqiao
Format:	Patent
Sprache:	eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.