SYSTEM AND METHOD FOR TIME-RESOLVED FLUOROIMMUNOASSAY DETECTION

A system (100) for time-resolved fluoroimmunoassay detection is disclosed. The system (100) comprises a receptacle (110) with a sample volume adapted for receiving a sample (99) therein; a light source (120) adapted to emit pulsed excitation light (200); illumination optics (130) adapted to collect the pulsed excitation light (200) from the light source (120) and to deliver said pulsed excitation light (200) to the sample volume in the receptacle (110); a detection device (140) adapted for gated detection of fluorescence radiation (210) at least in a detection spectral range; detection optics (150) adapted to collect fluorescence radiation (210) from the receptacle (110) at least in the detection spectral range and deliver said fluorescence radiation (210) to the detection device (140); and an optical filter device (160) configured for the separation of excitation light (200) and detection light (210). The light source (120) is a light emitting diode with a peak intensity emission at a wavelength below 355 nm. The system (100) further comprises a control unit (101) configured to at least control timing of a detection cycle. The detection cycle comprises an excitation pulse emitted by the light emitting diode (120), followed by a detection period for the detection of fluorescence radiation (210) by the detection device (140), wherein the detection period is separated from the excitation pulse by a detection delay. A pulse duration of the excitation pulse is at least 10 μs. According to a further aspect, a method for time-resolved fluoroimmunoassay detection is provided.