LIVE-CELL COMPUTED TOMOGRAPHY

LIVE-CELL COMPUTED TOMOGRAPHY

Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-involves the acquisition of a series of two dimensional (2...

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Hauptverfasser: Chao, Shih-Hui Joseph, Myers, Jakrey, Johnson, Roger, Kelbauskas, Laimonas, Wang, Hong, Elango, Iniyan Soundappa, Meldrum, Deirdre, Houkal, Jeff, Ashcroft, Brian, Smith, Dean, Shetty, Rishabh
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INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator Chao, Shih-Hui Joseph
Myers, Jakrey
Johnson, Roger
Kelbauskas, Laimonas
Wang, Hong
Elango, Iniyan Soundappa
Meldrum, Deirdre
Houkal, Jeff
Ashcroft, Brian
Smith, Dean
Shetty, Rishabh
description Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-involves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.
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subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title LIVE-CELL COMPUTED TOMOGRAPHY
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